Multiplex PCR and emerging technologies for the detection of respiratory pathogens
Angela M Caliendo, Angela M Caliendo
Abstract
Molecular methods are becoming more widely used for the detection of respiratory pathogens, in part because of their superior sensitivity, relatively rapid turnaround time, and ability to identify pathogens that are slow growing or difficult to culture. The recent novel H1N1 influenza A pandemic served to underscore how quickly new molecular tests can become available for clinical use. Over the years PCR has been the dominant amplification method. Recently, modifications of this technology have emerged, some of which allow for the rapid detection of multiple pathogens in a single test. This review will focus on emerging multiplex molecular technologies and their clinical utility for the detection of respiratory pathogens.
References
- Barenfanger J, Drake C, Leon N, Mueller T, Troutt T. Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study. J Clin Microbiol. 2000;38:2824–8.
- Woo PC, Chiu SS, Seto WH, Peiris M. Cost-effectiveness of rapid diagnosis of viral respiratory tract infections in pediatric patients. J Clin Microbiol. 1997;35:1579–81.
- Zou S, Han J, Wen L, et al. Human influenza A virus (H5N1) detection by a novel multiplex PCR typing method. J Clin Microbiol. 2007;45:1889–92.
- Brunstein JD, Cline CL, McKinney S, Thomas E. Evidence from multiplex molecular assays for complex multipathogen interactions in acute respiratory infections. J Clin Microbiol. 2008;46:97–102.
- Li H, McCormac MA, Estes RW, et al. Simultaneous detection and high-throughput identification of a panel of RNA viruses causing respiratory tract infections. J Clin Microbiol. 2007;45:2105–9.
- Benson R, Tondella ML, Bhatnagar J, et al. Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens. J Clin Microbiol. 2008;46:2074–7.
- Raymond F, Carbonneau J, Boucher N, et al. Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children. J Clin Microbiol. 2009;47:743–50.
- Beck ET, Jurgens LA, Kehl SC, et al. Development of a rapid automated influenza A, influenza B, and respiratory syncytial virus A/B multiplex real-time RT-PCR assay and its use during the 2009 H1N1 swine-origin influenza virus epidemic in Milwaukee, Wisconsin. J Mol Diagn. 2010;12:74–81.
- Chen et al. PCR and electrospray ionization mass spectrometry (PCR/ESI-MS) for identifying viral respiratory infections [abstract D-2256]. Presented at: 48th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy/46th Annual Meeting of the Infectious Diseases Society of America; 2008.
- Ecker DJ, Sampath R, Blyn LB, et al. Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance. Proc Natl Acad Sci U S A. 2005;102:8012–7.
- Sampath R, Hofstadler SA, Blyn LB, et al. Rapid identification of emerging pathogens: coronavirus. Emerg Infect Dis. 2005;11:373–9.
Source: PubMed