Unrelated donor α/β T cell- and B cell-depleted HSCT for the treatment of pediatric acute leukemia
Allison Barz Leahy, Yimei Li, Julie-An Talano, Caitlin W Elgarten, Alix E Seif, Yongping Wang, Bryon Johnson, Dimitri S Monos, Stephan Kadauke, Timothy S Olson, Jason Freedman, Lisa Wray, Stephan A Grupp, Nancy Bunin, Allison Barz Leahy, Yimei Li, Julie-An Talano, Caitlin W Elgarten, Alix E Seif, Yongping Wang, Bryon Johnson, Dimitri S Monos, Stephan Kadauke, Timothy S Olson, Jason Freedman, Lisa Wray, Stephan A Grupp, Nancy Bunin
Abstract
Unrelated donor (URD) hematopoietic stem cell transplant (HSCT) is associated with an increased risk of severe graft-versus-host disease (GVHD). TCRαβ/CD19 depletion may reduce this risk, whereas maintaining graft-versus-leukemia. Outcome data with TCRαβ/CD19 depletion generally describe haploidentical donors, with relatively few URDs. We hypothesized that TCRαβ/CD19-depletion would attenuate the risks of GVHD and relapse for URD HSCT. Sixty pediatric and young adult (YA) patients with hematologic malignancies who lacked a matched-related donor were enrolled at 2 large pediatric transplantation centers between October 2014 and September 2019. All patients with acute leukemia had minimal residual disease testing, and DP typing was available for 77%. All patients received myeloablative total body irradiation- or busulfan-based conditioning with no posttransplant immune suppression. Engraftment occurred in 98%. Four-year overall survival was 69% (95% confidence interval [CI], 52%-81%), and leukemia-free survival was 64% (95% CI, 48%-76%), with no difference between lymphoid and myeloid malignancies (P = .6297 and P = .5441, respectively). One patient (1.7%) experienced primary graft failure. Relapse occurred in 11 patients (3-year cumulative incidence, 21%; 95% CI, 11-34), and 8 patients (cumulative incidence, 15%; 95% CI, 6.7-26) experienced nonrelapse mortality. Grade III to IV acute GVHD was seen in 8 patients (13%), and 14 patients (26%) developed chronic GVHD, of which 6 (11%) had extensive disease. Nonpermissive DP mismatch was associated with higher likelihood of acute GVHD (odds ratio, 16.50; 95% CI, 1.67-163.42; P = .0166) but not with the development of chronic GVHD. URD TCRαβ/CD19-depleted peripheral HSCT is a safe and effective approach to transplantation for children/YAs with leukemia. This trial was registered at www.clinicaltrials.gov as #NCT02323867.
© 2022 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.
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References
- Besse K, Maiers M, Confer D, Albrecht M. On modeling human leukocyte antigen-identical sibling match probability for allogeneic hematopoietic cell transplantation: estimating the need for an unrelated donor source. Biol Blood Marrow Transplant. 2016;22(3):410-417.
- Pidala J, Tomblyn M, Nishihori T, et al. . ATG prevents severe acute graft-versus-host disease in mismatched unrelated donor hematopoietic cell transplantation. Biol Blood Marrow Transplant. 2011;17(8):1237-1244.
- Mohty M, Labopin M, Balère ML, et al. . Antithymocyte globulins and chronic graft-vs-host disease after myeloablative allogeneic stem cell transplantation from HLA-matched unrelated donors: a report from the Sociéte Française de Greffe de Moelle et de Thérapie Cellulaire. Leukemia. 2010;24(11):1867-1874.
- Bonifazi F, Rubio M-T, Bacigalupo A, et al. . Rabbit ATG/ATLG in preventing graft-versus-host disease after allogeneic stem cell transplantation: consensus-based recommendations by an international expert panel. Bone Marrow Transplant. 2020;55(6):1093-1102.
- Finke J, Bethge WA, Schmoor C, et al. ; ATG-Fresenius Trial Group . Standard graft-versus-host disease prophylaxis with or without anti-T-cell globulin in haematopoietic cell transplantation from matched unrelated donors: a randomised, open-label, multicentre phase 3 trial. Lancet Oncol. 2009;10(9):855-864.
- Socié G, Schmoor C, Bethge WA, et al. ; ATG-Fresenius Trial Group . Chronic graft-versus-host disease: long-term results from a randomized trial on graft-versus-host disease prophylaxis with or without anti-T-cell globulin ATG-Fresenius. Blood. 2011;117(23):6375-6382.
- Finazzi MC, Boschini C, Craddock C, Rambaldi A, Ward J, Malladi RK. Characteristics of graft-versus-host disease occurring after alemtuzumab-containing allogeneic stem cell transplants: incidence, organ involvement, risk factors and survival. Br J Haematol. 2020;188(4):550-559.
- Berger M, Lanino E, Cesaro S, et al. . Feasibility and outcome of haploidentical hematopoietic stem cell transplantation with post-transplant high-dose cyclophosphamide for children and adolescents with hematologic malignancies: an AIEOP-GITMO Retrospective Multicenter Study. Biol Blood Marrow Transplant. 2016;22(5):902-909.
- Mussetti A, Greco R, Peccatori J, Corradini P. Post-transplant cyclophosphamide, a promising anti-graft versus host disease prophylaxis: where do we stand? Expert Rev Hematol. 2017;10(5):479-492.
- Sharma A, Rastogi N, Chatterjee G, et al. . Haploidentical stem cell transplantation with posttransplant cyclophosphamide for pediatric acute leukemia is safe and effective. J Pediatric Hematology Oncol. 2020.
- Uygun V, Karasu G, Daloğlu H, et al. . Haploidentical hematopoietic stem cell transplantation with post-transplant high-dose cyclophosphamide in high-risk children: a single-center study. Pediatr Transplant. 2019;23(7):e13546.
- Arruda LCM, Gaballa A, Uhlin M. Impact of γδ T cells on clinical outcome of hematopoietic stem cell transplantation: systematic review and meta-analysis. Blood Adv. 2019;3(21):3436-3448.
- Handgretinger R, Schilbach K. The potential role of γδ T cells after allogeneic HCT for leukemia. Blood. 2018;131(10):1063-1072.
- Liu J, Bian Z, Wang X, et al. . Inverse correlation of Vδ2+ T-cell recovery with EBV reactivation after haematopoietic stem cell transplantation. Br J Haematol. 2018;180(2):276-285.
- Ravens S, Schultze-Florey C, Raha S, et al. . Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection [correction published in Nat Immunol. 2018;19:1037]. Nat Immunol. 2017;18(4):393-401.
- de Witte MA, Janssen A, Nijssen K, et al. . αβ T-cell graft depletion for allogeneic HSCT in adults with hematological malignancies. Blood Adv. 2021;5(1):240-249.
- Seif AE, Li Y, Monos DS, et al. . Partially CD3+-depleted unrelated and haploidentical donor peripheral stem cell transplantation has favorable graft-versus-host disease and survival rates in pediatric hematologic malignancy. Biol Blood Marrow Transplant. 2020;26(3):493-501.
- Fleischhauer K, Shaw BE, Gooley T, et al. ; International Histocompatibility Working Group in Hematopoietic Cell Transplantation . Effect of T-cell-epitope matching at HLA-DPB1 in recipients of unrelated-donor haemopoietic-cell transplantation: a retrospective study. Lancet Oncol. 2012;13(4):366-374.
- Crivello P, Zito L, Sizzano F, et al. . The impact of amino acid variability on alloreactivity defines a functional distance predictive of permissive HLA-DPB1 mismatches in hematopoietic stem cell transplantation. Biol Blood Marrow Transplant. 2015;21(2):233-241.
- Center for International Blood & Marrow Transplant Research (CIBMTR). Transplant Essential Data (TED) Manuals CIBMTR Reporting Forms. 2450:Post-TED Q14-18. Last Modified: Dec 22, 2020.
- Przepiorka D, Weisdorf D, Martin P, et al. . 1994 Consensus Conference on acute GVHD grading. Bone Marrow Transpl. 1995;15(6):825-828.
- Lee SJ. Classification systems for chronic graft-versus-host disease. Blood. 2017;129(1):30-37.
- Drobyski WR, Majewski D. Donor γ δ T lymphocytes promote allogeneic engraftment across the major histocompatibility barrier in mice. Blood. 1997;89(3):1100-1109.
- Locatelli F, Merli P, Pagliara D, et al. . Outcome of children with acute leukemia given HLA-haploidentical HSCT after αβ T-cell and B-cell depletion. Blood. 2017;130(5):677-685.
- Zhang M-J, Davies SM, Camitta BM, et al. . Comparison of outcomes after HLA-matched sibling and unrelated donor transplantation for children with high-risk acute lymphoblastic leukemia. Biol Blood Marrow Transplant. 2012;18(8):1204-1210.
- Peters C, Schrappe M, von Stackelberg A, et al. . Stem-cell transplantation in children with acute lymphoblastic leukemia: a prospective international multicenter trial comparing sibling donors with matched unrelated donors: the ALL-SCT-BFM-2003 trial. J Clin Oncol. 2015;33(11):1265-1274.
- Bertaina A, Zecca M, Buldini B, et al. . Unrelated donor vs HLA-haploidentical α/β T-cell- and B-cell-depleted HSCT in children with acute leukemia. Blood. 2018;132(24):2594-2607.
- Shelikhova L, Ilushina M, Shekhovtsova Z, et al. . αβ T cell-depleted haploidentical hematopoietic stem cell transplantation without anti-thymocyte globulin in children with chemorefractory acute myeloid leukemia. Biol Blood Marrow Transplant. 2019;25(5):e179-e182.
- Phelan R, Arora M, Chen M. Current use and outcome of hematopoietic stem cell transplantation: CIBMTR US summary slides, 2020.
- Ringdén O, Remberger M, Runde V, et al. . Peripheral blood stem cell transplantation from unrelated donors: a comparison with marrow transplantation. Blood. 1999;94(2):455-464.
- Meisel R, Laws H-J, Balzer S, et al. . Comparable long-term survival after bone marrow versus peripheral blood progenitor cell transplantation from matched unrelated donors in children with hematologic malignancies. Biol Blood Marrow Transplant. 2007;13(11):1338-1345.
- Kawahara Y, Morimoto A, Inagaki J, et al. . Unrelated cord blood transplantation with myeloablative conditioning for pediatric acute lymphoblastic leukemia in remission: prognostic factors. Bone Marrow Transplant. 2021;56(2):357-367.
- Rocha V, Kabbara N, Ionescu I, Ruggeri A, Purtill D, Gluckman E. Pediatric related and unrelated cord blood transplantation for malignant diseases. Bone Marrow Transplant. 2009;44(10):653-659.
- Styczynski J, Cheung Y-K, Garvin J, et al. . Outcomes of unrelated cord blood transplantation in pediatric recipients. Bone Marrow Transplant. 2004;34(2):129-136.
- Yesilipek MA, Uygun V, Karasu G, Daloglu H, Dincer Z. Haploidentical hematopoietic stem cell transplantation with post-transplant high-dose cyclophosphamide in high-risk children: A single-center study. Pediatr Transplant. 2016;20(3):417-423.
- Arnold DE, MacMath D, Seif AE, et al. . Immune reconstitution following TCRαβ/CD19-depleted hematopoietic cell transplantation for hematologic malignancy in pediatric patients. Transplant Cell Ther. 2021;27(2):169.e1-169.e9.
- Shaw BE, Gooley TA, Malkki M, et al. . The importance of HLA-DPB1 in unrelated donor hematopoietic cell transplantation. Blood. 2007;110(13):4560-4566.
- Leahy AB, Stanley KJ, Myers RM, et al. . Cytogenetic characteristics and outcomes of patients receiving CTL019 CAR T cell therapy. Blood. 2019;134(suppl 1):1464.
Source: PubMed