Vascular endothelial-cadherin tyrosine phosphorylation in angiogenic and quiescent adult tissues
Nathalie Lambeng, Yann Wallez, Christine Rampon, Francine Cand, Georges Christé, Danielle Gulino-Debrac, Isabelle Vilgrain, Philippe Huber, Nathalie Lambeng, Yann Wallez, Christine Rampon, Francine Cand, Georges Christé, Danielle Gulino-Debrac, Isabelle Vilgrain, Philippe Huber
Abstract
Vascular endothelial-cadherin (VE-cadherin) plays a key role in angiogenesis and in vascular permeability. The regulation of its biological activity may be a central mechanism in normal or pathological angiogenesis. VE-cadherin has been shown to be phosphorylated on tyrosine in vitro under various conditions, including stimulation by VEGF. In the present study, we addressed the question of the existence of a tyrosine phosphorylated form of VE-cadherin in vivo, in correlation with the quiescent versus angiogenic state of adult tissues. Phosphorylated VE-cadherin was detected in mouse lung, uterus, and ovary but not in other tissues unless mice were injected with peroxovanadate to block protein phosphatases. Remarkably, VE-cadherin tyrosine phosphorylation was dramatically increased in uterus and ovary, and not in other organs, during PMSG/hCG-induced angiogenesis. In parallel, we observed an increased association of VE-cadherin with Flk1 (VEGF receptor 2) during hormonal angiogenesis. Additionally, Src kinase was constitutively associated with VE-cadherin in both quiescent and angiogenic tissues and increased phosphorylation of VE-cadherin-associated Src was detected in uterus and ovary after hormonal treatment. Src-VE-cadherin association was detected in cultured endothelial cells, independent of VE-cadherin phosphorylation state and Src activation level. In this model, Src inhibition impaired VEGF-induced VE-cadherin phosphorylation, indicating that VE-cadherin phosphorylation was dependent on Src activation. We conclude that VE-cadherin is a substrate for tyrosine kinases in vivo and that its phosphorylation, together with that of associated Src, is increased by angiogenic stimulation. Physical association between Flk1, Src, and VE-cadherin may thus provide an efficient mechanism for amplification and perpetuation of VEGF-stimulated angiogenic processes.
Figures
![FIGURE 1. VE-cadherin protein expression in mouse…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/2798002/bin/halms433466f1.jpg)
![FIGURE 2. Effect of peroxovanadate treatment on…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/2798002/bin/halms433466f2.jpg)
![FIGURE 3. VE-cadherin tyrosine phosphorylation in hormone-stimulated…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/2798002/bin/halms433466f3.jpg)
![FIGURE 4. Flk1/VE-cadherin protein association in mouse…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/2798002/bin/halms433466f4.jpg)
![FIGURE 5. Src/VE-cadherin association and Src phosphorylation…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/2798002/bin/halms433466f5.jpg)
![Figure 6. Src inhibitors impaired VEGF-induced VE-cadherin…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/2798002/bin/halms433466f6.jpg)
Source: PubMed