Change in exhaled nitric oxide during peanut challenge is related to severity of reaction

Elizabeth Percival, Rani Bhatia, Kahn Preece, Mark McEvoy, Adam Collison, Joerg Mattes, Elizabeth Percival, Rani Bhatia, Kahn Preece, Mark McEvoy, Adam Collison, Joerg Mattes

Abstract

Background: Peanut allergy affects 3% of Australian children and has a higher risk of anaphylaxis than most food allergies. Predicting who is likely to develop anaphylaxis is still an inexact science. The fraction of exhaled nitric oxide (FeNO) shows promise as a biomarker involved in peanut allergy, as nitric oxide plays a role in inhibiting mast cell degranulation which is relevant in anaphylaxis, where mast cell degranulation plays a mediator role. The aim of this study was to assess the change in FeNO in children during peanut challenge.

Methods: Thirty-six children aged from 5 to 17 years were recruited for open-labelled peanut challenge. Participants had skin prick test to peanut performed, and serum collected for Ara h2 specific IgE and peanut specific IgE. FeNO was measured by portable device (NIOX VERO) prior to and throughout the peanut challenge.

Results: When grouped according to reaction type at peanut challenge (anaphylaxis, clinical allergy not anaphylaxis and tolerant), there were significant differences in the mean change in FeNO measurement between the anaphylaxis group and the clinical allergy, not anaphylaxis group (p = 0.005), and between the anaphylaxis group and tolerant group (p < 0.0001).

Conclusions: FeNO decreased more significantly in those who subsequently developed anaphylaxis than in those with clinical allergy, not anaphylaxis or negative peanut challenge (tolerance). As a bedside test that can be used in children, it has potential for further research into mechanisms of anaphylaxis in food allergy and potentially assists in predicting an imminent anaphylactic reaction in some patients.Trial registration ClinicalTrials.gov: PEAnut Anaphylaxis Predictors (PEAAP) NCT02424136.

Keywords: Allergy; Anaphylaxis; Ara h2 sIgE; FeNO; Fraction exhaled nitric oxide; Peanut; Peanut sIgE; Skin prick test.

Conflict of interest statement

Competing interestsThe authors declare no competing interests.

© The Author(s) 2020.

Figures

Fig. 1
Fig. 1
Mean change in FeNO (p.p.b) at each increment. Grouped according to reaction type at challenge
Fig. 2
Fig. 2
Mean percentage change in FeNO (p.p.b) at each increment. Grouped according to reaction type at challenge
Fig. 3
Fig. 3
Comparison of combined diagnostic methods by logistic regression analysis, then ROC curve generation for clinical allergy
Fig. 4
Fig. 4
Comparison of combined diagnostic methods by logistic regression analysis, then ROC curve generation for anaphylaxis

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Source: PubMed

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