The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

Blerim Kamberi, Donika Bajrami, Miranda Stavileci, Shuhreta Omeragiq, Fatmir Dragidella, Ferit Koçani, Blerim Kamberi, Donika Bajrami, Miranda Stavileci, Shuhreta Omeragiq, Fatmir Dragidella, Ferit Koçani

Abstract

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at P < 0.05. Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

References

    1. Hancock HH, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics. 2001;91(5):579–586.
    1. Siqueira JF, Jr, Rôças IN. Exploiting molecular methods to explore endodontic infections: part 2—Redefining the endodontic microbiota. Journal of Endodontics. 2005;31(7):488–498.
    1. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. Journal of Endodontics. 2006;32(2):93–98.
    1. Sundqvist G, Figdor D, Persson S, Sjögren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics. 1998;85(1):86–93.
    1. Molander A, Reit C, Dahlén G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. International Endodontic Journal. 1998;31(1):1–7.
    1. Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. International Endodontic Journal. 2001;34(6):429–434.
    1. Pinheiro ET, Gomes BPFA, Ferraz CCR, Sousa ELR, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. International Endodontic Journal. 2003;36(1):1–11.
    1. Gomes BPFA, Pinheiro ET, Gadê-Neto CR, et al. Microbiological examination of infected dental root canals. Oral Microbiology and Immunology. 2004;19(2):71–76.
    1. Siqueira JF, Jr, Rĵças IN, Santos SR, Lima KC, Magalhães FA, de Uzeda M. Efficacy of instrumentation techniques and irrigation regimens in reducing the bacterial population within root canals. Journal of Endodontics. 2002;28(3):181–184.
    1. Siqueira JF, Jr, Machado AG, Silveira RM, Lopes HP, De Uzeda M. Evaluation of the effectiveness of sodium hypochlorite used with three irrigation methods in the elimination of Enterococcus faecalis from the root canal, in vitro. International Endodontic Journal. 1997;30(4):279–282.
    1. Torabinejad M, Shabahang S, Bahjri K. Effect of MTAD on postoperative discomfort: a randomized clinical trial. Journal of Endodontics. 2005;31(3):171–176.
    1. Haapasalo M, Udnæs T, Endal U. Persistent, recurrent, and acquired infection of the root canal system post-treatment. Endodontic Topics. 2003;6(1):29–56.
    1. Torabinejad M, Khademi AA, Babagoli J, et al. A new solution for the removal of the smear layer. Journal of Endodontics. 2003;29(3):170–175.
    1. Machnick TK, Torabinejad M, Munoz CA, Shabahang S. Effect of MTAD on flexural strength and modulus of elasticity of dentin. Journal of Endodontics. 2003;29(11):747–750.
    1. Torabinejad M, Shabahang S, Aprecio RM, Kettering JD. The antimicrobial effect of MTAD: an in vitro investigation. Journal of Endodontics. 2003;29(6):400–403.
    1. Shabahang S, Torabinejad M. Effect of MTAD on Enterococcus faecalis-contaminated root canals of extracted human teeth. Journal of Endodontics. 2003;29(9):576–579.
    1. Shabahang S, Pouresmail M, Torabinejad M. In vitro antimicrobial efficacy of MTAD and sodium hypochlorite. Journal of Endodontics. 2003;29(7):450–452.
    1. Zhang W, Torabinejad M, Li Y. Evaluation of cytotoxicity of MTAD using the MTT-tetrazolium method. Journal of Endodontics. 2003;29(10):654–657.
    1. Dunavant TR, Regan JD, Glickman GN, Solomon ES, Honeyman AL. Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilms. Journal of Endodontics. 2006;32(6):527–531.
    1. Kho P, Baumgartner JC. A comparison of the antimicrobial efficacy of NaOCl/Biopure MTAD versus NaOCl/EDTA against Enterococcus faecalis . Journal of Endodontics. 2006;32(7):652–655.
    1. Baumgartner JC, Johal S, Marshall JG. Comparison of the antimicrobial efficacy of 1.3% NaOCl/BioPure MTAD to 5.25% NaOCl/15% EDTA for root canal irrigation. Journal of Endodontics. 2007;33(1):48–51.
    1. Rôças IN, Siqueira JF, Jr, Santos KRN. Association of Enterococcus faecalis with different forms of periradicular diseases. Journal of Endodontics. 2004;30(5):315–320.
    1. Evans M, Davies JK, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. International Endodontic Journal. 2002;35(3):221–228.
    1. Sjögren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. International Endodontic Journal. 1997;30(5):297–306.
    1. Love RM. Enterococcus faecalis-a mechanism for its role in endodontic failure. International Endodontic Journal. 2001;34(5):399–405.
    1. Newberry BM, Shabahang S, Johnson N, Aprecio RM, Torabinejad M. The antimicrobial effect of biopure MTAD on eight strains of Enterococcus faecalis: an in vitro investigation. Journal of Endodontics. 2007;33(11):1352–1354.
    1. Davis JM, Maki J, Bahcall JK. An in vitro comparison of the antimicrobial effects of various endodontic medicaments on Enterococcus faecalis . Journal of Endodontics. 2007;33(5):567–569.
    1. Mohammadi Z, Shahriari S. Residual antibacterial activity of chlorhexidine and MTAD in human root dentin in vitro. Journal of Oral Science. 2008;50(1):63–67.
    1. Portenier I, Waltimo T, Ørstavik D, Haapasalo M. Killing of Enterococcus faecalis by MTAD and chlorhexidine digluconate with or without cetrimide in the presence or absence of dentine powder or BSA. Journal of Endodontics. 2006;32(2):138–141.
    1. Royal MJ, Williamson AE, Drake DR. Comparison of 5.25% sodium hypochlorite, MTAD, and 2% chlorhexidine in the rapid disinfection of polycaprolactone-based root canal filling material. Journal of Endodontics. 2007;33(1):42–44.
    1. Ghoddusi J, Rohani A, Rashed T, Ghaziani P, Akbari M. An evaluation of microbial leakage after using MTAD as a final irrigation. Journal of Endodontics. 2007;33(2):173–176.
    1. Tay FR, Hiraishi N, Schuster GS, et al. Reduction in antimicrobial substantivity of MTAD after initial sodium hypochlorite irrigation. Journal of Endodontics. 2006;32(10):970–975.
    1. Giardino L, Ambu E, Savoldi E, Rimondini R, Cassanelli C, Debbia EA. Comparative evaluation of antimicrobial efficacy of sodium hypochlorite, MTAD, and tetraclean against Enterococcus faecalis biofilm. Journal of Endodontics. 2007;33(7):852–855.
    1. Clegg MS, Vertucci FJ, Walker C, Belanger M, Britto LR. The effect of exposure to irrigant solutions on apical dentin biofilms in vitro. Journal of Endodontics. 2006;32(5):434–437.
    1. Ruff ML, McClanahan SB, Babel BS. In vitro antifungal efficacy of four irrigants as a final rinse. Journal of Endodontics. 2006;32(4):331–333.
    1. Krause TA, Liewehr FR, Hahn CL. The antimicrobial effect of MTAD, sodium hypochlorite, doxycycline, and citric acid on Enterococcus faecalis . Journal of Endodontics. 2007;33(1):28–30.

Source: PubMed

Sponsor e collaboratori

Condizioni mediche

Interventi farmacologici

3
Sottoscrivi