Late preterm birth has direct and indirect effects on infant gut microbiota development during the first six months of life

M Forsgren, E Isolauri, S Salminen, S Rautava, M Forsgren, E Isolauri, S Salminen, S Rautava

Abstract

Aim: Preterm infants display aberrant gut microbial colonisation. We investigated whether the differences in gut microbiota between late preterm and full-term infants results from prematurity or external exposures.

Methods: This study comprised 43 late preterm infants (340/7 -366/7 ) and 75 full-term infants based on faecal samples collected following birth and at two to four weeks and six months of age. We assessed clinically relevant bacteria using quantitative polymerase chain reaction. Logistic regression analyses were performed to determine whether the observed differences in gut microbiota were attributable to prematurity or perinatal exposure.

Results: The prevalence of bifidobacteria differed in the intestinal microbiota of the full-term and late preterm neonates. Differences in the presence of specific species were detected at the age of six months, although the microbiota alterations were most prominent following delivery. As well as prematurity, the mode of birth, intrapartum and neonatal antibiotic exposure, and the duration of breastfeeding had an additional impact on gut microbiota development.

Conclusion: The gut microbiota composition was significantly different between late preterm and full-term infants at least six months after birth. Antibiotic exposure was common in late preterm infants and modulated gut colonisation, but preterm birth also affected gut microbiota development independently.

Keywords: Antibiotics; Caesarean section; Gut colonisation; Intestinal microbiota; Late preterm neonate.

©2017 The Authors. Acta Paediatrica published by John Wiley & Sons Ltd on behalf of Foundation Acta Paediatrica.

Figures

Figure 1
Figure 1
Gut colonisation patterns in full‐term and late preterm infants during the first six months of life. The prevalence of clinically relevant bacteria in full‐term (n = 75) and preterm (n = 43) infant fecal samples collected during first week of life and at two to four weeks and six months of age (*denotes p

References

    1. Romero R, Dey SK, Fisher SJ. Preterm labor: one syndrome, many causes. Science 2014; 15: 760–5.
    1. Stark PL, Lee A. The bacterial colonization of the large bowel of pre‐term low birth weight neonates. J Hyg (Lond) 1982; 89: 59–67.
    1. Rautava S, Luoto R, Salminen S, Isolauri E. Microbial contact during pregnancy, intestinal colonization and human disease. Nat Rev Gastroenterol Hepatol 2012; 9: 565–76.
    1. Kalliomäki M, Kirjavainen P, Eerola E, Kero P, Salminen S, Isolauri E. Distinct patterns of neonatal gut microflora in infants in whom allergy was and was not developing. J Allergy Clin Immunol 2001; 107: 129–34.
    1. Björkstén B, Sepp E, Julge K, Voor T, Mikelsaar M. Allergy development and the intestinal microflora during the first year of life. J Allergy Clin Immunol 2001; 108: 516–20.
    1. Kalliomäki M, Collado MC, Salminen S, Isolauri E. Early differences in fecal microbiota composition in children may predict overweight. Am J Clin Nutr 2008; 87: 534–8.
    1. Karlsson CL, Onnerfält J, Xu J, Molin G, Ahrné S, Thorngren‐Jerneck K. The microbiota of the gut in preschool children with normal and excessive body weight. Obesity (Silver Spring) 2012; 20: 2257–61.
    1. Kalliomäki M, Salminen S, Arvilommi H, Kero P, Koskinen P, Isolauri E. Probiotics in primary prevention of atopic disease. A randomised placebo‐controlled trial. Lancet 2001; 357: 1076–9.
    1. Luoto R, Ruuskanen O, Waris M, Kalliomäki M, Salminen S, Isolauri E. Prebiotic and probiotic supplementation prevents rhinovirus infections in preterm infants. A randomized, placebo‐controlled trial. J Allergy Clin Immunol 2014; 133: 405–13.
    1. Harmsen HJ, Wildeboer‐Veloo AC, Raangs GC, Wagendorp AA, Klijn N, Bindels JG, et al. Analysis of intestinal flora development in breast‐fed and formula‐fed infants by using molecular identification and detection methods. J Pediatr Gastroenterol Nutr 2000; 30: 61–7.
    1. Nylund L, Heilig HG, Salminen S, de Vos WM, Satokari R. Semiautomated extraction of microbial DNA from feces for qPCR and phylogenetic microarray analysis. J Microbiol Methods 2010; 83: 231–5.
    1. Collado MC, Isolauri E, Laitinen K, Salminen S. Distinct composition of gut microbiota during pregnancy in overweight and normal‐weight women. Am J Clin Nutr 2008; 88: 894–9.
    1. Scalabrin DM, Mitmesser SH, Welling GW, Harris CL, Marunycz JD, Walker DC, et al. New prebiotic blend of polydextrose and galacto‐oligosaccharides has bifidogenic effect in young infants. J Pediatr Gastroenterol Nutr 2012; 54: 343–52.
    1. Arboleya S, Sánchez B, Solís G, Fernández N, Suárez M, Hernández‐Barranco AM, et al. Impact of prematurity and perinatal antibiotics on the developing intestinal microbiota: a functional inference study. Int J Mol Sci 2016; 17: 649.
    1. Koren O, Goodrich JK, Cullender TC, Spor A, Laitinen K, Bäckhed HK, et al. Host remodeling of the gut microbiome and metabolic changes during pregnancy. Cell 2012; 3: 470–80.
    1. Dörtbudak O, Eberhardt R, Ulm M, Persson GR. Periodontitis, a marker of risk in pregnancy for preterm birth. J Clin Periodontol 2005; 32: 45–52.
    1. Rougé C, Goldenberg O, Ferraris L, Berger B, Rochat F, Legrand A, et al. Investigation of the intestinal microbiota in preterm infants using different methods. Anaerobe 2010; 16: 362–70.
    1. Arboleya S, Binetti A, Salazar N, Fernández N, Solís G, Hernández‐Barranco A, et al. Establishment and development of intestinal microbiota in preterm neonates. FEMS Microbiol Ecol 2012; 79: 763–72.
    1. Sipola‐Leppänen M, Vääräsmäki M, Tikanmäki M, Matinolli HM, Miettola S, Hovi P, et al. Cardiometabolic risk factors in young adults who were born preterm. Am J Epidemiol 2015; 181: 861–73.

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