Licochalcone-A induces intrinsic and extrinsic apoptosis via ERK1/2 and p38 phosphorylation-mediated TRAIL expression in head and neck squamous carcinoma FaDu cells
Mi-Ra Park, Su-Gwan Kim, In-A Cho, Dahye Oh, Kyeong-Rok Kang, Sook-Young Lee, Sung-Min Moon, Seung Sik Cho, Goo Yoon, Chun Sung Kim, Ji-Su Oh, Jae-Seek You, Do Kyung Kim, Yo-Seob Seo, Hee-Jeong Im, Jae-Sung Kim, Mi-Ra Park, Su-Gwan Kim, In-A Cho, Dahye Oh, Kyeong-Rok Kang, Sook-Young Lee, Sung-Min Moon, Seung Sik Cho, Goo Yoon, Chun Sung Kim, Ji-Su Oh, Jae-Seek You, Do Kyung Kim, Yo-Seob Seo, Hee-Jeong Im, Jae-Sung Kim
Abstract
We investigated Licochalcone-A (Lico-A)-induced apoptosis and the pathway underlying its activity in a pharyngeal squamous carcinoma FaDu cell line. Lico-A purified from root of Glycyrrhiza inflata had cytotoxic effects, significantly increasing cell death in FaDu cells. Using a cell viability assay, we determined that the IC50 value of Lico-A in FaDu cells was approximately 100 µM. Chromatin condensation was observed in FaDu cells treated with Lico-A for 24 h. Consistent with this finding, the number of apoptotic cells increased in a time-dependent manner when FaDu cells were treated with Lico-A. TRAIL was significantly up-regulated in Lico-A-treated FaDu cells in a dose-dependent manner. Apoptotic factors such as caspases and PARP were subsequently activated in a caspase-dependent manner. In addition, levels of pro-apoptotic factors increased significantly in response to Lico-A treatment, while levels of anti-apoptotic factors decreased. Lico-A-induced TRAIL expression was mediated in part by a MAPK signaling pathway involving ERK1/2 and p38. In xenograft mouse model, Lico-A treatment effectively suppressed the growth of FaDu cell xenografts by activating caspase-3, without affecting the body weight of mice. Taken together, these data suggest that Lico-A has potential chemopreventive effects and should therefore be developed as a chemotherapeutic agent for pharyngeal squamous carcinoma.
Keywords: Apoptosis; Chemoprevention; FaDu cells; Licochalcone-A; Pharyngeal squamous carcinoma.
Conflict of interest statement
Conflict of Interest
The authors declare that there are no conflicts of interest.
Copyright © 2014 Elsevier Ltd. All rights reserved.
Figures
![Fig. 1](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/4522946/bin/nihms711287f1.jpg)
Fig. 2
Lico-A-induced FaDu cell death is…
Fig. 2
Lico-A-induced FaDu cell death is mediated by both intrinsic and extrinsic apoptotic pathways.…
Fig. 3
Lico-A-induced apoptosis in FaDu cells…
Fig. 3
Lico-A-induced apoptosis in FaDu cells is dependent on caspase activation. (A) Lico-A induces…
Fig. 4
Lico-A-induced FaDu cell apoptosis is…
Fig. 4
Lico-A-induced FaDu cell apoptosis is regulated by TRAIL expression induced by ERK1/2 and…
Fig. 5
Lico-A inhibits the volume of…
Fig. 5
Lico-A inhibits the volume of FaDu tumors in a xenografted animal model. (A)…
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Apoptotic signaling pathway induced by…
Fig. 6
Apoptotic signaling pathway induced by Licochalcone-A in head and neck squamous carcinoma FaDu…
![Fig. 2](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/4522946/bin/nihms711287f2.jpg)
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Source: PubMed