Bioluminescence imaging of heme oxygenase-1 upregulation in the Gua Sha procedure

Kenneth K Kwong, Lenuta Kloetzer, Kelvin K Wong, Jia-Qian Ren, Braden Kuo, Yan Jiang, Y Iris Chen, Suk-Tak Chan, Geoffrey S Young, Stephen T C Wong, Kenneth K Kwong, Lenuta Kloetzer, Kelvin K Wong, Jia-Qian Ren, Braden Kuo, Yan Jiang, Y Iris Chen, Suk-Tak Chan, Geoffrey S Young, Stephen T C Wong

Abstract

Gua Sha is a traditional Chinese folk therapy that employs skin scraping to cause subcutaneous microvascular blood extravasation and bruises. The protocol for bioluminescent optical imaging of HO-1-luciferase transgenic mice reported in this manuscript provides a rapid in vivo assay of the upregulation of the heme oxygenase-1 (HO-1) gene expression in response to the Gua Sha procedure. HO-1 has long been known to provide cytoprotection against oxidative stress. The upregulation of HO-1, assessed by the bioluminescence output, is thought to represent an antioxidative response to circulating hemoglobin products released by Gua Sha. Gua Sha was administered by repeated strokes of a smooth spoon edge over lubricated skin on the back or other targeted body part of the transgenic mouse until petechiae (splinter hemorrhages) or ecchymosis (bruises) indicative of extravasation of blood from subcutaneous capillaries was observed. After Gua Sha, bioluminescence imaging sessions were carried out daily for several days to follow the dynamics of HO-1 expression in multiple internal organs.

Figures

https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3149908/bin/jove-30-1385-thumb.jpg

References

    1. Zhang W. Selection of potential therapeutics based on in vivo spatiotemporal transcription patterns of heme oxygenase-1. J Mol Med. 2002;80:655–6564.
    1. Zhang W. Rapid in vivo functional analysis of transgenes in mice using whole body imaging of luciferase expression. Transgenic Res. 2001;10:423–434.
    1. Contag CH, Stevenson DK. In vivo patterns of heme oxygenase-1 transcription. J Perinatol. 2001;21(Suppl 1):119–1124.
    1. Nielsen A, Knoblauch NT, Dobos GJ, Michalsen A, Kaptchuk TJ. The effect of Gua Sha treatment on the microcirculation of surface tissue: a pilot study in healthy subjects. Explore (NY) 2007;3:456–466.
    1. Schwickert ME, Saha FJ, Braun M, Dobos GJ. Gua Sha for migraine in inpatient withdrawal therapy of headache due to medication overuse. Forsch Komplementmed. 2007;14:297–300.
    1. Tsai PS, Lee PH, Wang MY. Demographics, training, and practice patterns of practitioners of folk medicine in Taiwan: a survey of the Taipei metropolitan area. J Altern Complement Med. 2008;14:1243–1248.
    1. Nielsen A. Gua sha research and the language of integrative medicine. J Bodyw Mov Ther. 2009;13:63–72.
    1. Contag CH. Visualizing gene expression in living mammals using a bioluminescent reporter. Photochem Photobiol. 1997;66:523–531.
    1. Cui K, Xu X, Zhao H, Wong ST. A quantitative study of factors affecting in vivo bioluminescence imaging. Luminescence. 2008;23:292–295.
    1. Shibahara S, Yoshida T, Kikuchi G. Mechanism of increase of heme oxygenase activity induced by hemin in cultured pig alveolar macrophages. Arch Biochem Biophys. 1979;197:607–617.
    1. Lee PJ. Hypoxia-inducible factor-1 mediates transcriptional activation of the heme oxygenase-1 gene in response to hypoxia. J Biol Chem. 1997;272:5375–5381.
    1. Maines MD. The heme oxygenase system: a regulator of second messenger gases. Annu Rev Pharmacol Toxicol. 1997;37:517–554.
    1. Wunder C, Potter RF. The heme oxygenase system: its role in liver inflammation. Curr Drug Targets Cardiovasc Haematol Disord. 2003;3:199–208.
    1. Hoekstra KA, Godin DV, Cheng KM. Protective role of heme oxygenase in the blood vessel wall during atherogenesis. Biochem Cell Biol. 2004;82:351–359.
    1. Yang NC, Lu LH, Kao YH, Chau LY. Heme oxygenase-1 attenuates interleukin-1beta-induced nitric oxide synthase expression in vascular smooth muscle cells. J Biomed Sci. 2004;11:799–809.
    1. Morse D, Choi AM. Heme oxygenase-1: from bench to bedside. Am J Respir Crit Care Med. 2005;172:660–670.
    1. Ryter SW. Protective functions of heme oxygenase-1 and carbon monoxide in the respiratory system. Antioxid Redox Signal. 2007;9:2157–2173.
    1. Wilson K, Yu J, Lee A, Wu JC. In vitro and in vivo bioluminescence reporter gene imaging of human embryonic stem cells. J Vis Exp. 2008
    1. Calabrese V. Vitagenes, dietary antioxidants and neuroprotection in neurodegenerative diseases. Front Biosci. 2009;14:376–397.
    1. Contag PR, Olomu IN, Stevenson DK, Contag CH. Bioluminescent indicators in living mammals. Nat Med. 1998;4:245–247.

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