- ICH GCP
- Registro de ensayos clínicos de EE. UU.
- Ensayo clínico NCT02519361
Involvement of the Translation Initiation Factors in Resolution of Inflammation in the Elderly Population
The Involvement of the Translation Initiation Factors eIF4E and eIF4G in Resolution of Inflammation in the Elderly Population
Aging cause specific changes in the immune system. Processes like "immunoessence" and "inflammaging" offend the functioning of the immune cells and expose the elderly patient to infections that can lead to morbidity and death.
Protein translation regulation offers a strategic advantage to the immune cells, because it enables rapid activation or termination of synthesis of specific proteins, required for inflammation or its resolution. Translation initiation depends on recruitment of eukaryotic initiation factor "eIF4F" complex.
The aim of the current study is to investigate the involvement of the translation initiation factors (eIF4E and eIF4G) in the process of recovery from acute infection in elderly patient admitted to the internal department with an acute infection.
Descripción general del estudio
Estado
Condiciones
Descripción detallada
- Blood samples of elderly patients diagnosed with acute infection (fever and leukocytosis) will be collected twice - at admittance to Meir hospital and after recovery (24-48 hours without fever and leukocytosis). evidence of infection (chest x ray, urine and blood samples) will be collected. participants will be divided to two age groups: 65-80 yrs and very old patient >85 yrs, and will be compared to younger patients aged 50-65.
- Peripheral blood samples will be separated to subpopulations of lymphocytes, monocytes and polymorphonuclears, and protein will be extracted.
- protein lysates will be separated by SDS-PAGE electrophoresis and immunoblotted for phosphorylated and total eIF4E and eIF4G; their regulators: Mnk 1/2, 4EBP1, mTOR and targets: cyclin D1, c-Myc, survivin, BCL2, VEGFA, etc.
- Paired samples (within each age group) will be analyzed and correlated with clinical response of the patients. The source of the infection will also be considered.
- Comparison between age groups at admittance and upon recovery will be made (Unpaired).
Tipo de estudio
Inscripción (Anticipado)
Contactos y Ubicaciones
Estudio Contacto
- Nombre: rachel heffez ayzenfeld, MD
- Número de teléfono: 09 7472185
- Correo electrónico: rachelhe2@clalit.org.il
Criterios de participación
Criterio de elegibilidad
Edades elegibles para estudiar
Acepta Voluntarios Saludables
Géneros elegibles para el estudio
Método de muestreo
Población de estudio
Descripción
Inclusion Criteria:
- patient aged 50 y and more with:
- fever >38c that started in the 48 hours prior to admission
- leukocytosis >10.0 K/microLiter
- suspected source of infection by symptoms, lab results or imaging
- treatment with antibiotics started in the last 24 hours or is about to be started
Exclusion Criteria:
- patients who cannot sign the informed concent ( dementia)
- patients with active malignancy
- patients who are under chemotherapy
Plan de estudios
¿Cómo está diseñado el estudio?
Detalles de diseño
¿Qué mide el estudio?
Medidas de resultado primarias
Medida de resultado |
Medida Descripción |
Periodo de tiempo |
---|---|---|
Translation initiation factors expression by immunoblotting, in leukocytes sub population
Periodo de tiempo: 1 week
|
Assessment of eIF4E and eIF4G expression by immunoblotting, in leukocytes sub populations under inflamed conditions and upon resolution of acute inflammation, in three groups of age
|
1 week
|
Medidas de resultado secundarias
Medida de resultado |
Medida Descripción |
Periodo de tiempo |
---|---|---|
Translation initiation factors regulators expression by immunoblotting in leukocytes sub population
Periodo de tiempo: 1 week
|
Assessment of eIF4E and eIF4G regulators expression by immunoblotting, in leukocytes sub populations under inflamed conditions and upon resolution of acute inflammation in three age groups
|
1 week
|
Translation initiation factors targets expression by immunoblotting in leukocytes sub population
Periodo de tiempo: 1 week
|
Assessment of eIF4E and eIF4G targets expression by immunoblotting in leukocytes sub populations under inflamed conditions and upon resolution of acute inflammation, in three age groups
|
1 week
|
Colaboradores e Investigadores
Patrocinador
Investigadores
- Investigador principal: Rachel Heffez Ayzenfeld, MD, r.h.ayzenfeld
Fechas de registro del estudio
Fechas importantes del estudio
Inicio del estudio
Finalización primaria (Anticipado)
Finalización del estudio (Anticipado)
Fechas de registro del estudio
Enviado por primera vez
Primero enviado que cumplió con los criterios de control de calidad
Publicado por primera vez (Estimar)
Actualizaciones de registros de estudio
Última actualización publicada (Estimar)
Última actualización enviada que cumplió con los criterios de control de calidad
Última verificación
Más información
Términos relacionados con este estudio
Palabras clave
Términos MeSH relevantes adicionales
Otros números de identificación del estudio
- 0148-15-MMC
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