A deep profiler's guide to cytometry
Sean C Bendall, Garry P Nolan, Mario Roederer, Pratip K Chattopadhyay, Sean C Bendall, Garry P Nolan, Mario Roederer, Pratip K Chattopadhyay
Abstract
In recent years, advances in technology have provided us with tools to quantify the expression of multiple genes in individual cells. The ability to measure simultaneously multiple genes in the same cell is necessary to resolve the great diversity of cell subsets, as well as to define their function in the host. Fluorescence-based flow cytometry is the benchmark for this; with it, we can quantify 18 proteins per cell, at >10 000 cells/s. Mass cytometry is a new technology that promises to extend these capabilities significantly. Immunophenotyping by mass spectrometry provides the ability to measure >36 proteins at a rate of 1000 cells/s. We review these cytometric technologies, capable of high-content, high-throughput single-cell assays.
Conflict of interest statement
Conflict of Interest Disclosure. M.R. receives royalties on the sale of FlowJo software and Cy7APC fluorescent reagents. G.P.N. owns stock and is a paid consultant with DVS Sciences (CyTOF manufacturer) and is a paid consultant with Becton Dickenson, a purveyor of reagents central to both cytometry platforms.
Published by Elsevier Ltd.
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Source: PubMed