Acid production in dental plaque after exposure to probiotic bacteria

Mette K Keller, Svante Twetman, Mette K Keller, Svante Twetman

Abstract

Background: The increasing interest in probiotic lactobacilli in health maintenance has raised the question of potential risks. One possible side effect could be an increased acidogenicity in dental plaque. The aim of this study was to investigate the effect of probiotic lactobacilli on plaque lactic acid (LA) production in vitro and in vivo.

Methods: In the first part (A), suspensions of two lactobacilli strains (L. reuteri DSM 17938, L. plantarum 299v) were added to suspensions of supragingival dental plaque collected from healthy young adults (n=25). LA production after fermentation with either xylitol or fructose was analyzed. In the second part (B), subjects (n=18) were given lozenges with probiotic lactobacilli (L. reuteri DSM 17938 and ATCC PTA 5289) or placebo for two weeks in a double-blinded, randomized cross-over trial. The concentration of LA in supragingival plaque samples was determined at baseline and after 2 weeks. Salivary counts of mutans streptococci (MS) and lactobacilli were estimated with chair-side methods.

Results: Plaque suspensions with L. reuteri DSM 17938 produced significantly less LA compared with L. plantarum 299v or controls (p<0.05). Fructose gave higher LA concentrations than xylitol. In part B, there were no significant differences in LA production between baseline and follow up in any of the groups and no differences between test and placebo were displayed. The salivary MS counts were not significantly altered during the intervention but the lactobacilli counts increased significantly in the test group (p<0.05).

Conclusion: Lactic acid production in suspensions of plaque and probiotic lactobacilli was strain-dependant and the present study provides no evidence of an increase in plaque acidity by the supply of selected probiotic lactobacilli when challenged by fructose or xylitol. The study protocol was approved by The Danish National Committee on Biomedical Research Ethics (protocol no H-2-2010-112).

Trial registration: NCT01700712.

Figures

Figure 1
Figure 1
Mean lactic acid production in plaque suspensions and with addition of L. plantarum 299v and L. reuteri DSM 17938 after fermentation of fructose (a) and xylitol (b). The controls are plaque suspensions without probiotic bacteria. The values shown are μg/mL after 30 min fermentation. ns = not significant.

References

    1. Devine DA, Marsh PD. Prospects for the development of probiotics and prebiotics for oral applications. J Oral Microbiol. 2009;1 doi: 10.3402/jom.v1i0.1949.
    1. Caglar E, Sandalli N, Twetman S, Kavaloglu S, Ergeneli S, Selvi S. Effect of yogurt with Bifidobacterium DN-173 010 on salivary mutans streptococci and lactobacilli in young adults. Acta Odontol Scand. 2005;63:317–320. doi: 10.1080/00016350510020070.
    1. Caglar E, Cildir SK, Ergeneli S, Sandalli N, Twetman S. Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws or tablets. Acta Odontol Scand. 2006;64:314–318. doi: 10.1080/00016350600801709.
    1. Caglar E, Kavaloglu SC, Kuscu OO, Sandalli N, Holgerson PL, Twetman S. Effect of chewing gums containing xylitol or probiotic bacteria on salivary mutans streptococci and lactobacilli. Clin Oral Investig. 2007;11:425–429. doi: 10.1007/s00784-007-0129-9.
    1. Nikawa H, Makihira S, Fukushima H, Nishimura H, Ozaki Y, Ishida K. et al.Lactobacillus reuteri in bovine milk fermented decreases the oral carriage of mutans streptococci. Int J Food Microbiol. 2004;95:219–223. doi: 10.1016/j.ijfoodmicro.2004.03.006.
    1. Ahola AJ, Yli-Knuuttila H, Suomalainen T, Poussa T, Ahlstrom A, Meurman JH. et al.Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors. Arch Oral Biol. 2002;47:799–804. doi: 10.1016/S0003-9969(02)00112-7.
    1. Näse L, Hatakka K, Savilahti E, Saxelin M, Ponka A, Poussa T. et al.Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res. 2001;35:412–420. doi: 10.1159/000047484.
    1. Stecksen-Blicks C, Sjöström I, Twetman S. Effect of long-term consumption of milk supplemented with probiotic lactobacilli and fluoride on dental caries and general health in preschool children: a cluster-randomized study. Caries Res. 2009;43:374–381. doi: 10.1159/000235581.
    1. Petersson LG, Magnusson K, Hakestam U, Baigi A, Twetman S. Reversal of primary root caries lesions after daily intake of milk supplemented with fluoride and probiotic lactobacilli in older adults. Acta Odontol Scand. 2011;69:321–327. doi: 10.3109/00016357.2011.568962.
    1. Montalto M, Vastola M, Marigo L, Covino M, Graziosetto R, Curigliano V. et al.Probiotic treatment increases salivary counts of lactobacilli: a double-blind, randomized, controlled study. Digestion. 2004;69:53–56. doi: 10.1159/000076559.
    1. Runarsson AR. Changes in the oral microflora of occlusal surfaces of teeth with the onset of dental caries. PhD thesis. University of Iceland, Faculty of Odontology; 2009.
    1. Hedberg M, Hasslöf P, Sjöström I, Twetman S, Stecksen-Blicks C. Sugar fermentation in probiotic bacteria–an in vitro study. Oral Microbiol Immunol. 2008;23:482–485. doi: 10.1111/j.1399-302X.2008.00457.x.
    1. Marttinen A, Haukioja A, Karjalainen S, Nylund L, Satokari R, Öhman C. et al.Short-term consumption of probiotic lactobacilli has no effect on acid production of supragingival plaque. Clin Oral Investig. 2011;16:797–803.
    1. Haukioja A, Söderling E, Tenovuo J. Acid production from sugars and sugar alcohols by probiotic lactobacilli and bifidobacteria in vitro. Caries Res. 2008;42:449–453. doi: 10.1159/000163020.
    1. Edgar WM. Sugar substitutes, chewing gum and dental caries–a review. Br Dent J. 1998;184:29–32. doi: 10.1038/sj.bdj.4809535.
    1. Keller MK, Hasslöf P, Stecksén-Blicks C, Twetman S. Co-aggregation and growth inhibition of probiotic lactobacilli and clinical isolates of mutans streptococci: an in vitro study. Acta Odontol Scand. 2011;69:263–268. doi: 10.3109/00016357.2011.554863.
    1. Simark-Mattsson C, Jonsson R, Emilson CG, Roos K. Final pH affects the interference capacity of naturally occurring oral Lactobacillus strains against mutans streptococci. Arch Oral Biol. 2009;54:602–607. doi: 10.1016/j.archoralbio.2009.03.005.
    1. Söderling EM, Marttinen AM, Haukioja AL. Probiotic lactobacilli interfere with Streptococcus mutans biofilm formation in vitro. Curr Microbiol. 2011;62:618–622. doi: 10.1007/s00284-010-9752-9.
    1. Cildir S, Sandalli N, Alp F, Caglar E. A novel delivery system of probiotic drop and its effect on dental caries risk factors in cleft lip/palate children. Cleft Palate Craniofac J. 2011;49:369–72.
    1. Lexner MO, Blomqvist S, Dahlen G, Twetman S. Microbiological profiles in saliva and supragingival plaque from caries-active adolescents before and after a short-term daily intake of milk supplemented with probiotic bacteria - a pilot study. Oral Health Prev Dent. 2010;8:383–388.
    1. de Vrese M, Schrezenmeir J. Probiotics, prebiotics, and synbiotics. Adv Biochem Eng Biotechnol. 2008;111:1–66.
    1. Caglar E, Topcuoglu N, Cildir SK, Sandalli N, Kulekci G. Oral colonization by Lactobacillus reuteri ATCC 55730 after exposure to probiotics. Int J Paediatr Dent. 2009;19:377–381. doi: 10.1111/j.1365-263X.2009.00989.x.
    1. Yli-Knuuttila H, Snäll J, Kari K, Meurman JH. Colonization of Lactobacillus rhamnosus GG in the oral cavity. Oral Microbiol Immunol. 2006;21:129–131. doi: 10.1111/j.1399-302X.2006.00258.x.

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