Tubular expression of KIM-1 does not predict delayed function after transplantation

Abstract

Injured epithelial cells of the proximal tubule upregulate the glycoprotein kidney injury molecule 1 (KIM-1), suggesting its potential as a biomarker of incipient kidney allograft injury. It is unknown whether KIM-1 expression changes in kidney allografts with delayed graft function (DGF), which often follows ischemia-reperfusion injury. Here, we prospectively measured KIM-1 RNA and protein expression in preperfusion biopsies of 30 living- and 85 deceased-donor kidneys and correlated the results with histologic and clinical outcomes after transplantation. We detected KIM-1 expression in 62% of deceased-donor kidneys and only 13% of living-donor kidneys (P < 0.0001). The level of KIM-1 expression before reperfusion correlated inversely with renal function at the time of procurement and correlated directly with the degree of interstitial fibrosis. Surprising, however, we did not detect a significant correlation between KIM-1 staining intensity and the occurrence of DGF. Our findings are consistent with a role for KIM-1 as an early indicator of tubular injury but do not support tissue KIM-1 measurement before transplantation to identify kidneys at risk for DGF.

Figures

Figure 1.

Representative kidney sections from four different donors are based on the KIM-1 staining of proximal tubules (inserts). (A) Score 0.5 defined as focal KIM-1 staining covering a portion of proximal tubules. (B) Score 1, weak but entire luminal staining. (C) Score 2, moderate staining. (D) Score 3, strong staining. Glomeruli and distal nephron tubules stained negatively for KIM-1 in all cases.