The Controversy, Challenges, and Potential Benefits of Putative Female Germline Stem Cells Research in Mammals

Zezheng Pan, Mengli Sun, Xia Liang, Jia Li, Fangyue Zhou, Zhisheng Zhong, Yuehui Zheng, Zezheng Pan, Mengli Sun, Xia Liang, Jia Li, Fangyue Zhou, Zhisheng Zhong, Yuehui Zheng

Abstract

The conventional view is that female mammals lose their ability to generate new germ cells after birth. However, in recent years, researchers have successfully isolated and cultured a type of germ cell from postnatal ovaries in a variety of mammalian species that have the abilities of self-proliferation and differentiation into oocytes, and this finding indicates that putative germline stem cells maybe exist in the postnatal mammalian ovaries. Herein, we review the research history and discovery of putative female germline stem cells, the concept that putative germline stem cells exist in the postnatal mammalian ovary, and the research progress, challenge, and application of putative germline stem cells in recent years.

Figures

Figure 1
Figure 1
The isolation and identification of putative FGSCs. (a) H&E stained from histological ovaries sections: putative FGSCs have been considered to be mainly localized in ovarian cortex. (b) IHC results: putative FGSCs were detected by stem cell marker Oct-4 antibody. (c) Putative FGSCs were isolated by primary culture method. Arrows: the freshly isolated putative FGSCs. (d) Arrows: the isolated MVH+ putative FGSCs. (e) Green: the isolated BrdU+ cells. (f) The merger of (d) and (e) by dual immunofluorescence.
Figure 2
Figure 2
The existing challenge of putative FGSCs. ((a) and (b)) The putative FGSCs exact positioning in OSE or ovarian cortex. (c) The “niche” signals regulate PGC to differentiate into putative FGSCs. (d) Is there a putative FGSCs specific marker in cell membrane?

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