L-Lysine Ameliorates Diabetic Nephropathy in Rats with Streptozotocin-Induced Diabetes Mellitus

Faezeh Jozi, Nejat Kheiripour, Maryam Akhavan Taheri, Abolfazl Ardjmand, Gholamreza Ghavipanjeh, Zahra Nasehi, Mohammad Esmaeil Shahaboddin, Faezeh Jozi, Nejat Kheiripour, Maryam Akhavan Taheri, Abolfazl Ardjmand, Gholamreza Ghavipanjeh, Zahra Nasehi, Mohammad Esmaeil Shahaboddin

Abstract

Introduction: Diabetic nephropathy is one of the leading causes of end-stage renal disease worldwide. Uncontrolled hyperglycemia and subsequent production of glycation end-products activate the paths which lead to diabetic nephropathy. The aim of this study was to assess the effects of L-lysine on antioxidant capacity, biochemical factors, kidney function, HSP70 level, and the expression of the TGFβ, VEGF, and RAGE genes in rats with streptozocin-induced diabetes mellitus.

Methods: Thirty-two male Wistar rats were randomly allocated to four eight-rat groups, namely, a healthy group, a diabetic group treated with vehicle (DM + vehicle), a diabetic group treated with L-lysine (DM + Lys), and a healthy group treated with L-lysine (healthy + Lys). Rats in the DM + Lys and the healthy + Lys groups were treated with L-lysine 0.15%. The levels of fasting blood glucose, insulin, HbA1C, advanced glycation end-products (AGEs), lipid profile, serum creatinine, blood urea nitrogen, glomerular filtration rate, urine microalbumin, oxidative stress parameters, kidney histology and morphology, and TGFβ, VEGF, and RAGE gene expressions were assessed. Findings. An eight-week treatment with L-lysine significantly reduced the levels of fasting blood glucose, AGEs, kidney function parameters, oxidative stress parameters, lipid profile, and the TGFβ, VEGF, and RAGE gene expression and significantly increased the levels of serum insulin and tissue HSP70.

Conclusion: Treatment with L-lysine seems to slow down the progression of diabetic nephropathy.

Conflict of interest statement

The authors declare no conflict of interests.

Copyright © 2022 Faezeh Jozi et al.

Figures

Figure 1
Figure 1
Level of fasting blood glucose in different groups (A#: significant difference between the healthy group and other groups; B#: significant difference between the DM + Vehicle group and other groups).
Figure 2
Figure 2
The expression of the RAGE (a), TGF (b), and VEGF (c) genes in kidney tissue (#: significant difference between the DM + Vehicle group and other groups).
Figure 3
Figure 3
Histological morphology of kidney tissue in the healthy group (a), healthy + Lys group (b), DM + vehicle group (c and d), and DM + Lys group (e and f) (magnification: ×400).

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