RhoA/rho-kinase, nitric oxide and inflammatory response in LIMA during OPCABG with isoflurane preconditioning

Liang Zhang, Cheng-Bin Wang, Bo Li, Duo-Mao Lin, Jun Ma, Liang Zhang, Cheng-Bin Wang, Bo Li, Duo-Mao Lin, Jun Ma

Abstract

Background: Grafting vessel with LIMA to the left anterior descending coronary artery plays a most important role in the long-term prognosis of OPCABG surgery. The aim of this study was to compare the effects of isoflurane preconditioning on miRs and mRNAs levels in the left internal mammary arterie (LIMA) graft with propofol in patients undergoing off-pump coronary artery bypass surgery (OPCABG).

Methods: Patients were randomly assigned to receive either propofol (n = 15), or interrupted isoflurane (n = 15). In group P, propofol administration was continued at 3-5 mg/kg/h intravenous injection for the duration of surgery. Five minutes prior to incision, patients of the isoflurane group (group Iso) received 2 cycles of 1 MAC isoflurane.

Results: miR-221 were significantly lower in group Iso (P < 0 .05). E-selectin mRNA, RhoA mRNA and ROK mRNA were significantly lower at specimens of LIMA in group Iso compared with those in group P patients (P < 0 .05). The expression of NOS3 mRNA was significantly higher in group Iso patients (P < 0 .05).

Conclusion: Our findings provide some insight that prior interrupted isoflurane administration could regulate miR-221, and downstream effectors (mRNAs) and resulted in actual attenuation of inflammation and spasm of LIMA in patients undergoing OPCABG surgery.

Trial registration: NCT No. ( ClinicalTrials.gov ): NCT02678650; Registration date: January 23, 2016.

Keywords: Isoflurane; Left internal mammary artery; OPCABG.

Conflict of interest statement

Ethics approval and consent to participate

As described in the Methods section, the study was approved by Beijing Anzhen Hospital, Capital Medical University. All participants received detailed information about the study and provided written informed consent.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Postoperative release of serum cTnI and CK-MB. (a) Serum cTnI levels at different time points. (b) Serum CK-MB levels at different time points. * P < 0.05, ** P < 0.01, compare with T0; + P < 0.05, ++ P < 0.01, between the groups. T0, 5 min prior to incision; T1, 4 h after surgery; T2, 12 h after surgery; T3, 24 h after surgery; T4, 48 h after surgery
Fig. 2
Fig. 2
Expression change of microRNAs (miRs) in LIMA during OPCABG. Expression change of miR-221 in both groups
Fig. 3
Fig. 3
Expression of NO-associated genes. (a) Expression change of NOS3 mRNA. (b) Expression change of eNOS protein
Fig. 4
Fig. 4
Expression of inflammatory-associated genes. (a, b) Expression change of E-selectin and VCAM-1 mRNA. (c) Expression change of IκB-a mRNA
Fig. 5
Fig. 5
Expression of spasm-associated genes. (a, b) Expression change of RhoA and ROK mRNA. (c) Expression change of RhoA protein

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