Preventing friction-induced chondrocyte apoptosis: comparison of human synovial fluid and hylan G-F 20
Kimberly A Waller, Ling X Zhang, Braden C Fleming, Gregory D Jay, Kimberly A Waller, Ling X Zhang, Braden C Fleming, Gregory D Jay
Abstract
Objective: Symptomatic osteoarthritis (OA) is a common painful disease with limited treatment options. A rising number of patients with OA have been treated with intraarticular injections of hyaluronic acid, including the high-molecular-weight hylan G-F 20, which is injected following arthrocentesis. We investigated the effectiveness of hylan G-F 20 to lower coefficient of friction (COF) and prevent chondrocyte apoptosis in vitro.
Methods: A disc-on-disc bovine cartilage bearing was used to measure the static and kinetic COF when lubricated with hylan G-F 20, human synovial fluid (HSF), and phosphate buffered saline (PBS). Following friction testing, we stained paraffin-embedded sections of these cartilage bearings for activated caspase-3, a marker of apoptosis.
Results: Bearings lubricated with hylan G-F 20 had kinetic COF values that were similar to bearings lubricated with PBS, but significantly higher than those lubricated with HSF. There were no significant differences in static COF values in bearings lubricated with hylan G-F 20 as compared to PBS or HSF. However, bearings lubricated with HSF had significantly lower static COF values compared to bearings lubricated with PBS. The mean percentage of caspase-3-positive chondrocytes in the superficial and upper intermediate zones of bearings lubricated with hylan G-F 20 was significantly higher compared to that of bearings lubricated with HSF or unloaded controls, but significantly lower than in those lubricated with PBS.
Conclusion: These findings indicate that joint lubrication may prevent chondrocyte apoptosis by lowering the COF. Further, removal of synovial fluid prior to hylan G-F 20 injection may be detrimental to cartilage health.
Figures
![Figure 1. Schematic diagram of friction and…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3605976/bin/nihms450809f1.jpg)
![Figure 2. COF of bovine osteochondral bearings](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3605976/bin/nihms450809f2.jpg)
Figure 3. Activated caspase-3 in superficial and…
Figure 3. Activated caspase-3 in superficial and upper intermediate zone chondrocytes
Bearings lubricated with hylan…
Figure 4. Bovine osteochondral bearings stained for…
Figure 4. Bovine osteochondral bearings stained for activated caspase-3 (top) and TUNEL (bottom)
Cells positive…
Figure 5. Correlation between static COF (left)…
Figure 5. Correlation between static COF (left) and kinetic COF (right) and percentage of activated…
- Hylan G-F 20 maintains cartilage integrity and decreases osteophyte formation in osteoarthritis through both anabolic and anti-catabolic mechanisms.Li P, Raitcheva D, Hawes M, Moran N, Yu X, Wang F, Matthews GL. Li P, et al. Osteoarthritis Cartilage. 2012 Nov;20(11):1336-46. doi: 10.1016/j.joca.2012.07.004. Epub 2012 Jul 15. Osteoarthritis Cartilage. 2012. PMID: 22809835
- Viscosupplementation with hylan G-F 20: a 26-week controlled trial of efficacy and safety in the osteoarthritic knee.Wobig M, Dickhut A, Maier R, Vetter G. Wobig M, et al. Clin Ther. 1998 May-Jun;20(3):410-23. doi: 10.1016/s0149-2918(98)80052-0. Clin Ther. 1998. PMID: 9663358 Clinical Trial.
- Biodynamic performance of hyaluronic acid versus synovial fluid of the knee in osteoarthritis.Corvelli M, Che B, Saeui C, Singh A, Elisseeff J. Corvelli M, et al. Methods. 2015 Aug;84:90-8. doi: 10.1016/j.ymeth.2015.03.019. Epub 2015 Apr 7. Methods. 2015. PMID: 25858258 Free PMC article.
- The effects of hylan g-f 20 surface modification on gliding of extrasynovial canine tendon grafts in vitro.Kolodzinskyi MN, Zhao C, Sun YL, An KN, Thoreson AR, Amadio PC, Moran SL. Kolodzinskyi MN, et al. J Hand Surg Am. 2013 Feb;38(2):231-6. doi: 10.1016/j.jhsa.2012.11.012. Epub 2013 Jan 5. J Hand Surg Am. 2013. PMID: 23294647 Free PMC article.
- The John Charnley Award Paper. The role of joint fluid in the tribology of total joint arthroplasty.Mazzucco D, Spector M. Mazzucco D, et al. Clin Orthop Relat Res. 2004 Dec;(429):17-32. doi: 10.1097/01.blo.0000150315.47697.b9. Clin Orthop Relat Res. 2004. PMID: 15577461 Review.
- Attachment of cartilage wear particles to the synovium negatively impacts friction properties.Estell EG, Murphy LA, Gangi LR, Shah RP, Ateshian GA, Hung CT. Estell EG, et al. J Biomech. 2021 Oct 11;127:110668. doi: 10.1016/j.jbiomech.2021.110668. Epub 2021 Aug 4. J Biomech. 2021. PMID: 34399243 Free PMC article.
- Molecular Weight of Hyaluronic Acid Has Major Influence on Its Efficacy and Safety for Viscosupplementation in Hip Osteoarthritis: A Systematic Review and Meta-Analysis.Wu YZ, Huang HT, Ho CJ, Shih CL, Chen CH, Cheng TL, Wang YC, Lin SY. Wu YZ, et al. Cartilage. 2021 Dec;13(1_suppl):169S-184S. doi: 10.1177/19476035211021903. Epub 2021 Jun 10. Cartilage. 2021. PMID: 34109828 Free PMC article.
- Arthroscopic irrigation of the bovine stifle joint increases cartilage surface friction and decreases superficial zone lubricin.Teeple E, Karamchedu NP, Larson KM, Zhang L, Badger GJ, Fleming BC, Jay GD. Teeple E, et al. J Biomech. 2016 Sep 6;49(13):3106-3110. doi: 10.1016/j.jbiomech.2016.07.024. Epub 2016 Jul 27. J Biomech. 2016. PMID: 27511596 Free PMC article.
- Reduction of friction by recombinant human proteoglycan 4 in IL-1α stimulated bovine cartilage explants.Larson KM, Zhang L, Elsaid KA, Schmidt TA, Fleming BC, Badger GJ, Jay GD. Larson KM, et al. J Orthop Res. 2017 Mar;35(3):580-589. doi: 10.1002/jor.23367. Epub 2017 Mar 2. J Orthop Res. 2017. PMID: 27411036 Free PMC article.
- Differences among Branded Hyaluronic Acids in Italy, Part 1: Data from In Vitro and Animal Studies and Instructions for Use.Migliore A, Bizzi E, De Lucia O, Delle Sedie A, Bentivegna M, Mahmoud A, Foti C. Migliore A, et al. Clin Med Insights Arthritis Musculoskelet Disord. 2016 May 25;9:89-101. doi: 10.4137/CMAMD.S38857. eCollection 2016. Clin Med Insights Arthritis Musculoskelet Disord. 2016. PMID: 27257398 Free PMC article.
- Research Support, N.I.H., Extramural
- Adult
- Animals
- Apoptosis / drug effects*
- Biocompatible Materials / pharmacology*
- Caspase 3 / analysis
- Cattle
- Cells, Cultured
- Chondrocytes / cytology
- Chondrocytes / drug effects*
- Cytoprotection*
- Friction*
- Humans
- Hyaluronic Acid / analogs & derivatives*
- Hyaluronic Acid / pharmacology
- Lubricants / pharmacology*
- Male
- Synovial Fluid / physiology*
- Biocompatible Materials
- Lubricants
- hylan
- Hyaluronic Acid
- CASP3 protein, human
- Caspase 3
- Full Text Sources
- Other Literature Sources
- Medical
- Research Materials
![Figure 3. Activated caspase-3 in superficial and…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3605976/bin/nihms450809f3.jpg)
Figure 4. Bovine osteochondral bearings stained for…
Figure 4. Bovine osteochondral bearings stained for activated caspase-3 (top) and TUNEL (bottom)
Cells positive…
Figure 5. Correlation between static COF (left)…
Figure 5. Correlation between static COF (left) and kinetic COF (right) and percentage of activated…
![Figure 4. Bovine osteochondral bearings stained for…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3605976/bin/nihms450809f4.jpg)
![Figure 5. Correlation between static COF (left)…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3605976/bin/nihms450809f5.jpg)
Source: PubMed