Therapeutic Effect of Medical Ozone on Lumbar Disc Herniation

Tong Niu, Chaoliang Lv, Guangkun Yi, Huamin Tang, Cunji Gong, Shizhen Niu, Tong Niu, Chaoliang Lv, Guangkun Yi, Huamin Tang, Cunji Gong, Shizhen Niu

Abstract

BACKGROUND This study aimed to investigate the therapeutic effect of low, medium, and high concentrations of medical ozone on trauma-induced lumbar disc herniation. MATERIAL AND METHODS A total of 80 patients were included and were grouped into a control group, a low medical ozone (20 μg/ml) group, a medium medical ozone (40 μg/ml) group, and a high medical ozone (60 μg/ml) group. The CT scan and enzyme-linked immunosorbent assay (ELISA) were used to detect IL-6 level, SOD activity, IgM, and IgG levels upon admission and at 6 and 12 months after follow-up. The area under the ROC curve (AUC) was calculated for visual analogue scale (VAS) and efficiency rate. RESULTS All patients showed disc retraction at 6- and 12-month follow-up; while patients in the medium medical ozone (40 μg/ml) group showed the greatest disc retraction rate. The IL-6, IgM, IgG, and VAS levels significantly decreased while SOD activity increased among all groups over time (p<0.05). The AUCIL-6, AUCIgG, AUCIgM, and AUCSOD was closest to 1 in the medium medical ozone (40 μg/ml) group compared with other groups (p<0.01), with the highest efficacy at 6 (35%) and 12 (85%) months during follow-up. CONCLUSIONS Low concentrations of medical ozone (20 μg/ml and 40 μg/ml) reduced the serum IL-6, IgG, and IgM expression, presenting as analgesic and anti-inflammatory effects, while high concentrations of medical ozone (60 μg/ml) increased the serum IL-6, IgG, IgM expression, presenting as pain and pro-inflammatory effects. The medical ozone concentration of 40 μg/ml showed the optimal treatment efficacy.

Conflict of interest statement

Conflict of interest

None.

Figures

Figure 1
Figure 1
CT images of Group A, Group B, Group C, and Group D. Upon admission, the intervertebral discs protruded; the intervertebral disc significantly retracted at 6-month follow-up; the intervertebral disc significantly retracted at 12-month follow-up.
Figure 2
Figure 2
ROC curve analysis. (A) ROC curve of Group A. AUCIL-6: 0.557; AUCIgG: 0.582; AUCIgM: 0.504; AUCSOD: 0.314. (B) ROC curve of Group B. AUCIL-6: 0.396; AUCIgG: 0.268; AUCIgM: 0.139; AUCSOD: 0.296. (C) ROC curve of Group C. AUCIL-6: 0.754; AUCIgG: 0.886; AUCIgM: 0.878; AUCSOD: 0.986. (D) ROC curve of Group D. AUCIL-6: 0.270; AUC IgG: 0.238; AUC IgM: 0.468; AUCSOD: 0.393.

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