Sensitivity of Nasopharyngeal Swabs and Saliva for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2

Alainna J Jamal, Mohammad Mozafarihashjin, Eric Coomes, Jeff Powis, Angel X Li, Aimee Paterson, Sofia Anceva-Sami, Shiva Barati, Gloria Crowl, Amna Faheem, Lubna Farooqi, Saman Khan, Karren Prost, Susan Poutanen, Maureen Taylor, Lily Yip, Xi Zoe Zhong, Allison J McGeer, Samira Mubareka, Toronto Invasive Bacterial Diseases Network COVID-19 Investigators, Brenda L Coleman, Danny Chen, Nataly Farshait, Wayne Gold, Christopher E Kandel, Kevin Katz, Robert Kozak, Tony Mazzulli, Matthew Muller, Anne Opavsky, Mario Ostrowski, Agron Plevneshi, Neil Rau, Daniel Ricciuto, David Richardson, David Rose, Valerie Sales, Sharon Walmsley, Alainna J Jamal, Mohammad Mozafarihashjin, Eric Coomes, Jeff Powis, Angel X Li, Aimee Paterson, Sofia Anceva-Sami, Shiva Barati, Gloria Crowl, Amna Faheem, Lubna Farooqi, Saman Khan, Karren Prost, Susan Poutanen, Maureen Taylor, Lily Yip, Xi Zoe Zhong, Allison J McGeer, Samira Mubareka, Toronto Invasive Bacterial Diseases Network COVID-19 Investigators, Brenda L Coleman, Danny Chen, Nataly Farshait, Wayne Gold, Christopher E Kandel, Kevin Katz, Robert Kozak, Tony Mazzulli, Matthew Muller, Anne Opavsky, Mario Ostrowski, Agron Plevneshi, Neil Rau, Daniel Ricciuto, David Richardson, David Rose, Valerie Sales, Sharon Walmsley

Abstract

We enrolled 91 consecutive inpatients with COVID-19 at 6 hospitals in Toronto, Canada, and tested 1 nasopharyngeal swab/saliva sample pair from each patient using real-time RT-PCR for severe acute respiratory syndrome coronavirus 2. Sensitivity was 89% for nasopharyngeal swabs and 72% for saliva (P = .02). Difference in sensitivity was greatest for sample pairs collected later in illness.

Keywords: COVID-19; SARS-CoV-2; nasopharyngeal swab; saliva.

© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

References

    1. BC Centre for Disease Control. COVID-19 care–lab testing. Available at: . Accessed 9 April 2020.
    1. Public Health Ontario. Coronavirus disease 2019 (COVID-19) testing. Available at: . Accessed 9 April 2020.
    1. Yang Y, Yang M, Shen C, et al. Evaluating the accuracy of different respiratory specimens in the laboratory dia gnosis and monitoring the viral shedding of 2019-nCoV infections. medRxiv 2020. Available at: . Accessed 15 April 2020.
    1. Wölfel R, Corman VM, Guggemos W, et al. Virological assessment of hospitalized patients with CO VID-2019. Nature. 2020;581:465–9.
    1. To KK-W, Tsang OT-Y, Yip CC-Y, et al. Consistent detection of 2019 novel coronavirus in saliva. Clin Infect Dis 2020. doi:10.1093/cid/ciaa149.
    1. To KK-W, Tsang OT-Y, Leung W-S, et al. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet Infect Dis 2020;20:565–74.
    1. Wyllie A, Fournier J, Casanovas-Massana A, et al. Saliva is more sensitive for SARS-CoV-2 detection in COVID-19 patients than nasopharyngeal swabs. medRxiv 2020.. Available at: . Accessed 24 April 2020.
    1. Marty FM, Chen K, Verrill KA. How to obtain a nasopharyngeal swab specimen. N Engl J Med 2020;. 382:e76.
    1. Zou L, Ruan F, Huang M, et al. SARS-CoV-2 viral load in upper respiratory specimens of infected patients. N Engl J Med 2020; 382:1177–9. doi:10.1056/NEJMc2001737
    1. Wang W, Xu Y, Gao R, et al. Detection of SARS-CoV-2 in different types of clinical specimens. JAMA 2020; 323:1843–44.
    1. Guo W-L, Jiang Q, Ye F, et al. Effect of throat washings on detection of 2019 novel coronavirus. Clin Infect Dis 2020. doi:10.1093/cid/ciaa416

Source: PubMed

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