Urinary expression of novel tissue markers of kidney injury after ureteroscopy, shockwave lithotripsy, and in normal healthy controls

Abstract

Background and purpose: Shockwave lithotripsy (SWL) and ureteroscopy (URS) are minimally invasive treatment alternatives for kidney stones. Although less invasive, SWL subjects the renal parenchyma to a high level of energy and the potential to cause renal injury. The ability to detect renal injury post-SWL in a reliable and noninvasive way would be clinically beneficial. Kidney injury molecule 1 (KIM-1) and N-acetyl-β-D-glucosaminidase (NAG) are two proteins secreted by the kidney into the urine and have been found to be sensitive markers of acute kidney injury in transplant patients. The aim of this work was to measure urinary levels of KIM-1 and NAG in patients with kidney stone who were treated by SWL or URS and in nonstone volunteers.

Patients and methods: Patients with kidney stones who were treated by SWL (n = 50) or URS (n = 10) were recruited. Voided urine samples were collected before and 2 to 3 hours after URS and SWL. In addition, further urinary specimens were collected 2 days and 2 weeks post-SWL treatment. Voided urine samples from healthy volunteers were also collected.

Results: Mean KIM-1 values were increased in patients with kidney stones when compared with volunteers. KIM-1 and NAG levels significantly increased post-SWL and returned to baseline within 2 weeks post-SWL. Poor kidney function was significantly associated with increased biomarker activity both in baseline and post-SWL measurements. There was no significant change in urinary KIM-1 and NAG concentrations before and after URS.

Conclusions: Kim-1 and NAG levels significantly increased post-SWL treatment suggesting a potential role for these urinary markers in identifying patients at higher risk of tissue injury.

Figures

FIG. 1.

Mean urinary biomarker concentrations in relation to shockwave lithotripsy (SWL) treatment. Treatment arm (A) and (C). Follow-up arm (B) and (D). Pg=pictogram; mU=milliunits; uCr=urinary creatinine; KIM-1=kidney injury molecule 1; NAG=N-acetyl-β-D-glucosaminidase.

FIG. 2.

Mean urinary biomarker concentrations among stone patients and volunteer group. Pg=pictogram; mU=milliunits; uCr=urinary creatinine.

FIG. 3.

Mean urinary biomarkers in relation to chronic kidney injury (CKI) stage. Treatment arm (A) and (C). Follow-up arm (B) and (D). Pg=pictogram; mU=milliunits; uCr=urinary creatinine; KIM-1=kidney injury molecule 1; NAG=N-acetyl-β-D-glucosaminidase.