Distribution of malassezia species on the scalp in korean seborrheic dermatitis patients

Yang Won Lee, Hee Jin Byun, Beom Joon Kim, Dong Ha Kim, Yun Young Lim, Jin Woong Lee, Myeung Nam Kim, Donghak Kim, Young-Jin Chun, Seog Kyun Mun, Chan Woong Kim, Sung Eun Kim, Jae Sung Hwang, Yang Won Lee, Hee Jin Byun, Beom Joon Kim, Dong Ha Kim, Yun Young Lim, Jin Woong Lee, Myeung Nam Kim, Donghak Kim, Young-Jin Chun, Seog Kyun Mun, Chan Woong Kim, Sung Eun Kim, Jae Sung Hwang

Abstract

Background: Malassezia species play an important role in the pathogenesis of seborrheic dermatitis. In particular, M. restricta and M. globosa are considered to be the predominant organisms in seborrheic dermatitis of Western countries. However, species distribution of Malassezia in seborrheic dermatitis has not been clearly determined yet in Asia.

Objective: To identify the distribution of Malassezia species on the scalp of seborrheic dermatitis patients in Korea using 26S rDNA PCR-RFLP analysis.

Methods: A total of 40 seborrheic dermatitis patients and 100 normal healthy volunteers were included in this study. For the identification of Malassezia species, the scalp scales of the subjects were analyzed by 26S rDNA PCR-RFLP analysis.

Results: The most commonly identified Malassezia species were M. restricta in the seborrheic dermatitis patients, and M. globosa in the normal controls. In the seborrheic dermatitis group, M. restricta was identified in 47.5%, M. globosa in 27.5%, M. furfur in 7.5%, and M. sympodialis in 2.5% of patients. In the healthy control group, M. globosa was identified in 32.0%, M. restricta in 25.0%, M. furfur in 8.0%, M. obtusa in 6.0%, M. slooffiae in 6.0%, and M. sympodialis in 4.0% of subjects.

Conclusion: M. restricta is considered to be the most important Malassezia species in Korean seborrheic dermatitis patients.

Keywords: 26S rDNA PCR-RFLP; Malassezia; Seborrheic dermatitis.

Figures

Fig. 1
Fig. 1
26S rDNA PCR in the six Malassezia standard strains. The size of PCR products were almost 580 bp. Lane M: marker, lane 1: M. furfur, lane 2: M. globosa, lane 3: M. restricta, lane 4: M. obtusa, lane 5: M. sympodialis, lane 6: M. slooffiae.
Fig. 2
Fig. 2
26S rDNA PCR-RFLP pattern in the six Malassezia standard strains. 26S rDNA PCR products were digested with two restriction enzymes. (A) Hha1; all the six standard strains showed different RFLP patterns. (B) BstF51; M. globosa and M. obtusa showed the same RFLP patterns. The other four strains all showed different patterns. Lane M: marker, lane 1: M. furfur, lane 2: M. globosa, lane 3: M. restricta, lane 4: M. obtusa, lane 5: M. sympodialis, lane 6: M. slooffiae.

Source: PubMed

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