In vivo Brillouin optical microscopy of the human eye

Giuliano Scarcelli, Seok Hyun Yun, Giuliano Scarcelli, Seok Hyun Yun

Abstract

We report the first Brillouin measurement of the human eye in vivo. We constructed a Brillouin optical scanner safe for human use by employing continuous-wave laser light at 780 nm at a low power of 0.7 mW. With a single scan along the optic axis of the eye, the axial profile of Brillouin frequency shift was obtained with a pixel acquisition time of 0.4 s and axial resolution of about 60 μm, showing the depth-dependent biomechanical properties in the cornea and lens.

Figures

Fig. 1
Fig. 1
Schematic of the Brillouin confocal microscope. OI: Optical isolator. ND: Neutral density filter wheel; BS: beam sampler; SMF: single mode fiber.
Fig. 2
Fig. 2
Laser illumination in the Brillouin scanner. By focusing the beam in the transparent tissue of the anterior chamber (cornea, aqueous humor and lens), the beam is diverging and covers a large area when it hits the retina.
Fig. 3
Fig. 3
Representative Brillouin spectra (anti-Stokes peaks) from a human eye. (a) Corneal stroma. (b) Aqueous humor. (c) Lens nucleus. (d) Vitreous humor. Top panels: raw CCD spectra (average of four frames). Bottom panels: corresponding spectra.
Fig. 4
Fig. 4
In vivo Brillouin measurement of a human eye. (a) Depth profile of the crystalline lens. (b) Depth profile of the anterior chamber. The data are an average of four such scans taken ten minutes apart.

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