Effects of Arabinoxylan and Resistant Starch on Intestinal Microbiota and Short-Chain Fatty Acids in Subjects with Metabolic Syndrome: A Randomised Crossover Study

Abstract

Recently, the intestinal microbiota has been emphasised as an important contributor to the development of metabolic syndrome. Dietary fibre may exert beneficial effects through modulation of the intestinal microbiota and metabolic end products. We investigated the effects of a diet enriched with two different dietary fibres, arabinoxylan and resistant starch type 2, on the gut microbiome and faecal short-chain fatty acids. Nineteen adults with metabolic syndrome completed this randomised crossover study with two 4-week interventions of a diet enriched with arabinoxylan and resistant starch and a low-fibre Western-style diet. Faecal samples were collected before and at the end of the interventions for fermentative end-product analysis and 16S ribosomal RNA bacterial gene amplification for identification of bacterial taxa. Faecal carbohydrate residues were used to verify compliance. The diet enriched with arabinoxylan and resistant starch resulted in significant reductions in the total species diversity of the faecal-associated intestinal microbiota but also increased the heterogeneity of bacterial communities both between and within subjects. The proportion of Bifidobacterium was increased by arabinoxylan and resistant starch consumption (P<0.001), whereas the proportions of certain bacterial genera associated with dysbiotic intestinal communities were reduced. Furthermore, the total short-chain fatty acids (P<0.01), acetate (P<0.01) and butyrate concentrations (P<0.01) were higher by the end of the diet enriched with arabinoxylan and resistant starch compared with those resulting from the Western-style diet. The concentrations of isobutyrate (P = 0.05) and isovalerate (P = 0.03) decreased in response to the arabinoxylan and resistant starch enriched diet, indicating reduced protein fermentation. In conclusion, arabinoxylan and resistant starch intake changes the microbiome and short-chain fatty acid compositions, with potential beneficial effects on colonic health and metabolic syndrome.

Trial registration: ClinicalTrials.gov NCT01618526.

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1. Subject flow.

Fig 2. A Principal coordinate analysis of weighted UniFrac values between faecal microbial communities.

The green and red arrows represent bacterial communities before and at the end of healthy-carbohydrate diet (HCD) and Western-style diet (WSD) consumption, respectively. The dotted lines connect the microbiota present during consumption of the two baseline diets for each individual. B Box plot of the weighted UniFrac distances. Intra-individual microbiota distances are shown on the left, and inter-individual distances are shown on the right. * P< 0.05, **** P< 0.0001 (Wilcoxon signed-rank test).

Fig 3. Box plot of the log2-transformed fold-changes in the relative abundances of taxa in the faeces collected at the end of consumption of the healthy-carbohydrate diet (HCD) compared with that of the Western-style diet (WSD).

Only taxa that were significantly affected by diet (P < 0.05) are shown. Family, order, and genus distinctions are represented by (f), (o), and (g), respectively. The boxes and text are coloured according to the phyla as follows: Actinobacteria (blue), Bacteroidetes (green), Firmicutes (purple), and Proteobacteria (red). * P < 0.05, ** P < 0.01, *** P < 0.001 (Wilcoxon signed-rank test).

Fig 4. Faecal short-chain fatty acid (SCFA) and branched-chain fatty acid (BCFA) concentrations.

A, C Differences between Western-style diet (WSD) and healthy-carbohydrate diet (HCD) concentrations are indicated by NS; non significant, *P < 0.05 and **P< 0.01 (ANOVA for repeated measurements using subject, diet, period and baseline values as covariates). The values are the medians with interquartile ranges (n = 19) as the data were log-transformed. B Individual faecal butyrate concentration before (Pre-HCD) and after HCD. The solid line represents the median pre-HCD concentration and the dashed lines indicate the interquartile range of the pre-HCD concentrations.