Mutational analysis of the von Willebrand factor gene in type 1 von Willebrand disease using conformation sensitive gel electrophoresis: a comparison of fluorescent and manual techniques
Mohammad Hashemi Soteh, Ian R Peake, Luke Marsden, John Anson, Javier Batlle, Dominique Meyer, Edith Fressinaud, Claudine Mazurier, Jenny Goudemand, Jeroen Eikenboom, Anne Goodeve, MCMDM-1VWD Study Group, Mohammad Hashemi Soteh, Ian R Peake, Luke Marsden, John Anson, Javier Batlle, Dominique Meyer, Edith Fressinaud, Claudine Mazurier, Jenny Goudemand, Jeroen Eikenboom, Anne Goodeve, MCMDM-1VWD Study Group
Abstract
Two versions of conformation sensitive gel electrophoresis, fluorescent (F-CSGE) and manual (M-CSGE) techniques, were compared for mutation analysis of the von Willebrand factor gene. 56 PCRs were used to amplify all 52 exons of the gene in seven type 1 von Willebrand disease cases, plus a healthy control. One hundred and ninety-two samples were analyzed on each F-CSGE gel, compared with 40 on M-CSGE. 125 amplicons revealed bandshifts using F-CSGE, but only 101 by M-CSGE. Five mutations were detected by both techniques. F-CSGE detected 45 different polymorphisms whereas M-CSGE detected only 39. F-CSGE is high-throughput and more sensitive than M-CSGE.
Source: PubMed