Lab-on-Chip-Based Platform for Fast Molecular Diagnosis of Multidrug-Resistant Tuberculosis
Andrea M Cabibbe, Paolo Miotto, Raquel Moure, Fernando Alcaide, Silke Feuerriegel, Gianni Pozzi, Vladislav Nikolayevskyy, Francis Drobniewski, Stefan Niemann, Klaus Reither, Daniela M Cirillo, TM-REST Consortium, TB-CHILD Consortium, Patrizia Di Pietro, Floriana San Biagio, Enrico Alessi, Tony G Barbuzzi, Silva Tafaj, Elizabetha Bachiyska, Irina Kontsevaya, Yanina Balabanova, Elisa Lazzeri, Joseph Sserunkuma, Francesco Aloi, Martin Nsubuga, Mohamed Sasamalo, Andrea M Cabibbe, Paolo Miotto, Raquel Moure, Fernando Alcaide, Silke Feuerriegel, Gianni Pozzi, Vladislav Nikolayevskyy, Francis Drobniewski, Stefan Niemann, Klaus Reither, Daniela M Cirillo, TM-REST Consortium, TB-CHILD Consortium, Patrizia Di Pietro, Floriana San Biagio, Enrico Alessi, Tony G Barbuzzi, Silva Tafaj, Elizabetha Bachiyska, Irina Kontsevaya, Yanina Balabanova, Elisa Lazzeri, Joseph Sserunkuma, Francesco Aloi, Martin Nsubuga, Mohamed Sasamalo
Abstract
We evaluated the performance of the molecular lab-on-chip-based VerePLEX Biosystem for detection of multidrug-resistant tuberculosis (MDR-TB), obtaining a diagnostic accuracy of more than 97.8% compared to sequencing and MTBDRplus assay for Mycobacterium tuberculosis complex and rifampin and isoniazid resistance detection on clinical isolates and smear-positive specimens. The speed, user-friendly interface, and versatility make it suitable for routine laboratory use.
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Source: PubMed