Phage display of functional, full-length human and viral membrane proteins
Sudipta Majumdar, Agnes Hajduczki, Aaron S Mendez, Gregory A Weiss, Sudipta Majumdar, Agnes Hajduczki, Aaron S Mendez, Gregory A Weiss
Abstract
Phage display of protein and peptide libraries offers a powerful technology for the selection and isolation of ligands and receptors. To date, the technique has been considered limited to soluble, non-membrane proteins. We report two examples of phage display of full-length, folded and functional membrane proteins. Consistent display required the recently reported KO7(+) helper phage. The two proteins, full-length caveolin-1 and HIV gp41, display well on the surface of the phage, and maintain their binding activities as shown by in vitro assays.
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Source: PubMed