RNA- temperature-sensitive mutants of vesicular stomatitis virus: L-protein thermosensitivity accounts for transcriptase restriction of group I mutants

D M Hunt, S U Emerson, R R Wagner, D M Hunt, S U Emerson, R R Wagner

Abstract

In vitro transcriptase activity of three group I temperature-sensitive (ts) mutants of vesicular stomatitis virus restricted at 39 C was restored by L-protein fractions derived from wild-type (wt) vesicular stomatitis virion nucleo-capsids. Soluble NS protein from wt nucleocapsids did not reconstitute restricted transcriptions of the group I RNA-ts mutants. NS protein activity, but not L protein activity, was purified from the group I ts mutants; this NS fraction always displayed the wt phenotype in reconstitution assays. Neither the L nor the NS protein was capable of restoring the defective transcriptive activity of the group IV vesicular stomatitis virus mutant ts W16B.

References

    1. J Virol. 1971 Apr;7(4):486-90
    1. J Virol. 1972 Aug;10(2):234-43
    1. J Virol. 1972 Aug;10(2):297-309
    1. J Mol Biol. 1972 Nov 14;71(2):281-91
    1. J Virol. 1972 Nov;10(5):889-95
    1. J Virol. 1972 Dec;10(6):1130-5
    1. J Virol. 1972 Dec;10(6):1228-30
    1. J Virol. 1973 Feb;11(2):279-86
    1. J Gen Virol. 1973 Jun;19(3):295-300
    1. J Virol. 1973 Dec;12(6):1238-52
    1. J Virol. 1973 Dec;12(6):1325-35
    1. J Virol. 1973 Dec;12(6):1395-400
    1. J Virol. 1974 Jan;13(1):28-35
    1. J Virol. 1974 Apr;13(4):922-30
    1. J Virol. 1974 Jul;14(1):139-44
    1. J Virol. 1974 Oct;14(4):765-72
    1. J Immunol. 1963 Jul;91:112-22
    1. J Biol Chem. 1951 Nov;193(1):265-75
    1. J Virol. 1975 Jan;15(1):41-9
    1. J Virol. 1975 Apr;15(4):1004-11
    1. J Virol. 1975 Apr;15(4):1012-9
    1. J Virol. 1975 Jun;15(6):1348-56
    1. Curr Top Microbiol Immunol. 1975;69:85-116
    1. Virology. 1972 Dec;50(3):829-40
    1. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572-6
    1. Virology. 1970 Dec;42(4):946-57
    1. Virology. 1970 Dec;42(4):958-68
    1. J Gen Virol. 1971 May;11(2):81-5
    1. J Virol. 1971 Jul;8(1):56-61

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