Plant miRNAs found in human circulating system provide evidences of cross kingdom RNAi

Yu-Chen Liu, Wen Liang Chen, Wei-Hsiang Kung, Hsien-Da Huang, Yu-Chen Liu, Wen Liang Chen, Wei-Hsiang Kung, Hsien-Da Huang

Abstract

Background: Emerging evidence indicates that plant miRNAs can present within human circulating system through dietary intake and regulate human gene expression. Hence we deduced that comestible plants miRNAs can be identified in the public available small RNA sequencing data sets.

Results: In this study, we identified abundant plant miRNAs sequences from 410 human plasma small RNA sequencing data sets. One particular plant miRNA miR2910, conserved in fruits and vegetables, was found to present in high relative amount in the plasma samples. This miRNA, with same 6mer and 7mer-A1 target seed sequences as hsa-miR-4259 and hsa-miR-4715-5p, was predicted to target human JAK-STAT signaling pathway gene SPRY4 and transcription regulation genes.

Conclusions: Through analysis of public available plasma small RNA sequencing data, we found the supporting evidence for the plant miRNAs cross kingdom RNAi within human circulating system.

Keywords: Circulating miRNAs; Cross Kingdom RNAi; Plant miRNAs; miR2910.

Figures

Fig. 1
Fig. 1
Predicted target human genes of abundant plant miRNAs. The predicted miR2910, miR2916 and miR2018 targets through miRTar [12] are illustrated in this figure. The pink diamond shape nodes represent plant miRNAs while the round blue nodes represent human genes
Fig. 2
Fig. 2
Summary of the data analysis process. The data analysis pipeline designed to detect the comestible plants miRNAs in human plasma samples is summarized in this figure. The collected human circulating small RNA-seq reads were align to the plant genome. The existence of plant miRNAs was examined through the algorithm of miRDeep2 package [29]

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