Probiotic strain Bacillus subtilis CU1 stimulates immune system of elderly during common infectious disease period: a randomized, double-blind placebo-controlled study

Marie Lefevre, Silvia M Racedo, Gabrielle Ripert, Béatrice Housez, Murielle Cazaubiel, Corinne Maudet, Peter Jüsten, Philippe Marteau, Maria C Urdaci, Marie Lefevre, Silvia M Racedo, Gabrielle Ripert, Béatrice Housez, Murielle Cazaubiel, Corinne Maudet, Peter Jüsten, Philippe Marteau, Maria C Urdaci

Abstract

Background: Bacillus probiotics health benefits have been until now quite poorly studied in the elderly population. This study aimed to assess the effects of Bacillus subtilis CU1 consumption on immune stimulation and resistance to common infectious disease (CID) episodes in healthy free-living seniors.

Results: One hundred subjects aged 60-74 were included in this randomized, double-blind, placebo-controlled, parallel-arms study. Subjects consumed either the placebo or the probiotic (2.10(9) B. subtilis CU1 spores daily) by short periodical courses of 10 days intermittently, alternating 18-day course of break. This scheme was repeated 4 times during the study. Symptoms of gastrointestinal and upper/lower respiratory tract infections were recorded daily by the subjects throughout the study (4 months). Blood, saliva and stool samples were collected in a predefined subset of the first forty-four subjects enrolled in the study. B. subtilis CU1 supplementation did not statistically significantly decrease the mean number of days of reported CID symptoms over the 4-month of study (probiotic group: 5.1 (7.0) d, placebo group: 6.6 (7.3) d, P = 0.2015). However, in the subset of forty-four randomized subjects providing biological samples, we showed that consumption of B. subtilis CU1 significantly increased fecal and salivary secretory IgA concentrations compared to the placebo. A post-hoc analysis on this subset showed a decreased frequency of respiratory infections in the probiotc group compared to the placebo group.

Conclusion: Taken together, our study provides evidence that B. subtilis CU1 supplementation during the winter period may be a safe effective way to stimulate immune responses in elderly subjects.

Keywords: Clinical trial; Common infectious disease; Elderly; Immunostimulation; Probiotics.

Figures

Fig. 1
Fig. 1
Flow chart of subjects
Fig. 2
Fig. 2
Concentrations of secretory IgA in stools. Fecal SIgA concentrations were assessed in subjects from the subset of population (N = 44), at baseline (V1), after 10 days of consumption of study products (V1 + 10 d) and at the end of the study (V3). Values are means, with standard error of means represented by vertical bars. Fecal SIgA concentrations were significantly higher in the probiotic group compared to the placebo group (**P <0.01), and significantly increased in the probiotic group during the study (†† P < 0.01, ††† P < 0.001)
Fig. 3
Fig. 3
Concentrations of secretory IgA in saliva. Salivary SIgA concentrations were assessed in subjects from the subset of population (N = 44), at the end of the study (V3). Values are means, with standard error of means represented by vertical bars. Salivary SIgA concentration was significantly higher in the probiotic group compared to the placebo group (*P <0.05)
Fig. 4
Fig. 4
Concentrations of IFN-gamma in blood. IFN-gamma concentrations were assessed in subjects from the subset of population (N = 44), at baseline (V1), after 10 days of consumption of study products (V1+ 10 d) and at the end of the study (V3). Values are means, with standard error of means represented by vertical bars. IFN-gamma concentrations were significantly increased in the probiotic group between V1 and V1 + 10 d (††P <0.01)
Fig. 5
Fig. 5
Study design. R indicates randomization of the 100 subjects. Blood samples (B), fecal samples (F) and salivary samples (S) concerned a subset of 44 subjects

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