Persistence of virus-reactive serum immunoglobulin m antibody in confirmed west nile virus encephalitis cases

John T Roehrig, Denis Nash, Beth Maldin, Anne Labowitz, Denise A Martin, Robert S Lanciotti, Grant L Campbell, John T Roehrig, Denis Nash, Beth Maldin, Anne Labowitz, Denise A Martin, Robert S Lanciotti, Grant L Campbell

Abstract

Twenty-nine laboratory-confirmed West Nile virus (WNV encephalitis patients were bled serially so that WNV-reactive immunoglobulin (Ig) M activity could be determined. Of those patients bled, 7 (60%) of 12 had anti-WNV IgM at approximately 500 days after onset. Clinicians should be cautious when interpreting serologic results from early season WNV IgM-positive patients.

Figures

Figure
Figure
Scatterplot of anti–West Nile virus immunoglobulin M positive-to-negative (P/N) values of individual serum specimens over time. Dotted line represents P/N=3.0 cut-off.

References

    1. Anderson JF, Andreadis TG, Vossbrinck CR, Tirrell S, Wakem EM, French RA, et al. Isolation of West Nile virus from mosquitoes, crows, and a Cooper's hawk in Connecticut. Science. 1999;286:2331–3. 10.1126/science.286.5448.2331
    1. Briese T, Jia XY, Huang C, Grady LJ, Lipkin WI. Identification of a Kunjin/West Nile-like flavivirus in brains of patients with New York encephalitis. Lancet. 1999;354:1261–2. 10.1016/S0140-6736(99)04576-6
    1. Jia XY, Briese T, Jordan I, Rambaut A, Chi HC, Mackenzie JS, et al. Genetic analysis of West Nile New York 1999 encephalitis virus. Lancet. 1999;354:1971–2. 10.1016/S0140-6736(99)05384-2
    1. Lanciotti RS, Roehrig JT, Deubel V, Smith J, Parker M, Steele K, et al. Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States. Science. 1999;286:2333–7. 10.1126/science.286.5448.2333
    1. Asnis DS, Conetta R, Teixeira AA, Waldman G, Sampson BA. The West Nile virus outbreak of 1999 in New York: the Flushing Hospital experience. Clin Infect Dis. 2000;30:413–8. 10.1086/313737
    1. Nash D, Mostashari F, Fine A, Miller J, O’Leary D, Murray K, et al. The outbreak of West Nile virus infection in the New York City area in 1999. N Engl J Med. 2001;344:1807–14. 10.1056/NEJM200106143442401
    1. Centers for Disease Control and Prevention. West Nile virus activity–United States, July 31–August 7, 2002, and Louisiana, January 1–August 7, 2002. MMWR Morb Mortal Wkly Rep. 2002;51:681–3.
    1. Petersen LR, Roehrig JT. West Nile virus: a reemerging global pathogen. Emerg Infect Dis. 2001;7:611–4. 10.3201/eid0704.010401
    1. Burke DS, Nisalak A. Detection of Japanese encephalitis virus immunoglobulin M antibodies in serum by antibody capture radioimmunoassay. J Clin Microbiol. 1982;15:353–61.
    1. Burke DS, Nisalak A, Ussery MA. Antibody capture immunoassay detection of Japanese encephalitis virus immunoglobulin M and G antibodies in cerebrospinal fluid. J Clin Microbiol. 1982;16:1034–42.
    1. Monath TP, Nystrom RR, Bailey RE, Calisher CH, Muth DJ. Immunoglobulin M antibody capture enzyme-linked immunosorbent assay for diagnosis of St. Louis encephalitis. J Clin Microbiol. 1984;20:784–90.
    1. Burke DS, Chatiyanonda K, Anandrik S, Nakornsri S, Nisalak A, Hoke CH. Improved surveillance of Japanese encephalitis by detection of virus- specific IgM in desiccated blood specimens. Bull World Health Organ. 1985;63:1037–42.
    1. Burke DS, Nisalak A, Ussery MA, Laorakpongse T, Chantavibul S. Kinetics of IgM and IgG responses to Japanese encephalitis virus in human serum and cerebrospinal fluid. J Infect Dis. 1985;151:1093–9.
    1. Burke DS, Nisalak A, Lorsomrudee W, Ussery MA, Laorpongse T. Virus-specific antibody-producing cells in blood and cerebrospinal fluid in acute Japanese encephalitis. J Med Virol. 1985;17:283–92. 10.1002/jmv.1890170310
    1. Burke DS, Nisalak A, Hoke CH. Field trial of a Japanese encephalitis diagnostic kit. J Med Virol. 1986;18:41–9. 10.1002/jmv.1890180106
    1. Martin DA, Muth DA, Brown T, Johnson AJ, Karabatsos N, Roehrig JT. Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections. J Clin Microbiol. 2000;38:1823–6.
    1. Edelman R, Schneider RJ, Vejjajiva A, Pornpibul R, Voodhikul P. Persistence of virus-specific IgM and clinical recovery after Japanese encephalitis. Am J Trop Med Hyg. 1976;25:733–8.
    1. Han XY, Ren QW, Xu ZY, Tsai TF. Serum and cerebrospinal fluid immunoglobulins M, A, and G in Japanese encephalitis. J Clin Microbiol. 1988;26:976–8.
    1. Tardei G, Ruta S, Chitu V, Rossi C, Tsai TF, Cernescu C. Evaluation of immunoglobulin M (IgM) and IgG enzyme immunoassays in serologic diagnosis of West Nile virus infection. J Clin Microbiol. 2000;38:2232–9.
    1. Johnson AJ, Martin DA, Karabatsos N, Roehrig JT. Detection of anti-arboviral immunoglobulin G by using a monoclonal antibody-based capture enzyme-linked immunosorbent assay. J Clin Microbiol. 2000;38:1827–31.
    1. Martin DA, Biggerstaff BJ, Allen B, Johnson AJ, Lanciotti RS, Roehrig JT. Use of immunoglobulin M cross-reactions in differential diagnosis of human flaviviral encephalitis infections in the United States. Clin Diagn Lab Immunol. 2002;9:544–9.
    1. Mostashari F, Bunning ML, Kitsutani PT, Singer DA, Nash D, Cooper MJ, et al. Epidemic West Nile encephalitis, New York, 1999: results of a household-based seroepidemiological survey. Lancet. 2001;358:261–4. 10.1016/S0140-6736(01)05480-0

Source: PubMed

Patrocinadores y Colaboradores

Condiciones médicas

Intervenciones de drogas

3
Suscribir