Semen quality and sperm DNA damage in relation to urinary bisphenol A among men from an infertility clinic

Abstract

Bisphenol A (BPA) impairs spermatogenesis in animals, but human studies are lacking. We measured urinary BPA concentrations, semen quality, and sperm DNA damage (comet assay) in 190 men recruited through an infertility clinic. BPA was detected in 89% of samples, with a median (interquartile range [IQR]) concentration of 1.3 (0.8-2.5) ng/mL. Urinary BPA concentration was associated with slightly elevated, though not statistically significant, odds for below reference sperm concentration, motility, and morphology. When modeled as continuous dependent variables, an IQR increase in urinary BPA concentration was associated with declines in sperm concentration, motility, and morphology of 23% (95%CI -40%, -0.3%), 7.5% (-17%, +1.5%), and 13% (-26%, -0.1%), respectively, along with a 10% (0.03%, 19%) increase in sperm DNA damage measured as the percentage of DNA in comet tail. In conclusion, urinary BPA may be associated with declined semen quality and increased sperm DNA damage, but confirmatory studies are needed.

Copyright © 2010 Elsevier Inc. All rights reserved.

Figures

Figure 1

Adjusted regression coefficients for a change in semen quality parameter or sperm DNA damage measure associated with increasing quartiles of urinary BPA concentration (n=190): a) sperm concentration (p-value for trend = 0.09); b) sperm motility (p-value for trend = 0.04); c) sperm morphology (p-value for trend = 0.13); d) Tail% (p-value for trend = 0.03). Adjusted for specific gravity, age, BMI, abstinence period, current smoking status, and time of day of urine sample.

Figure 1

Adjusted regression coefficients for a change in semen quality parameter or sperm DNA damage measure associated with increasing quartiles of urinary BPA concentration (n=190): a) sperm concentration (p-value for trend = 0.09); b) sperm motility (p-value for trend = 0.04); c) sperm morphology (p-value for trend = 0.13); d) Tail% (p-value for trend = 0.03). Adjusted for specific gravity, age, BMI, abstinence period, current smoking status, and time of day of urine sample.

Figure 1

Adjusted regression coefficients for a change in semen quality parameter or sperm DNA damage measure associated with increasing quartiles of urinary BPA concentration (n=190): a) sperm concentration (p-value for trend = 0.09); b) sperm motility (p-value for trend = 0.04); c) sperm morphology (p-value for trend = 0.13); d) Tail% (p-value for trend = 0.03). Adjusted for specific gravity, age, BMI, abstinence period, current smoking status, and time of day of urine sample.

Figure 1

Adjusted regression coefficients for a change in semen quality parameter or sperm DNA damage measure associated with increasing quartiles of urinary BPA concentration (n=190): a) sperm concentration (p-value for trend = 0.09); b) sperm motility (p-value for trend = 0.04); c) sperm morphology (p-value for trend = 0.13); d) Tail% (p-value for trend = 0.03). Adjusted for specific gravity, age, BMI, abstinence period, current smoking status, and time of day of urine sample.