HIV-1 tropism and liver fibrosis in HIV-HCV co-infected patients
Florence Abravanel, Stéphanie Raymond, Elodie Pambrun, Maria Winnock, Philippe Bonnard, Philippe Sogni, Pascale Trimoulet, François Dabis, Dominique Salmon-Ceron, Jacques Izopet, ANRS CO13 HEPAVIH Study Group, F Dabis, M Winnock, M A Loko, P Sogni, Y Benhamou, P Trimoulet, J Izopet, V Paradis, B Spire, P Carrieri, C Katlama, G Pialoux, M A Valantin, P Bonnard, I Poizot-Martin, B Marchou, E Rosenthal, A Bicart-See, R Djebbar, A Gervais, C Lascoux-Combe, C Goujard, K Lacombe, C Duvivier, D Vittecoq, D Neau, P Morlat, F Bani-Sadr, L Meyer, F Boufassa, S Dominguez, B Autran, A M Roque, C Solas, H Fontaine, L Serfaty, G Chêne, D Costagliola, S Couffin-Cadiergues, D Salmon, P Sogni, B Terris, Z Makhlouf, G Dubost, F Tessier, L Gibault, F Beuvon, E Chambon, T Lazure, A Krivine, C Katlama, M A Valantin, Y Benhamou, S Dominguez, F Charlotte, S Fourati, I Poizot-Martin, O Zaegel, A Ménard, C Tamalet, G Pialoux, P Bonnard, F Bani-Sadr, P Callard, F Bendjaballah, H Assami, B Marchou, L Alric, K Barange, S Metivier, Janick Selves, F Nicot, E Rosenthal, C Pradier, J Haudebourg, M C Saint-Paul, F Rouges, R Djebbar, M Ziol, Y Baazia, M Uzan, A Bicart-See, D Garipuy, P Yéni, A Gervais, H Adle-Biassette, D Séréni, C Lascoux Combe, P Bertheau, J Duclos, P Palmer, P M Girard, K Lacombe, P Campa, D Wendum, P Cervera, J Adam, N Harchi, J F Delfraissy, C Goujard, Y Quertainmont, C Pallier, D Vittecoq, O Lortholary, C Duvivier, S Boucly, D Neau, P Morlat, I Raymond, I Louis, P Bioulac-Sage, P Trimoulet, P Pinson, D Beniken, A-S Ritleng, A Fooladi, M Azar, P Honoré, S Breau, A Joulie, M Mole, C Bolliot, F Touam, F André, G Alexandre, A Mélard, J Baume, S Gillet, J Delaune, L Dequae Merchadou, E Pambrun, A Frosch, J Cohen, G Maradan, C Taieb, F Marcellin, M Mora, C Protopopescu, C Lions, M A Loko, M Winnock, Florence Abravanel, Stéphanie Raymond, Elodie Pambrun, Maria Winnock, Philippe Bonnard, Philippe Sogni, Pascale Trimoulet, François Dabis, Dominique Salmon-Ceron, Jacques Izopet, ANRS CO13 HEPAVIH Study Group, F Dabis, M Winnock, M A Loko, P Sogni, Y Benhamou, P Trimoulet, J Izopet, V Paradis, B Spire, P Carrieri, C Katlama, G Pialoux, M A Valantin, P Bonnard, I Poizot-Martin, B Marchou, E Rosenthal, A Bicart-See, R Djebbar, A Gervais, C Lascoux-Combe, C Goujard, K Lacombe, C Duvivier, D Vittecoq, D Neau, P Morlat, F Bani-Sadr, L Meyer, F Boufassa, S Dominguez, B Autran, A M Roque, C Solas, H Fontaine, L Serfaty, G Chêne, D Costagliola, S Couffin-Cadiergues, D Salmon, P Sogni, B Terris, Z Makhlouf, G Dubost, F Tessier, L Gibault, F Beuvon, E Chambon, T Lazure, A Krivine, C Katlama, M A Valantin, Y Benhamou, S Dominguez, F Charlotte, S Fourati, I Poizot-Martin, O Zaegel, A Ménard, C Tamalet, G Pialoux, P Bonnard, F Bani-Sadr, P Callard, F Bendjaballah, H Assami, B Marchou, L Alric, K Barange, S Metivier, Janick Selves, F Nicot, E Rosenthal, C Pradier, J Haudebourg, M C Saint-Paul, F Rouges, R Djebbar, M Ziol, Y Baazia, M Uzan, A Bicart-See, D Garipuy, P Yéni, A Gervais, H Adle-Biassette, D Séréni, C Lascoux Combe, P Bertheau, J Duclos, P Palmer, P M Girard, K Lacombe, P Campa, D Wendum, P Cervera, J Adam, N Harchi, J F Delfraissy, C Goujard, Y Quertainmont, C Pallier, D Vittecoq, O Lortholary, C Duvivier, S Boucly, D Neau, P Morlat, I Raymond, I Louis, P Bioulac-Sage, P Trimoulet, P Pinson, D Beniken, A-S Ritleng, A Fooladi, M Azar, P Honoré, S Breau, A Joulie, M Mole, C Bolliot, F Touam, F André, G Alexandre, A Mélard, J Baume, S Gillet, J Delaune, L Dequae Merchadou, E Pambrun, A Frosch, J Cohen, G Maradan, C Taieb, F Marcellin, M Mora, C Protopopescu, C Lions, M A Loko, M Winnock
Abstract
Background and aims: Hepatic stellate cells, the major producers of extracellular matrix in the liver, and hepatocytes bear CXCR4 and CCR5, the two main co-receptors for entry of the human immunodeficiency virus (HIV). In vitro studies suggest that HIV-envelope proteins can modulate the replication of hepatitis C virus (HCV) and fibrogenesis. We investigated the influence of HIV tropism on liver fibrosis and the concentration of HCV RNA in HIV-HCV co-infected patients.
Methods: We used a phenotypic assay to assess HIV tropism in 172 HCV-HIV co-infected patients: one group (75 patients) had mild fibrosis (score ≤F2) and the other (97 patients) had severe fibrosis (score >F2). We also assessed the relationship between HIV tropism and HCV RNA concentration in all these patients. We also followed 34 of these patients for 3 years to determine the evolution of HIV tropism and liver fibrosis, estimated by liver stiffness.
Results: Initially, most patients (91.8%) received a potent antiretroviral therapy. CXCR4-using viruses were found in 29% of patients. The only factor associated with a CXCR4-using virus infection in multivariate analysis was the nadir of CD4 cells: <200/mm(3) (OR: 3.94, 95%CI: 1.39-11.14). The median HCV RNA concentrations in patients infected with R5 viruses, those with dual-mixed viruses and those with X4 viruses, were all similar. The prevalence of CXCR4-using viruses in patients with mild fibrosis (≤F2) (31%) and those with severe fibrosis (F3-F4) (28%, p = 0.6) was similar. Longitudinal analyses showed that the presence of CXCR4-using viruses did not increase the likelihood of fibrosis progression, evaluated by measuring liver stiffness.
Conclusions: The presence of CXCR4-using viruses in patients receiving a potent antiretroviral therapy does not influence HCV RNA concentration or liver fibrosis.
Conflict of interest statement
Competing Interests: The ANRS CO13 HEPAVIH cohort is sponsored by Abbott France, Glaxo-Smith-Kline, Roche and Schering-Plough, and INSERM's “Programme cohortes TGIR.” This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.
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