Lateral and axial measurement differences between spectral-domain optical coherence tomography systems

Abstract

We assessed the reproducibility of lateral and axial measurements performed with spectral-domain optical coherence tomography (SDOCT) instruments from a single manufacturer and across several manufacturers. One human retina phantom was imaged on two instruments each from four SDOCT platforms: Zeiss Cirrus, Heidelberg Spectralis, Bioptigen SDOIS, and hand-held Bioptigen Envisu. Built-in software calipers were used to perform manual measurements of a fixed lateral width (LW), central foveal thickness (CFT), and parafoveal thickness (PFT) 1 mm from foveal center. Inter- and intraplatform reproducibilities were assessed with analysis of variance and Tukey-Kramer tests. The range of measurements between platforms was 5171 to 5290 μm for mean LW (p<0.001), 162 to 196 μm for mean CFT (p<0.001), and 267 to 316 μm for mean PFT (p<0.001). All SDOCT platforms had significant differences between each other for all measurements, except LW between Bioptigen SDOIS and Envisu (p=0.27). Intraplatform differences were significantly smaller than interplatform differences for LW (p=0.020), CFT (p=0.045), and PFT (p=0.004). Conversion factors were generated for lateral and axial scaling between SDOCT platforms. Lateral and axial manual measurements have greater variance across different SDOCT platforms than between instruments from the same platform. Conversion factors for measurements from different platforms can produce normalized values for patient care and clinical studies.

Figures

Fig. 1

Model eye measurements were obtained for the fixed lateral width, center foveal thickness, and parafoveal thickness 1 mm to the left and right of center. The B-scan with the widest width and minimum central thickness of the circular tissue phantom was selected for measurements. Lateral width was defined as the horizontal distance at the base. Axial thickness was defined as the vertical distance from the inner border of the hyperreflective inner retinal surface to the inner border of the hyperreflective base substrate beneath the tissue phantom. Values shown here are mean values obtained with each spectral-domain optical coherence tomography platform.