Comparative efficacy of a hydroxyapatite and a fluoride toothpaste for prevention and remineralization of dental caries in children

Bennett T Amaechi, Parveez Ahamed AbdulAzees, Dina Ossama Alshareif, Marina Adel Shehata, Patrícia Paula de Carvalho Sampaio Lima, Azadeh Abdollahi, Parisa Samadi Kalkhorani, Veronica Evans, Bennett T Amaechi, Parveez Ahamed AbdulAzees, Dina Ossama Alshareif, Marina Adel Shehata, Patrícia Paula de Carvalho Sampaio Lima, Azadeh Abdollahi, Parisa Samadi Kalkhorani, Veronica Evans

Abstract

Objective: This in situ study compared the effectiveness of two toothpastes containing hydroxyapatite or 500 ppm fluoride in promoting remineralization and inhibiting caries development.

Materials and methods: Two enamel blocks (human primary teeth), one sound and one with artificially-produced caries lesion, were exposed to toothpaste containing either 10% hydroxyapatite or 500 ppm F- (amine fluoride) via intra-oral appliance worn by 30 adults in two-arm double blind randomized crossover study lasting 14 days per arm (ClinicalTrials.gov: NCT03681340). Baseline and post-test mineral loss and lesion depth (LD) were quantified using microradiography. One-sided t-test of one group mean was used for intragroup comparison (baseline vs. post-test), while two-sided t-test of two independent means was used to compare the two toothpaste groups.

Results: Pairwise comparison (baseline vs. test) indicated significant (p < 0.0001) remineralization and LD reduction by either toothpaste; however, when compared against each other, there was no statistically significant difference in remineralization or LD reduction between the two toothpastes. No demineralization could be observed in sound enamel blocks exposed to either toothpaste. While F- induced lesion surface lamination, HAP produced a more homogenous lesion remineralization.

Conclusions: 10% hydroxyapatite achieved comparable efficacy with 500 ppm F- in remineralizing initial caries and preventing demineralization. Thus the HAP toothpaste is confirmed to be equal to the fluoride toothpaste in this study.

Keywords: Oral diseases; Paediatric dentistry.

Conflict of interest statement

Competing interestsThe authors declare no competing interests.

© The Author(s) 2019.

Figures

Fig. 1
Fig. 1
Flow Diagram detailing the stepwise methodology. This is a crossover study so the 30 completers received the two intervention in a crossover design as phase I and II.
Fig. 2
Fig. 2
Representative microradiographic images of sound tooth tissue before (a) and after (b) intra-oral exposure for demineralization while the research subject is using Karex Kid’s toothpaste (10% HAP microclusters).
Fig. 3
Fig. 3
Representative microradiographic images of sound tooth tissue before (a) and after (b) intra-oral exposure for demineralization while the research subject is using Elmex Kid’s toothpaste (500 ppm fluoride as AMF).
Fig. 4
Fig. 4
Representative microradiographic images of enamel subsurface lesions (Initial caries lesions), before (a) and after (b) in situ remineralization via treatment with Karex Kid’s toothpaste (10% HAP microclusters).
Fig. 5
Fig. 5
Representative microradiographic images of enamel subsurface lesions (Initial caries lesions), before (a) and after (b) in situ remineralization via treatment with Elmex Kid’s toothpaste (500 ppm fluoride as AMF).

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