Dynamic Connectivity Under Metabolic Constraints
The investigators are studying the impact of insulin resistance on the acceleration of brain aging and testing whether increased neuron insulin resistance can be counteracted by utilization of alternate metabolic pathways (e.g., ketones rather than glucose). This study has three Arms, which together provide synergistic data. For all three Arms, subjects are tested in a within-subjects design that consists of 2-3 testing sessions, 1-14 days apart, and counter-balanced for order. Impact of fuel (glucose in one session, ketones in the other) on brain metabolism and associated functioning is measured during each session. For Arms 1-2, the primary experimental measure is functional magnetic resonance imaging (fMRI), which is used to trace the self-organization of functional networks following changes in energy supply and demand. Arm 1 tests the impact of endogenous ketones produced by switching to a low carbohydrate diet, while Arm 2 tests the impact of exogenous ketones consumed as a nutritional supplement. For Arm 3, simultaneous magnetic resonance spectroscopy/positron-emission tomography (MR/PET) is used to quantify the impact of exogenous ketones on production of glutamate and GABA, key neurotransmitters.
Subjects will be given the option to participate in more than one of the Arms, but doing so is not expected nor required.
Prior to scans, subjects will receive a clinician-administered History and Physical (H&P), which includes vital signs, an oral glucose tolerance test (OGTT), and the comprehensive metabolic blood panel. These will be used to assess diabetes, kidney disease, and electrolytes. If subjects pass screening, they will be provided the option to participate in one or more Arms, which include neuroimaging. To provide a quantitative measure of time-varying metabolic activity throughout the scan, based upon quantitative models of glucose and ketone regulation, as well as to be able to implement safety stopping rules (see below), the investigators will obtain pin-prick blood samples three times: prior to the scan, following consumption of the glucose or ketone drink, and following completion of the scan. To assess effects of increased metabolic demand, the investigators measure brain response to cognitive load, transitioning from resting-state to spatial reasoning through a spatial navigation video task. To assess effects of increased metabolic supply, the investigators measure brain response to glucose or ketone bolus.
調査の概要
研究の種類
入学 (予想される)
段階
- フェーズ 4
連絡先と場所
研究場所
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Massachusetts
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Charlestown、Massachusetts、アメリカ、02129
- 募集
- Martinos Center for Biomedical Research, Building 149
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New York
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Stony Brook、New York、アメリカ、11794
- 募集
- Bioengineering Building , Stony Brook University
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主任研究者:
- Lilianne Mujica-Parodi, PhD
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参加基準
適格基準
就学可能な年齢
健康ボランティアの受け入れ
受講資格のある性別
説明
Exclusion Criteria:
- claustrophobia
- history of neurological disease, heart attack, stroke, kidney disease, or myxedema
- chronic usage of alcohol
- current usage of psychotropic medication
- Type 1 diabetes mellitus
- Regular consumption of insulin, Metformin® or other medications (statins, NSAIDs, beta-blockers, glucocorticoids) that affect glucose and/or insulin utilization.
- difficulty swallowing
- pregnancy
- breastfeeding
- For PET: research imaging-related radiation exposure that exceeds current MGH Radiology Radiation Safety Commitee guidelines.
Inclusion Criteria:
- BMI < 30
- MRI compatible
- For PET with Optional 150 ml Blood Sampling Only: Must weigh at least 110 lbs to minimize risks per PHRC guidelines.
- 20/20 vision or correctable to 20/20 with contact lenses
研究計画
研究はどのように設計されていますか?
デザインの詳細
- 主な目的:基礎科学
- 割り当て:ランダム化
- 介入モデル:クロスオーバー割り当て
- マスキング:独身
武器と介入
参加者グループ / アーム |
介入・治療 |
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実験的:Arm 1: Metabolic Manipulation via Diet fMRI
All subjects are tested three times, each in a different diet-induced metabolic state: glycolytic (glucose burning), fasting (8 hours no food), and ketotic (fat burning).
While having their brains scanned with MRI, subjects are initially tested at rest, and then perform a task.
Midway through the session, subjects are removed from the scanner and drink up to 75g glucose.
Data analyses quantify network reorganization in response to changing energy constraints (i.e., cognitive demand, fuel).
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Supplement administered mid-scan.
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実験的:Arm 2: Metabolic Manipulation via Ketone Supplement fMRI
All subjects are tested twice, both times in a fasting condition (8 hours no food, unrestricted water).
While having their brains scanned with MRI, subjects are initially tested at rest, and then perform a task.
Midway through the session, subjects are removed from the scanner and drink either of two fuel sources.
In the ketotic (ketone burning) session they will drink a ketone sports drink dosed at 395mg/kg.
During the glycolytic (glucose burning) session the same subjects will drink a bolus of glucose, calorie-matched to the ketones.
Data analyses quantify network reorganization in response to changing energy constraints (i.e., cognitive demand, fuel).
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Supplement administered mid-scan.
Supplement administered mid-scan.
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実験的:Arm 3: Metabolic Manipulation via Ketone Supplement MR/PET
All subjects are tested twice, both times in a fasting condition (8 hours no food, unrestricted water).
For both sessions, the investigators will intravenously administer the FDG radioisotope continuously throughout the scan.
Thus, PET will map glucose uptake across the brain, while MRS is simultaneously used to measure production of the neurotransmitters glutamine and GABA.
While having their brains scanned with MR/PET, subjects are initially tested at rest, and then perform a task.
Subjects will drink a ketone sports drink dosed at 395mg/kg.
During the glycolytic (glucose burning) session the same subjects will drink a bolus of glucose, calorie-matched to the ketones.
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Supplement administered mid-scan.
Supplement administered mid-scan.
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この研究は何を測定していますか?
主要な結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
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Diet related changes in brain network stability will be assessed by fMRI BOLD measurements.
時間枠:Within two weeks of enrollment completion
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Changes in BOLD signal measurements will be observed between baseline and during a glycolytic, fasting, or ketotic state.
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Within two weeks of enrollment completion
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Glucose uptake will be measured by PET, with and without ketone supplement
時間枠:Within two weeks of enrollment completion
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Continuous FDG PET infusion is used as a measure of glucose uptake during rest and task, with and without the ketone supplement.
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Within two weeks of enrollment completion
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Neurotransmitter production with and without ketone supplement will be measured by magnetic resonance spectroscopy
時間枠:Within two weeks of enrollment completion
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Changes in neurotransmitter concentration under metabolic demands (i.e., task vs resting-state) with and without ketone supplement will be assessed by magnetic resonance spectroscopy.
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Within two weeks of enrollment completion
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二次結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
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Cognitive performance will be assessed by Mini Mental State Exam (MMSE).
時間枠:Within two weeks of enrollment completion
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All subjects will undergo cognitive testing prior to MRI scanning using the MMSE exam.
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Within two weeks of enrollment completion
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Cognitive performance will be assessed by CNS Vital Signs.
時間枠:Within two weeks of enrollment completion
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All subjects will undergo cognitive testing prior to MRI scanning using the CNS Vital Signs exam.
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Within two weeks of enrollment completion
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Insulin resistance will be assessed by HbA1C.
時間枠:Within two weeks of enrollment completion
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All subjects will be tested for insulin resistance prior to MRI scanning using veinous blood collected and tested for HbA1C.
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Within two weeks of enrollment completion
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Insulin resistance will be assessed by blood insulin level.
時間枠:Within two weeks of enrollment completion
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All subjects will be tested for insulin resistance prior to MRI scanning using veinous blood collected and tested for insulin during a 2-hour Oral Glucose Tolerance Test (OGTT).
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Within two weeks of enrollment completion
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Insulin resistance will be assessed by blood glucose level.
時間枠:Within two weeks of enrollment completion
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All subjects will be tested for insulin resistance prior to MRI scanning using veinous blood collected and tested for glucose during a 2-hour Oral Glucose Tolerance Test (OGTT).
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Within two weeks of enrollment completion
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協力者と研究者
研究記録日
主要日程の研究
研究開始 (実際)
一次修了 (予想される)
研究の完了 (予想される)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (実際)
学習記録の更新
投稿された最後の更新 (実際)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
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