Accuracy of saliva and nasopharyngeal sampling for detection of SARS-CoV-2 in community screening: a multicentric cohort study

Solen Kernéis, Caroline Elie, Jacques Fourgeaud, Laure Choupeaux, Séverine Mercier Delarue, Marie-Laure Alby, Pierre Quentin, Juliette Pavie, Patricia Brazille, Marie Laure Néré, Marine Minier, Audrey Gabassi, Aurélien Gibaud, Sébastien Gauthier, Chrystel Leroy, Etienne Voirin-Mathieu, Claire Poyart, Michel Vidaud, Béatrice Parfait, Constance Delaugerre, Jean-Marc Tréluyer, Jérôme LeGoff, Solen Kernéis, Caroline Elie, Jacques Fourgeaud, Laure Choupeaux, Séverine Mercier Delarue, Marie-Laure Alby, Pierre Quentin, Juliette Pavie, Patricia Brazille, Marie Laure Néré, Marine Minier, Audrey Gabassi, Aurélien Gibaud, Sébastien Gauthier, Chrystel Leroy, Etienne Voirin-Mathieu, Claire Poyart, Michel Vidaud, Béatrice Parfait, Constance Delaugerre, Jean-Marc Tréluyer, Jérôme LeGoff

Abstract

Nasopharyngeal sampling for nucleic acid amplification testing (NAAT) is the standard diagnostic test of coronavirus disease 2019. Our objectives were to assess, in real-life conditions, the diagnostic accuracy of a nasopharyngeal point-of-care antigen (Ag) test and of saliva NAAT for detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in ambulatory care. This was a prospective cohort study from 19 October through 18 December 2020 in two community COVID-19 screening centers in Paris, France. Two nasopharyngeal swabs and one saliva sample were simultaneously collected. Diagnostic accuracies of nasopharyngeal Ag testing and of three saliva NAAT methods were assessed as compared to nasopharyngeal NAAT. A total of 1452 ambulatory children and adults were included. Overall, 129/1443 (9%) participants tested positive on nasopharyngeal NAAT (102/564 [18%] in symptomatic and 27/879 [3%] in asymptomatic participants). Sensitivity was 94%, 23%, 96%, and 94% for the three different protocols of saliva NAAT and for the nasopharyngeal Ag test, respectively. Estimates of specificity were above 95% for all methods. Diagnostic accuracy was similar in symptomatic and asymptomatic individuals. Diagnostic accuracy of nasopharyngeal Ag testing and of saliva NAAT is similar to that of nasopharyngeal NAAT, subject to compliance with specific protocols for saliva. Registration number: NCT04578509.

Keywords: Antigen test; Diagnostic performances; Nucleic acid amplification testing; SARS-CoV-2; Saliva.

© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Figures

Fig. 1
Fig. 1
Ct values for nasopharyngeal nucleic acid amplification testing (NAAT) and saliva NAAT (method MGI-2) according to the presence of symptoms and timing of testing after symptoms onset. (1A) nasopharyngeal Ct values, (1B) saliva Ct values (MGI-2)
Fig. 2
Fig. 2
(A, B) Ct values for saliva NAAT (method MGI-2) according to the result of the nasopharyngeal NAAT

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