Smart interface materials integrated with microfluidics for on-demand local capture and release of cells
Umut Atakan Gurkan, Savas Tasoglu, Derya Akkaynak, Oguzhan Avci, Sebnem Unluisler, Serli Canikyan, Noah Maccallum, Utkan Demirci, Umut Atakan Gurkan, Savas Tasoglu, Derya Akkaynak, Oguzhan Avci, Sebnem Unluisler, Serli Canikyan, Noah Maccallum, Utkan Demirci
Abstract
Stimuli responsive, smart interface materials are integrated with microfluidic technologies creating new functions for a broad range of biological and clinical applications by controlling the material and cell interactions. Local capture and on-demand local release of cells are demonstrated with spatial and temporal control in a microfluidic system.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Figures
![Figure 1](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3774603/bin/nihms505392f1.jpg)
![Figure 2](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3774603/bin/nihms505392f2.jpg)
Figure 3
Local release of captured cells…
Figure 3
Local release of captured cells in microchannels. A) Temperature distribution in channels during…
Figure 4
Release of locally captured cells…
Figure 4
Release of locally captured cells in microchannels. A) Temperature change in channels before…
Figure 5
Local capture and release of…
Figure 5
Local capture and release of cells in zones close to the middle of…
Figure 6
Theoretical understanding of local cell…
Figure 6
Theoretical understanding of local cell release in microchannels and comparison to experimental results.…
- An integrated microfluidic system for studying cell-microenvironmental interactions versatilely and dynamically.Liu W, Li L, Wang X, Ren L, Wang X, Wang J, Tu Q, Huang X, Wang J. Liu W, et al. Lab Chip. 2010 Jul 7;10(13):1717-24. doi: 10.1039/c001049a. Epub 2010 Apr 27. Lab Chip. 2010. PMID: 20422110
- Individual blood-cell capture and 2D organization on microarrays.Roupioz Y, Berthet-Duroure N, Leïchlé T, Pourciel JB, Mailley P, Cortes S, Villiers MB, Marche PN, Livache T, Nicu L. Roupioz Y, et al. Small. 2009 Jul;5(13):1493-7. doi: 10.1002/smll.200801941. Small. 2009. PMID: 19306301 No abstract available.
- Integration of plasmonic trapping in a microfluidic environment.Huang L, Maerkl SJ, Martin OJ. Huang L, et al. Opt Express. 2009 Apr 13;17(8):6018-24. doi: 10.1364/oe.17.006018. Opt Express. 2009. PMID: 19365421
- Microfluidic cell sorter (μFCS) for on-chip capture and analysis of single cells.Chung J, Shao H, Reiner T, Issadore D, Weissleder R, Lee H. Chung J, et al. Adv Healthc Mater. 2012 Jul;1(4):432-6. doi: 10.1002/adhm.201200046. Epub 2012 May 2. Adv Healthc Mater. 2012. PMID: 23184773 Free PMC article.
- Current application of micro/nano-interfaces to stimulate and analyze cellular responses.Cho YK, Shin H, Lee SK, Kim T. Cho YK, et al. Ann Biomed Eng. 2010 Jun;38(6):2056-67. doi: 10.1007/s10439-010-9984-7. Epub 2010 Mar 6. Ann Biomed Eng. 2010. PMID: 20213211 Review.
- Plant-inspired TransfOrigami microfluidics.Pan Y, Yang Z, Li C, Hassan SU, Shum HC. Pan Y, et al. Sci Adv. 2022 May 6;8(18):eabo1719. doi: 10.1126/sciadv.abo1719. Epub 2022 May 4. Sci Adv. 2022. PMID: 35507654 Free PMC article.
- Engineering Hydrogel-Based Biomedical Photonics: Design, Fabrication, and Applications.Guimarães CF, Ahmed R, Marques AP, Reis RL, Demirci U. Guimarães CF, et al. Adv Mater. 2021 Jun;33(23):e2006582. doi: 10.1002/adma.202006582. Epub 2021 Apr 30. Adv Mater. 2021. PMID: 33929771 Free PMC article. Review.
- Photonic crystals: emerging biosensors and their promise for point-of-care applications.Inan H, Poyraz M, Inci F, Lifson MA, Baday M, Cunningham BT, Demirci U. Inan H, et al. Chem Soc Rev. 2017 Jan 23;46(2):366-388. doi: 10.1039/c6cs00206d. Chem Soc Rev. 2017. PMID: 27841420 Free PMC article. Review.
- Emerging point-of-care technologies for sickle cell disease screening and monitoring.Alapan Y, Fraiwan A, Kucukal E, Hasan MN, Ung R, Kim M, Odame I, Little JA, Gurkan UA. Alapan Y, et al. Expert Rev Med Devices. 2016 Dec;13(12):1073-1093. doi: 10.1080/17434440.2016.1254038. Epub 2016 Nov 22. Expert Rev Med Devices. 2016. PMID: 27785945 Free PMC article. Review.
- Three-Dimensional Printing Based Hybrid Manufacturing of Microfluidic Devices.Alapan Y, Hasan MN, Shen R, Gurkan UA. Alapan Y, et al. J Nanotechnol Eng Med. 2015 May;6(2):021007. doi: 10.1115/1.4031231. Epub 2015 Sep 29. J Nanotechnol Eng Med. 2015. PMID: 27512530 Free PMC article.
- Research Support, N.I.H., Extramural
- Research Support, U.S. Gov't, Non-P.H.S.
- Acrylic Resins / chemistry*
- Cell Adhesion
- Cell Movement
- Cell Separation / instrumentation*
- Cells, Cultured
- Equipment Design
- Equipment Failure Analysis
- Flow Cytometry / instrumentation*
- Heating / instrumentation*
- Humans
- Lymphocytes / cytology*
- Microfluidic Analytical Techniques / instrumentation*
- Systems Integration
- Acrylic Resins
- poly-N-isopropylacrylamide
- Full Text Sources
- Medical
NCBI Literature Resources
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
![Figure 3](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3774603/bin/nihms505392f3.jpg)
Figure 4
Release of locally captured cells…
Figure 4
Release of locally captured cells in microchannels. A) Temperature change in channels before…
Figure 5
Local capture and release of…
Figure 5
Local capture and release of cells in zones close to the middle of…
Figure 6
Theoretical understanding of local cell…
Figure 6
Theoretical understanding of local cell release in microchannels and comparison to experimental results.…
- An integrated microfluidic system for studying cell-microenvironmental interactions versatilely and dynamically.Liu W, Li L, Wang X, Ren L, Wang X, Wang J, Tu Q, Huang X, Wang J. Liu W, et al. Lab Chip. 2010 Jul 7;10(13):1717-24. doi: 10.1039/c001049a. Epub 2010 Apr 27. Lab Chip. 2010. PMID: 20422110
- Individual blood-cell capture and 2D organization on microarrays.Roupioz Y, Berthet-Duroure N, Leïchlé T, Pourciel JB, Mailley P, Cortes S, Villiers MB, Marche PN, Livache T, Nicu L. Roupioz Y, et al. Small. 2009 Jul;5(13):1493-7. doi: 10.1002/smll.200801941. Small. 2009. PMID: 19306301 No abstract available.
- Integration of plasmonic trapping in a microfluidic environment.Huang L, Maerkl SJ, Martin OJ. Huang L, et al. Opt Express. 2009 Apr 13;17(8):6018-24. doi: 10.1364/oe.17.006018. Opt Express. 2009. PMID: 19365421
- Microfluidic cell sorter (μFCS) for on-chip capture and analysis of single cells.Chung J, Shao H, Reiner T, Issadore D, Weissleder R, Lee H. Chung J, et al. Adv Healthc Mater. 2012 Jul;1(4):432-6. doi: 10.1002/adhm.201200046. Epub 2012 May 2. Adv Healthc Mater. 2012. PMID: 23184773 Free PMC article.
- Current application of micro/nano-interfaces to stimulate and analyze cellular responses.Cho YK, Shin H, Lee SK, Kim T. Cho YK, et al. Ann Biomed Eng. 2010 Jun;38(6):2056-67. doi: 10.1007/s10439-010-9984-7. Epub 2010 Mar 6. Ann Biomed Eng. 2010. PMID: 20213211 Review.
- Plant-inspired TransfOrigami microfluidics.Pan Y, Yang Z, Li C, Hassan SU, Shum HC. Pan Y, et al. Sci Adv. 2022 May 6;8(18):eabo1719. doi: 10.1126/sciadv.abo1719. Epub 2022 May 4. Sci Adv. 2022. PMID: 35507654 Free PMC article.
- Engineering Hydrogel-Based Biomedical Photonics: Design, Fabrication, and Applications.Guimarães CF, Ahmed R, Marques AP, Reis RL, Demirci U. Guimarães CF, et al. Adv Mater. 2021 Jun;33(23):e2006582. doi: 10.1002/adma.202006582. Epub 2021 Apr 30. Adv Mater. 2021. PMID: 33929771 Free PMC article. Review.
- Photonic crystals: emerging biosensors and their promise for point-of-care applications.Inan H, Poyraz M, Inci F, Lifson MA, Baday M, Cunningham BT, Demirci U. Inan H, et al. Chem Soc Rev. 2017 Jan 23;46(2):366-388. doi: 10.1039/c6cs00206d. Chem Soc Rev. 2017. PMID: 27841420 Free PMC article. Review.
- Emerging point-of-care technologies for sickle cell disease screening and monitoring.Alapan Y, Fraiwan A, Kucukal E, Hasan MN, Ung R, Kim M, Odame I, Little JA, Gurkan UA. Alapan Y, et al. Expert Rev Med Devices. 2016 Dec;13(12):1073-1093. doi: 10.1080/17434440.2016.1254038. Epub 2016 Nov 22. Expert Rev Med Devices. 2016. PMID: 27785945 Free PMC article. Review.
- Three-Dimensional Printing Based Hybrid Manufacturing of Microfluidic Devices.Alapan Y, Hasan MN, Shen R, Gurkan UA. Alapan Y, et al. J Nanotechnol Eng Med. 2015 May;6(2):021007. doi: 10.1115/1.4031231. Epub 2015 Sep 29. J Nanotechnol Eng Med. 2015. PMID: 27512530 Free PMC article.
- Research Support, N.I.H., Extramural
- Research Support, U.S. Gov't, Non-P.H.S.
- Acrylic Resins / chemistry*
- Cell Adhesion
- Cell Movement
- Cell Separation / instrumentation*
- Cells, Cultured
- Equipment Design
- Equipment Failure Analysis
- Flow Cytometry / instrumentation*
- Heating / instrumentation*
- Humans
- Lymphocytes / cytology*
- Microfluidic Analytical Techniques / instrumentation*
- Systems Integration
- Acrylic Resins
- poly-N-isopropylacrylamide
- Full Text Sources
- Medical
NCBI Literature Resources
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
![Figure 4](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3774603/bin/nihms505392f4.jpg)
Figure 5
Local capture and release of…
Figure 5
Local capture and release of cells in zones close to the middle of…
Figure 6
Theoretical understanding of local cell…
Figure 6
Theoretical understanding of local cell release in microchannels and comparison to experimental results.…
- An integrated microfluidic system for studying cell-microenvironmental interactions versatilely and dynamically.Liu W, Li L, Wang X, Ren L, Wang X, Wang J, Tu Q, Huang X, Wang J. Liu W, et al. Lab Chip. 2010 Jul 7;10(13):1717-24. doi: 10.1039/c001049a. Epub 2010 Apr 27. Lab Chip. 2010. PMID: 20422110
- Individual blood-cell capture and 2D organization on microarrays.Roupioz Y, Berthet-Duroure N, Leïchlé T, Pourciel JB, Mailley P, Cortes S, Villiers MB, Marche PN, Livache T, Nicu L. Roupioz Y, et al. Small. 2009 Jul;5(13):1493-7. doi: 10.1002/smll.200801941. Small. 2009. PMID: 19306301 No abstract available.
- Integration of plasmonic trapping in a microfluidic environment.Huang L, Maerkl SJ, Martin OJ. Huang L, et al. Opt Express. 2009 Apr 13;17(8):6018-24. doi: 10.1364/oe.17.006018. Opt Express. 2009. PMID: 19365421
- Microfluidic cell sorter (μFCS) for on-chip capture and analysis of single cells.Chung J, Shao H, Reiner T, Issadore D, Weissleder R, Lee H. Chung J, et al. Adv Healthc Mater. 2012 Jul;1(4):432-6. doi: 10.1002/adhm.201200046. Epub 2012 May 2. Adv Healthc Mater. 2012. PMID: 23184773 Free PMC article.
- Current application of micro/nano-interfaces to stimulate and analyze cellular responses.Cho YK, Shin H, Lee SK, Kim T. Cho YK, et al. Ann Biomed Eng. 2010 Jun;38(6):2056-67. doi: 10.1007/s10439-010-9984-7. Epub 2010 Mar 6. Ann Biomed Eng. 2010. PMID: 20213211 Review.
- Plant-inspired TransfOrigami microfluidics.Pan Y, Yang Z, Li C, Hassan SU, Shum HC. Pan Y, et al. Sci Adv. 2022 May 6;8(18):eabo1719. doi: 10.1126/sciadv.abo1719. Epub 2022 May 4. Sci Adv. 2022. PMID: 35507654 Free PMC article.
- Engineering Hydrogel-Based Biomedical Photonics: Design, Fabrication, and Applications.Guimarães CF, Ahmed R, Marques AP, Reis RL, Demirci U. Guimarães CF, et al. Adv Mater. 2021 Jun;33(23):e2006582. doi: 10.1002/adma.202006582. Epub 2021 Apr 30. Adv Mater. 2021. PMID: 33929771 Free PMC article. Review.
- Photonic crystals: emerging biosensors and their promise for point-of-care applications.Inan H, Poyraz M, Inci F, Lifson MA, Baday M, Cunningham BT, Demirci U. Inan H, et al. Chem Soc Rev. 2017 Jan 23;46(2):366-388. doi: 10.1039/c6cs00206d. Chem Soc Rev. 2017. PMID: 27841420 Free PMC article. Review.
- Emerging point-of-care technologies for sickle cell disease screening and monitoring.Alapan Y, Fraiwan A, Kucukal E, Hasan MN, Ung R, Kim M, Odame I, Little JA, Gurkan UA. Alapan Y, et al. Expert Rev Med Devices. 2016 Dec;13(12):1073-1093. doi: 10.1080/17434440.2016.1254038. Epub 2016 Nov 22. Expert Rev Med Devices. 2016. PMID: 27785945 Free PMC article. Review.
- Three-Dimensional Printing Based Hybrid Manufacturing of Microfluidic Devices.Alapan Y, Hasan MN, Shen R, Gurkan UA. Alapan Y, et al. J Nanotechnol Eng Med. 2015 May;6(2):021007. doi: 10.1115/1.4031231. Epub 2015 Sep 29. J Nanotechnol Eng Med. 2015. PMID: 27512530 Free PMC article.
- Research Support, N.I.H., Extramural
- Research Support, U.S. Gov't, Non-P.H.S.
- Acrylic Resins / chemistry*
- Cell Adhesion
- Cell Movement
- Cell Separation / instrumentation*
- Cells, Cultured
- Equipment Design
- Equipment Failure Analysis
- Flow Cytometry / instrumentation*
- Heating / instrumentation*
- Humans
- Lymphocytes / cytology*
- Microfluidic Analytical Techniques / instrumentation*
- Systems Integration
- Acrylic Resins
- poly-N-isopropylacrylamide
- Full Text Sources
- Medical
![Figure 5](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3774603/bin/nihms505392f5.jpg)
Figure 6
Theoretical understanding of local cell…
Figure 6
Theoretical understanding of local cell release in microchannels and comparison to experimental results.…
![Figure 6](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3774603/bin/nihms505392f6.jpg)
Source: PubMed