Plasma Micronutrient Concentrations Are Altered by Antiretroviral Therapy and Lipid-Based Nutrient Supplements in Lactating HIV-Infected Malawian Women

Abstract

Background: Little is known about the influence of antiretroviral therapy with or without micronutrient supplementation on the micronutrient concentrations of HIV-infected lactating women in resource-constrained settings.

Objective: We examined associations of highly active antiretroviral therapy (HAART) and lipid-based nutrient supplements (LNS) with concentrations of selected micronutrients in HIV-infected Malawian women at 24 wk postpartum.

Methods: Plasma micronutrient concentrations were measured in a subsample (n = 690) of Breastfeeding, Antiretrovirals, and Nutrition (BAN) study participants who were randomly assigned at delivery to receive HAART, LNS, HAART+LNS, or no HAART/no LNS (control). HAART consisted of protease inhibitor-based triple therapy. LNS (140 g/d) met energy and micronutrient requirements of lactation. Multivariable linear regression tested the association of HAART and LNS, plus their interaction, with micronutrient concentrations, controlling for season, baseline viral load, and baseline CD4 count.

Results: We found significant HAART by LNS interactions for folate (P = 0.051), vitamin B-12 (P < 0.001), and transferrin receptors (TfRs) (P = 0.085). HAART was associated with lower folate (with LNS: -27%, P < 0.001; without LNS: -12%, P = 0.040) and higher TfR concentrations (with LNS: +14%, P = 0.004; without LNS: +28%, P < 0.001), indicating iron deficiency. LNS increased folate (with HAART: +17%, P = 0.037; without HAART: +39%, P < 0.001) and decreased TfR concentrations (with HAART only: -12%, P = 0.023). HAART was associated with lower vitamin B-12 concentrations only when LNS was present (-18%, P = 0.001), whereas LNS increased vitamin B-12 only when no HAART was present (+27%, P < 0.001). HAART, but not LNS, was associated with higher retinol-binding protein (RBP; +10%, P = 0.007). We detected no association of HAART or LNS with selenium, ferritin, or hemoglobin.

Conclusion: The association of HAART with lower folate, iron deficiency, and higher RBP plus the attenuation of LNS effects on folate and vitamin B-12 when combined with HAART has implications for the health of lactating HIV-infected women taking HAART in prevention of mother-to-child transmission programs. This trial was registered at clinicaltrials.gov as NCT00164736.

Keywords: HIV; highly active antiretroviral therapy; lipid-based nutrient supplements; micronutrient; mothers.

Conflict of interest statement

Author disclosures: VL Flax, LS Adair, LH Allen, S Shahab-Ferdows, D Hampel, CS Chasela, G Tegha, EJ Daza, A Corbett, NL Davis, D Kamwendo, AP Kourtis, DJ Jamieson, and ME Bentley, no conflicts of interest. CM van der Horst received grant support from Abbott Laboratories and GlaxoSmithKline.

© 2015 American Society for Nutrition.

Figures

FIGURE 1

Adjusted geometric mean plasma folate (A), vitamin B-12 (B), and TfR (C) concentrations at 24 wk postpartum in a sample of BAN study mothers who were untreated or given HAART with or without LNS. Values are geometric means and 95% CIs. (A and B) No HAART, no LNS: n = 237; no HAART, LNS: n = 238; NLF, no LNS: n = 43; NLF, LNS: n = 43; LPVr, no LNS: n = 60; LPVr, LNS: n = 67. (C) No HAART, no LNS: n = 237; no HAART, LNS: n = 238; NLF, no LNS: n = 44; NLF, LNS: n = 44; LPVr, no LNS: n = 60; LPVr, LNS: n = 67. Comparisons were made between no LNS and LNS within each drug category (no HAART, NLF, and LPVr) and between NLF or LPVr and no HAART within each LNS category. *,**,***Difference between adjusted means at either end of the bar: *P < 0.05, **P < 0.01, ***P < 0.001. BAN, Breastfeeding, Antiretrovirals, and Nutrition; HAART, highly active antiretroviral therapy; LNS, lipid-based nutrient supplements; LPVr, lopinavir/ritonavir; NLF, nelfinavir; TfR, transferrin receptor.