Costimulated tumor-infiltrating lymphocytes are a feasible and safe alternative donor cell therapy for relapse after allogeneic stem cell transplantation
Nancy M Hardy, Vicki Fellowes, Jeremy J Rose, Jeanne Odom, Stefania Pittaluga, Seth M Steinberg, Bazetta Blacklock-Schuver, Daniele N Avila, Sarfraz Memon, Roger J Kurlander, Hahn M Khuu, Maryalice Stetler-Stevenson, Esther Mena, Andrew J Dwyer, Bruce L Levine, Carl H June, Ran Reshef, Robert H Vonderheide, Ronald E Gress, Daniel H Fowler, Frances T Hakim, Michael R Bishop, Nancy M Hardy, Vicki Fellowes, Jeremy J Rose, Jeanne Odom, Stefania Pittaluga, Seth M Steinberg, Bazetta Blacklock-Schuver, Daniele N Avila, Sarfraz Memon, Roger J Kurlander, Hahn M Khuu, Maryalice Stetler-Stevenson, Esther Mena, Andrew J Dwyer, Bruce L Levine, Carl H June, Ran Reshef, Robert H Vonderheide, Ronald E Gress, Daniel H Fowler, Frances T Hakim, Michael R Bishop
Abstract
Donor lymphocyte infusion (DLI), a standard relapse treatment after allogeneic stem cell transplantation (AlloSCT), has limited efficacy and often triggers GVHD. We hypothesized that after AlloSCT tumor-infiltrating donor lymphocytes could be costimulated ex vivo to preferentially activate/expand antitumor effectors. We tested the feasibility and safety of costimulated, tumor-derived donor lymphocyte (TDL) infusion in a phase 1 trial. Tumor was resected from 8 patients with B-cell malignancy progression post-AlloSCT; tumor cell suspensions were costimulated with anti-CD3/anti-CD28 Ab-coated magnetic beads and cultured to generate TDL products for each patient. Costimulation yielded increased proportions of T-bet(+)FoxP3(-) type 1 effector donor T cells. A median of 2.04 × 10(7) TDL/kg was infused; TDLs were well tolerated, notably without GVHD. Two transient positron emission tomography (PET) responses and 2 mixed responses were observed in these refractory tumors. TDL are a feasible, tolerable, and novel donor cell therapy alternative for relapse after AlloSCT.
Trial registration: ClinicalTrials.gov NCT00445666.
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Source: PubMed