Assignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5

V L Funanage, T T Myoda, P A Moses, H R Cowell, V L Funanage, T T Myoda, P A Moses, H R Cowell

Abstract

Cells from a dihydrofolate reductase-deficient Chinese hamster ovary cell line were hybridized to human fetal skin fibroblast cells. Nineteen dihydrofolate reductase-positive hybrid clones were isolated and characterized. Cytogenetic and biochemical analyses of these clones have shown that the human dihydrofolate reductase (DHFR) gene is located on chromosome 5. Three of these hybrid cell lines contained different terminal deletions of chromosome 5. An analysis of the breakpoints of these deletions has demonstrated that the DHFR gene resides in the q11----q22 region.

References

    1. J Biol Chem. 1966 Mar 25;241(6):1319-28
    1. Cancer Res. 1966 Aug;26(8):1653-60
    1. Arch Biochem Biophys. 1971 Feb;142(2):417-25
    1. Nat New Biol. 1972 Jul 26;238(82):122-4
    1. Chromosoma. 1973;41(2):129-44
    1. Humangenetik. 1975 Sep 23;29(3):229-31
    1. Anal Biochem. 1976 May 7;72:248-54
    1. Cytogenet Cell Genet. 1976;16(1-5):83-91
    1. Exp Cell Res. 1977 Feb;104(2):255-62
    1. Somatic Cell Genet. 1976 May;2(3):271-80
    1. Cytogenet Cell Genet. 1977;19(4):236-9
    1. Somatic Cell Genet. 1977 Jan;3(1):27-45
    1. J Biol Chem. 1978 Mar 10;253(5):1357-70
    1. Cytogenet Cell Genet. 1978;21(1-2):105-10
    1. Nature. 1978 Oct 19;275(5681):617-24
    1. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5553-6
    1. Methods Enzymol. 1979;58:322-44
    1. J Cell Biol. 1979 Nov;83(2 Pt 1):394-402
    1. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5669-73
    1. Cell. 1980 Feb;19(2):355-64
    1. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4216-20
    1. Nucleic Acids Res. 1981 Mar 25;9(6):1311-22
    1. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6043-7
    1. Anal Biochem. 1981 Sep 15;116(2):298-302
    1. J Mol Biol. 1981 Dec 5;153(2):203-18
    1. J Cell Biol. 1982 Aug;94(2):418-24
    1. Mol Cell Biol. 1982 Mar;2(3):275-85
    1. Mol Cell Biol. 1982 May;2(5):498-507
    1. J Mol Biol. 1982 Apr 15;156(3):583-607
    1. Mol Pharmacol. 1982 Jan;21(1):231-8
    1. Mol Cell Biol. 1982 Oct;2(10):1220-8
    1. Proc Natl Acad Sci U S A. 1983 Feb;80(3):807-9
    1. Nucleic Acids Res. 1983 Apr 11;11(7):1997-2012
    1. Am J Hum Genet. 1983 May;35(3):385-92
    1. Cell. 1983 Jun;33(2):405-12
    1. Mol Cell Biol. 1983 Jul;3(7):1266-73
    1. Mol Cell Biol. 1983 Jul;3(7):1274-82
    1. Biochemistry. 1983 Dec 6;22(25):5774-81
    1. Mol Cell Biol. 1984 Jan;4(1):69-76
    1. J Biol Chem. 1984 Mar 25;259(6):3933-43
    1. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1484-8
    1. Mol Cell Biol. 1981 Apr;1(4):330-5
    1. Biochim Biophys Acta. 1962 Apr 9;58:377-80
    1. Somatic Cell Genet. 1976 Mar;2(2):183-8

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