Safety, tolerability, clinical, and joint structural outcomes of a single intra-articular injection of allogeneic mesenchymal precursor cells in patients following anterior cruciate ligament reconstruction: a controlled double-blind randomised trial

Yuanyuan Wang, Andrew Shimmin, Peter Ghosh, Paul Marks, James Linklater, David Connell, Stephen Hall, Donna Skerrett, Silviu Itescu, Flavia M Cicuttini, Yuanyuan Wang, Andrew Shimmin, Peter Ghosh, Paul Marks, James Linklater, David Connell, Stephen Hall, Donna Skerrett, Silviu Itescu, Flavia M Cicuttini

Abstract

Background: Few clinical trials have investigated the safety and efficacy of mesenchymal stem cells for the management of post-traumatic osteoarthritis. The objectives of this pilot study were to determine the safety and tolerability and to explore the efficacy of a single intra-articular injection of allogeneic human mesenchymal precursor cells (MPCs) to improve clinical symptoms and retard joint structural deterioration over 24 months in patients following anterior cruciate ligament (ACL) reconstruction.

Methods: In this phase Ib/IIa, double-blind, active comparator clinical study, 17 patients aged 18-40 years with unilateral ACL reconstruction were randomized (2:1) to receive either a single intra-articular injection of 75 million allogeneic MPCs suspended in hyaluronan (HA) (MPC + HA group) (n = 11) or HA alone (n = 6). Patients were monitored for adverse events. Immunogenicity was evaluated by anti-HLA panel reactive antibodies (PRA) against class I and II HLAs determined by flow cytometry. Pain, function, and quality of life were assessed using the Knee Injury and Osteoarthritis Outcome Score (KOOS) and SF-36v2 scores. Joint space width was measured from radiographs, and tibial cartilage volume and bone area assessed from magnetic resonance imaging (MRI).

Results: Moderate arthralgia and swelling within 24 h following injection that subsided were observed in 4 out of 11 in the MPC + HA group and 0 out of 6 HA controls. No cell-related serious adverse effects were observed. Increases in class I PRA >10% were observed at week 4 in the MPC + HA group that decreased to baseline levels by week 104. Compared with the HA group, MPC + HA-treated patients showed greater improvements in KOOS pain, symptom, activities of daily living, and SF-36 bodily pain scores (p < 0.05). The MPC + HA group had reduced medial and lateral tibiofemoral joint space narrowing (p < 0.05), less tibial bone expansion (0.5% vs 4.0% over 26 weeks, p = 0.02), and a trend towards reduced tibial cartilage volume loss (0.7% vs -4.0% over 26 weeks, p = 0.10) than the HA controls.

Conclusions: Intra-articular administration of a single allogeneic MPC injection following ACL reconstruction was safe, well tolerated, and may improve symptoms and structural outcomes. These findings suggest that MPCs warrant further investigations as they may modulate some of the pathological processes responsible for the development of post-traumatic osteoarthritis following ACL reconstruction.

Trial registration: ClinicalTrials.gov ( NCT01088191 ) registration date: March 11, 2010.

Keywords: Anterior cruciate ligament reconstruction; Cartilage; Function; Mesenchymal precursor cells; Pain; Post-traumatic osteoarthritis; Subchondral bone.

Conflict of interest statement

Ethics approval and consent to participate

Ethics approval was obtained from the Cabrini Human Research Ethics Committee. All participants gave written informed consent.

Consent for publication

Not applicable.

Competing interests

YW and FMC received remuneration from Mesoblast for MRI measurements. PM, JL, and DC received remuneration from Mesoblast for setting up MRI sequences and parameters. AS and SH received remuneration from Mesoblast for the study. PG is a consultant to Mesoblast Ltd. but does not hold stock in the company. DS and SI are employees of Mesoblast, and SI holds stock in the company.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Study flowchart. HA hyaluronan, MPC mesenchymal precursor cell
Fig. 2
Fig. 2
Change from baseline in a–c Knee Injury and Osteoarthritis Outcome Score (KOOS) (pain scores (a), symptom scores (b), and activities of daily living (ADL) scores (c)), and d SF-36 physical component scores over 24 months (mean and 95% confidence interval shown). *p < 0.05 HA hyaluronan, MPC mesenchymal precursor cell
Fig. 3
Fig. 3
Change from baseline in tibiofemoral joint space width over 24 months (medial joint space width (a) and lateral joint space width (b)) (mean and 95% confidence interval shown). HA hyaluronan, MPC mesenchymal precursor cell

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