Association between BDNF rs6265 and obesity in the Boston Puerto Rican Health Study

Xian-Yong Ma, Wei Qiao Qiu, Caren E Smith, Laurence D Parnell, Zong-Yong Jiang, Jose M Ordovas, Katherine L Tucker, Chao-Qiang Lai, Xian-Yong Ma, Wei Qiao Qiu, Caren E Smith, Laurence D Parnell, Zong-Yong Jiang, Jose M Ordovas, Katherine L Tucker, Chao-Qiang Lai

Abstract

Brain-derived neurotrophic factor (BDNF) has been associated with regulation of body weight and appetite. The goal of this study was to examine the interactions of a functional variant (rs6265) in the BDNF gene with dietary intake for obesity traits in the Boston Puerto Rican Health Study. BDNF rs6265 was genotyped in 1147 Puerto Rican adults and examined for association with obesity-related traits. Men (n = 242) with the GG genotype had higher BMI (P = 0.009), waist circumference (P = 0.002), hip (P = 0.002), and weight (P = 0.03) than GA or AA carriers (n = 94). They had twice the risk of being overweight (BMI ≥ 25) relative to GA or AA carriers (OR = 2.08, CI = 1.02-4.23, and P = 0.043). Interactions between rs6265 and polyunsaturated fatty acids (PUFA) intake were associated with BMI, hip, and weight, and n-3 : n-6 PUFA ratio with waist circumference in men. In contrast, women (n = 595) with the GG genotype had significantly lower BMI (P = 0.009), hip (P = 0.029), and weight (P = 0.027) than GA or AA carriers (n = 216). Women with the GG genotype were 50% less likely to be overweight compared to GA or AA carriers (OR = 0.05, CI = 0.27-0.91, and P = 0.024). In summary, BDNF rs6265 is differentially associated with obesity risk by sex and interacts with PUFA intake influencing obesity traits in Boston Puerto Rican men.

Figures

Figure 1
Figure 1
Association between BDNF genotype and overweight, obesity and abdominal obesity. P value was calculated using logistic regression models, adjusted for age, smoking status, drinking status, sex, education, medication use for depression, physical activity, and population admixture. OR = odds ratios, Lower = Lower 95% limit, Upper = Upper 95% limit, Ab OB = Abdominal obesity. For men, GG versus GA+AA, n = 242 versus 94; for women, GG versus GA + AA, n = 595 versus 216; for men and women combined, GG versus GA+AA, n = 922 versus 337 (n is the sample size).
Figure 2
Figure 2
Interaction between BDNF genotypes and PUFA intake on BMI in the BPRHS. Lozenges represent the major allele GG homozygotes (n = 242 in men; n = 595 in women) and triangles the minor allele A carriers (AA homozygotes and GA heterozygotes combined together, n = 94 in men; women, n = 216 in women). Predicted BMI values for all subjects participants were plotted against their dietary PUFA intake expressed as the percentage of total energy intake. BMI values were calculated based on a regression model containing PUFA intake, BDNF genotypes, and potential confounders including age, smoking status, drinking status, sex, education, medication use for depression, physical activity, total energy, and population admixture.
Figure 3
Figure 3
Interaction between BDNF genotype and n-3 : n-6 PUFA ratio on waist circumference in the BPRHS. Lozenges represent the major allele GG homozygotes (n = 240 in men; n = 585 in women) and triangles the minor allele A carriers (AA homozygotes and GA heterozygotes combined together, n = 92 in men; n = 215 in women). Predicted waist circumference values for all subjects were plotted against their n-3 : n-6 PUFA ratio. Waist values were calculated based on a regression model containing n-3 : n-6 PUFA ratio, BDNF genotypes, and the potential confounders including age, smoking status, drinking status, sex, education, medication use for depression, physical activity, total energy, and population admixture.
Figure 4
Figure 4
Interaction between BDNF genotype and food intake on hip in BPRHS. Lozenges represent the major allele GG homozygotes (n = 242 in men; n = 595 in women) and triangles the minor allele A carriers (AA homozygotes and GA heterozygotes combined together, n = 94 in men; n = 216 in women). Predicted hip values for all subjects were plotted against their food intake expressed as g/d. Hip values were calculated based on the regression model containing PUFA intake, BDNF genotypes, and the potential confounders including age, smoking status, drinking status, sex, education, medication use for depression, physical activity, and population admixture.

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