Examining multiple sleep behaviors and diurnal salivary cortisol and alpha-amylase: Within- and between-person associations

Abstract

Sleep has been linked to the daily patterns of stress-responsive physiological systems, specifically the hypothalamic-pituitary-adrenal (HPA) axis and autonomic nervous system (ANS). However, extant research examining sleep and diurnal patterns of cortisol, the primary end product of the HPA axis, has primarily focused on sleep duration with limited attention on other facets of sleep. For example, it is not clear how specific aspects of sleep (e.g., sleep quality, sleep duration variability) are related to specific components of diurnal cortisol rhythms. Salivary alpha-amylase (sAA) has been recognized as a surrogate marker of ANS activity, but limited research has explored relations between sleep and sAA diurnal rhythms. The current study utilized an ecological momentary assessment protocol to examine within- and between-person relations between several facets of sleep behavior using multiple methods (e.g., subjective report, actigraphy) and salivary cortisol and sAA. Older adolescents (N=76) provided saliva samples and diary entries five times per day over the course of three days. Sleep was assessed via questionnaire, through daily diaries, and monitored objectively using actigraphy over a four day period. Between-person results revealed that shorter average objective sleep duration and greater sleep duration variability were related to lower levels of waking cortisol and flatter diurnal slopes across the day. Within-person results revealed that on nights when individuals slept for shorter durations than usual they also had lower levels of waking cortisol the next day. Sleep was not related to the cortisol awakening response (CAR) or diurnal patterns of sAA, in either between-person or within-person analyses. However, typical sleep behaviors measured via questionnaire were related to waking levels of sAA. Overall, this study provides a greater understanding of how multiple components of sleep, measured in naturalistic environments, are related to cortisol and sAA diurnal rhythms, and how day-to-day, within-person changes in sleep duration contribute to daily variations in cortisol.

Keywords: Actigraphy; Cortisol; Hypothalamic–pituitary–adrenal axis; Salivary alpha-amylase; Sleep.

Conflict of interest statement

Conflicts of interest

None of the authors (S.A.V., L.D.D.) have any conflicts of interest to declare with respect to this manuscript.

Copyright © 2016 Elsevier Ltd. All rights reserved.

Figures

Fig. 1

Average salivary cortisol and alpha-amylase rhythms across the waking day. Values are aggregated across days (n = 295) and individuals (n = 69). The cortisol and sAA values represent the average level at the following time points across the day: waking, +30 min after waking, approximately 3 and 8 h after waking, and immediately before bedtime.