Diagnosis of Capnocytophaga canimorsus Sepsis by Whole-Genome Next-Generation Sequencing

Maria K Abril, Adam S Barnett, Kara Wegermann, Eric Fountain, Andrew Strand, Benjamin M Heyman, Britton A Blough, Aparna C Swaminathan, Batu Sharma-Kuinkel, Felicia Ruffin, Barbara D Alexander, Chad M McCall, Sylvia F Costa, Murat O Arcasoy, David K Hong, Timothy A Blauwkamp, Michael Kertesz, Vance G Fowler Jr, Bryan D Kraft, Maria K Abril, Adam S Barnett, Kara Wegermann, Eric Fountain, Andrew Strand, Benjamin M Heyman, Britton A Blough, Aparna C Swaminathan, Batu Sharma-Kuinkel, Felicia Ruffin, Barbara D Alexander, Chad M McCall, Sylvia F Costa, Murat O Arcasoy, David K Hong, Timothy A Blauwkamp, Michael Kertesz, Vance G Fowler Jr, Bryan D Kraft

Abstract

We report the case of a 60-year-old man with septic shock due to Capnocytophaga canimorsus that was diagnosed in 24 hours by a novel whole-genome next-generation sequencing assay. This technology shows great promise in identifying fastidious pathogens, and, if validated, it has profound implications for infectious disease diagnosis.

Keywords: high-throughput nucleotide sequencing; microbiological techniques; sepsis/diagnosis.

Figures

Figure 1.
Figure 1.
(A) Timeline of events. Piperacillin-tazobactam (PIPTZ) was administered for a total of 14 days. (B) Wright-Giemsa stain of peripheral blood smear demonstrating blue bacilli (arrows) within neutrophils (original magnification, 1000×). (C) Gram stain of buffy coat demonstrating Gram-negative rods ([GNRs] arrows) within and around neutrophils (original magnification, 1000×). (D) Average alignment coverage over the entire Capnocytophaga canimorsus genome. Overall coverage is 0.71×. (E) Gram-negative rods cultured from blood (original magnification, 1000×) later confirmed by 16S sequencing to be C canimorsus. Abbreviations: ACYV, acyclovir; AMP, ampicillin; CFPM, cefepime; CIPR, ciprofloxacin; DOXY, doxycycline; ICU, intensive care unit; LEVO, levofloxacin; NGS, next-generation sequencing; PCR, polymerase chain reaction; rRNA, ribosomal ribonucleic acid; VANC, vancomycin.

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Source: PubMed

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