Effect of Topical Platelet-Rich Plasma on Burn Healing After Partial-Thickness Burn Injury

Umit Ozcelik, Yahya Ekici, Huseyin Yuce Bircan, Cem Aydogan, Suna Turkoglu, Ozlem Ozen, Gokhan Moray, Mehmet Haberal, Umit Ozcelik, Yahya Ekici, Huseyin Yuce Bircan, Cem Aydogan, Suna Turkoglu, Ozlem Ozen, Gokhan Moray, Mehmet Haberal

Abstract

BACKGROUND To investigate the effects of platelet-rich plasma on tissue maturation and burn healing in an experimental partial-thickness burn injury model. MATERIAL AND METHODS Thirty Wistar albino rats were divided into 3 groups of 10 rats each. Group 1 (platelet-rich plasma group) was exposed to burn injury and topical platelet-rich plasma was applied. Group 2 (control group) was exposed to burn injury only. Group 3 (blood donor group) was used as blood donors for platelet-rich plasma. The rats were killed on the seventh day after burn injury. Tissue hydroxyproline levels were measured and histopathologic changes were examined. RESULTS Hydroxyproline levels were significantly higher in the platelet-rich plasma group than in the control group (P=.03). Histopathologically, there was significantly less inflammatory cell infiltration (P=.005) and there were no statistically significant differences between groups in fibroblast development, collagen production, vessel proliferations, or epithelization. CONCLUSIONS Platelet-rich plasma seems to partially improve burn healing in this experimental burn injury model. As an initial conclusion, it appears that platelet-rich plasma can be used in humans, although further studies should be performed with this type of treatment.

Figures

Figure 1
Figure 1
Tissue hydroxyproline levels of the groups.
Figure 2
Figure 2
Distribution of polymorphonuclear leucocytes infiltration, fibroblasts, vessel proliferation, and collagen deposit in both groups.
Figure 3
Figure 3
Moderate inflammatory cell infiltration in the control group (H-E ×40, group 2).
Figure 4
Figure 4
Mild PMNL infiltration in the study group (H-E ×40, group 1).
Figure 5
Figure 5
Focal fibroblast infiltration in the study group (H-E ×40, group 1).
Figure 6
Figure 6
Mild vessel proliferation in the study group (Masson trichrome ×40, group 1).
Figure 7
Figure 7
Mild collagen deposition in the study group (Masson trichrome ×40, group 1).

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