Flavan-3-ol compounds from wine wastes with in vitro and in vivo antioxidant activity

Gustavo Scola, Danusa Conte, Patrícia Wilmsen Dalla-Santa Spada, Caroline Dani, Regina Vanderlinde, Claudia Funchal, Mirian Salvador, Gustavo Scola, Danusa Conte, Patrícia Wilmsen Dalla-Santa Spada, Caroline Dani, Regina Vanderlinde, Claudia Funchal, Mirian Salvador

Abstract

It has been suggested that the dietary intake of antioxidant supplements could be a useful strategy to reduce the incidence of diseases associated with oxidative stress. The aim of present work is to study the possibility to obtain compounds with antioxidant activity from wine wastes using water as solvent. Results have shown that it is possible to obtain flavan-3-ol compounds from wine wastes both from V. vinifera (cv. Cabernet Sauvignon and Merlot) and V. labrusca (cv. Bordo and Isabella) species. The main phenolic compounds found in the extracts were catechin and epicatechin, followed by procyanidin B3, procyanidin B1, procyanidin B2, gallic acid, epigallocatechin, and procyanidin B4. All flavan-3-ol extracts showed significant in vitro and in vivo activities. It was found that the extracts were able to prevent lipid and protein oxidative damage in the cerebral cortex, cerebellum and hippocampus tissues of rats. Although further studies are necessary, these flavan-3-ol extracts show potential to be used to reduce the incidence of degenerative diseases associated with oxidative stress.

Keywords: V. labrusca; V. vinifera; antioxidant; aqueous wine waste extracts; phenolic content.

Figures

Figure 1
Figure 1
In vitro antioxidant activity of wine waste extracts. IC50 is the amount (%) of extracts needed to scavenge 50% of DPPH˙. Catechin was used as control. The results represent mean ± S.D. of three independent experiments. Different letters are statistically different by analysis of variance (ANOVA) and Tukey’s post-hoc test (p ≤ 0.05).
Figure 2
Figure 2
Survival of S. cerevisiae cells treated with wine waste extracts plus hydrogen peroxide. Data are mean ± S.D. Different letters indicate significant differences using analysis of variance (ANOVA) and Tukey’s post-hoc test (p ≤ 0.05).

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