Adult cardiac-resident MSC-like stem cells with a proepicardial origin
James J H Chong, Vashe Chandrakanthan, Munira Xaymardan, Naisana S Asli, Joan Li, Ishtiaq Ahmed, Corey Heffernan, Mary K Menon, Christopher J Scarlett, Amirsalar Rashidianfar, Christine Biben, Hans Zoellner, Emily K Colvin, John E Pimanda, Andrew V Biankin, Bin Zhou, William T Pu, Owen W J Prall, Richard P Harvey, James J H Chong, Vashe Chandrakanthan, Munira Xaymardan, Naisana S Asli, Joan Li, Ishtiaq Ahmed, Corey Heffernan, Mary K Menon, Christopher J Scarlett, Amirsalar Rashidianfar, Christine Biben, Hans Zoellner, Emily K Colvin, John E Pimanda, Andrew V Biankin, Bin Zhou, William T Pu, Owen W J Prall, Richard P Harvey
Abstract
Colony-forming units - fibroblast (CFU-Fs), analogous to those giving rise to bone marrow (BM) mesenchymal stem cells (MSCs), are present in many organs, although the relationship between BM and organ-specific CFU-Fs in homeostasis and tissue repair is unknown. Here we describe a population of adult cardiac-resident CFU-Fs (cCFU-Fs) that occupy a perivascular, adventitial niche and show broad trans-germ layer potency in vitro and in vivo. CRE lineage tracing and embryo analysis demonstrated a proepicardial origin for cCFU-Fs. Furthermore, in BM transplantation chimeras, we found no interchange between BM and cCFU-Fs after aging, myocardial infarction, or BM stem cell mobilization. BM and cardiac and aortic CFU-Fs had distinct CRE lineage signatures, indicating that they arise from different progenitor beds during development. These diverse origins for CFU-Fs suggest an underlying basis for differentiation biases seen in different CFU-F populations, and could also influence their capacity for participating in tissue repair.
Copyright © 2011 Elsevier Inc. All rights reserved.
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Source: PubMed