Clinical and Functional Significance of TP53 Exon 4-Intron 4 Splice Junction Variants
Emilia M Pinto, Kara N Maxwell, Hadeel Halalsheh, Aaron Phillips, Jacquelyn Powers, Suzanne MacFarland, Michael F Walsh, Kelsey Breen, Maria N Formiga, Richard Kriwacki, Kim E Nichols, Roya Mostafavi, Jinling Wang, Michael R Clay, Carlos Rodriguez-Galindo, Raul C Ribeiro, Gerard P Zambetti, Emilia M Pinto, Kara N Maxwell, Hadeel Halalsheh, Aaron Phillips, Jacquelyn Powers, Suzanne MacFarland, Michael F Walsh, Kelsey Breen, Maria N Formiga, Richard Kriwacki, Kim E Nichols, Roya Mostafavi, Jinling Wang, Michael R Clay, Carlos Rodriguez-Galindo, Raul C Ribeiro, Gerard P Zambetti
Abstract
Germline TP53 splicing variants are uncommon, and their clinical relevance is unknown. However, splice-altering variants at exon 4-intron 4 junctions are relatively enriched in pediatric adrenocortical tumors (ACT). Nevertheless, family histories of cancer compatible with classic Li-Fraumeni syndrome are rarely seen in these patients. We used conventional and in silico assays to determine protein stability, splicing, and transcriptional activity of 10 TP53 variants at exon 4-intron 4 junctions and analyzed their clinical correlates. We reviewed public databases that report the impact of TP53 variants in human cancer and examined individual reports, focusing on family history of cancer. TP53 exon 4-intron 4 junction germline variants were identified in 9 of 75 pediatric ACTs enrolled in the International Pediatric Adrenocortical Tumor Registry and Children's Oncology Group ARAR0332 study. An additional eight independent TP53 variants involving exon 4 splicing were identified in the Pediatric Cancer Genome Project (n = 5,213). These variants resulted in improper expression due to ineffective splicing, protein instability, altered subcellular localization, and loss of function. Clinical case review of carriers of TP53 exon 4-intron 4 junction variants revealed a high incidence of pediatric ACTs and atypical tumor types not consistent with classic Li-Fraumeni syndrome. Germline variants involving TP53 exon 4-intron 4 junctions are frequent in ACT and rare in other pediatric tumors. The collective impact of these germline TP53 variants on the fidelity of splicing, protein structure, and function must be considered in evaluating cancer susceptibility. IMPLICATIONS: Taken together, the data indicate that splice variants at TP53 codon 125 and surrounding bases differentially impacted p53 gene expression and function.
Trial registration: ClinicalTrials.gov NCT00700414 NCT00304070.
Conflict of interest statement
The authors declare no potential conflicts of interest.
©2021 American Association for Cancer Research.
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Source: PubMed