The serum angiotensin converting enzyme and lysozyme levels in patients with ocular involvement of autoimmune and infectious diseases

Ozlem Sahin, Alireza Ziaei, Eda Karaismailoğlu, Nusret Taheri, Ozlem Sahin, Alireza Ziaei, Eda Karaismailoğlu, Nusret Taheri

Abstract

Background: Increased serum levels of angiotensin converting enzyme and lysozyme are considered as inflammatory markers for diagnosis of sarcoidosis which is an autoimmune inflammatory disease. The purpose of this study is to evaluate the significance of differences in serum angiotensin converting enzyme and lysozyme levels of patients with ocular involvement of other autoimmune inflammatory and infectious diseases.

Methods: This is a prospective study involving patients with ankylosing spondylitis, behcet's disease, presumed sarcoidosis, presumed latent tuberculosis, presumed latent syphilis, and control group. The serum levels of angiotensin converting enzyme and lysozyme were analyzed by enzyme-linked immunosorbent assay. Bonnferoni analysis was used to assess pairwise comparisons between the groups.

Results: There was a significant increase in serum angiotensin converting enzyme level in patients with presumed sarcoidosis compared to ankylosing spondylitis (p = 0.0001), behcet's disease (p = 0.0001), presumed latent tuberculosis (p = 0.0001), presumed latent syphilis (p = 0.0001), and control group (p = 0.0001). The increase in serum lysozyme level was significant for patients with presumed sarcoidosis with respect to ankylosing spondylitis (p = 0.0001), behcet's disease, (p = 0.0001) presumed latent tuberculosis (p = 0.001), presumed latent syphilis (p = 0.033), and control group (p = 0.0001).

Conclusion: Elevated serum angiotensin converting enzyme levels are significant for patients with presumed sarcoidosis compared to ocular involvement of other autoimmune diseases such as behcet's disease and ankylosing spondylitis, and ocular involvement of infectious diseases such as presumed latent tuberculosis and presumed latent syphilis. However, elevated serum lysozyme level might be also detected in ocular involvement of infectious diseases such as presumed latent tuberculosis and presumed latent syphilis.

Trial registration number: NCT02627209. Date of registration: 12/09/2015.

Figures

Fig. 1
Fig. 1
The estimated marginal means of serum angiotensin converting enzyme levels of patients with ocular involvement of ankylosing spondylitis, behcet’s disease, presumed sarcoidosis, presumed latent tuberculosis, presumed latent syphilis and control group
Fig. 2
Fig. 2
The estimated marginal means of serum lysozyme levels of patients with ocular involvement of ankylosing spondylitis, behcet’s disease, presumed sarcoidosis, presumed latent tuberculosis, presumed latent syphilis and control group

References

    1. Chappell MC, Marshall AC, Alzayadneh EM, Shaltout HA, Diz DI. Update on the Angiotensin Converting Enzyme 2-Angiotensin (1–7)-Mas Receptor Axis: Fetal programming, sex differences, and intracellular pathways. Front Endocrinol (Lausanne) 2014;4:201.
    1. Miyazaki A, Kitaichi N, Ohgami K, Iwata D, Jin XH, Iwabuchi K, et al. Anti-inflammatory effect of angiotensin type 1 receptor antagonist on endotoxin-induced uveitis in rats. Graefes Arch Clin Exp Ophthalmol. 2008;246:747–57. doi: 10.1007/s00417-007-0730-2.
    1. Kurihara T, Ozawa Y, Ishida S, Okano H, Tsubota K. Renin-Angiotensin system hyperactivation can induce inflammation and retinal neural dysfunction. Int J Inflam. 2012
    1. Bonfiolli AA, Orefice F. Sarcoidosis. Semin Ophthalmol. 2005;20:177–82. doi: 10.1080/08820530500231938.
    1. Mulak M, Misiuk-Hojlo M, Slowik M. The behavior of angiotensin converting enzyme in patients with uveitis. Klin Oczna. 1999;101:5–8.
    1. Nash JA, Ballard TN, Weaver TE, Akinbi HT. The peptidoglycan degrading property of lysozyme is not required for bacteriocidal activity in vivo. J Immunol. 2006;177:519–26. doi: 10.4049/jimmunol.177.1.519.
    1. Majcherczyk PA, Langen H, Heumann M, Fountoulakis M, Glauser P, Moreillion P. Digestion of Streptococcus pneumonia cell walls with its major peptidoglycan hydrolase releases branched stem peptides carrying proinflammatory activity. J Biol Chem. 1999;274:12537–43. doi: 10.1074/jbc.274.18.12537.
    1. Sen DK, Sarin GS. Immunoassay of serum muramidase (lysozyme) in ocular diseases. Indian J Ophthalmol. 1987;35:103–7.
    1. Herbort CP, Rao NA, Mochizuki M, members of Scientific Committee of First International Workshop on ocular sarcoidosis International criteria for the diagnosis of ocular sarcoidosis: results of the first ınternational Workshop on ocular sarcoidosis (IWOS) Ocul Immunol Inflamm. 2009;17:160–9. doi: 10.1080/09273940902818861.
    1. [No authors listed] Evaluation of the diagnostic (‘classification’) criteria in Behcet’s disease-towards internationally agreed criteria. The International Study Group for Behcet’s disease. Br J Rheumatol 1992;31:299–308.
    1. Morshed MG. Current trend on syphilis diagnosis: issues and challenges. Adv Exp Med Biol. 2014;808:51–64. doi: 10.1007/978-81-322-1774-9_5.
    1. Beneteau B, Baudin B, Morgant G, Giboudeau J, Baumann FC. Automated kinetic assay of angiotensin-converting enzyme in serum. Clin Chem. 1986;32:884–6.
    1. Mancini G, Carbonara AO, Heremans JF. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965;2:235–54. doi: 10.1016/0019-2791(65)90004-2.
    1. Takeuchi M, Oh-I K, Suzuki J, Hattori D, Takeuchi A, Okunuki Y, et al. Elevated serum levels of CXCL9/monokine induced by interferon- Gamma and CXCL10/interferon gamma-inducible protein-10 in ocular sarcoidosis. Invest Ophthalmol Vis Sci. 2006;47:1063–8. doi: 10.1167/iovs.05-0966.
    1. Trinchieri G. Interleukin-12: a cytokine produced by antigen-presenting cells with immunoregulatory functions in the generation of T-helper cells type 1 and cytotoxic lymphocytes. Blood. 1994;84:4008–27.
    1. Murdoch C, Finn A. Chemokine receptors and their role in inflammation and infectious diseases. Blood. 2000;95:3032–43.
    1. Gerard C, Rollins BJ. Chemokines and disease. Nat Immunol. 2001;2:108–15. doi: 10.1038/84209.
    1. Ichimura S, et al. Increased circulating interleukin-12 (IL-12) p40 in pulmonary sarcoidosis. Clin Exp Immunol. 2003;132:152–7. doi: 10.1046/j.1365-2249.2003.02105.x.
    1. Stavrou P, Linton S, Young DW, Murray PI. Clinical diagnosis of ocular sarcoidosis. Eye (Lond) 1997;11:365–70. doi: 10.1038/eye.1997.77.
    1. Weinreb RN, O’Donnell JJ, Sandman R, Char DH, Kimura SJ. Angiotensin-converting enzyme in sarcoid uveitis. Invest Ophthalmol Vis Sci. 1979;18:1285–7.
    1. Power WJ, Neves RA, Rodriquez A, Pedrosa-Seres M, Foster CS. The value of combined serum angiotensin-converting enzyme and gallium scan in diagnosing ocular sarcoidosis. Ophthalmology. 1995;102:2007–11. doi: 10.1016/S0161-6420(95)30763-4.
    1. Deverriere G, Flamans-Klein A, Firmin D, Azouzi O, Courville P, Le Roux P. Early onset pediatric sarcoidosis, diagnostic problems. Arch Pediatr. 2012;19:707–10. doi: 10.1016/j.arcped.2012.04.024.
    1. Hunninghake GW, Costabel U, Ando M, Baughman R, Cordier JF, du Bois R, et al. ATS/ERS/WASOG statement on sarcoidosis. Sarcoidosis Vasc Diffuse Lung Dis. 1999;16:149–73.
    1. Adamovich VN, Borisov SE, Zubkov AA, Danilov SM, Sakharov II, Atochina EN. Serum angiotensin converting enzyme in the diagnosis of sarcoidosis and other lung diseases. Probl Tuberk. 1991;10:18–22.
    1. Fukami R, Ohba S, Ishida K, Nakamura S, Konno M, Ohno S. Serum adenosine deaminase and angiotensin converting enzyme activity in patients with endogenous uveitis. Nihon Ganka Gakkai Zasshi. 1994;98:287–92.
    1. Lowe JR, Dixon JS, Guthrie JA, McWhinney P. Serum and synovial fluid levels of angiotensin converting enzyme in polyarthritis. Ann Rheum Dis. 1986;45:921–4. doi: 10.1136/ard.45.11.921.
    1. Tomita H, Sato S, Matsuda R, Suquira R, Kawaquichi H, Niimi T, et al. Serum lysozyme levels and clinical features of sarcoidosis. Lung. 1999;177:161–7. doi: 10.1007/PL00007637.
    1. Birnbaum AD, Oh FS, Chakrabarti A, Tessler HH, Goldstein DA. Clinical features and diagnostic evaluation of biopsy-proven ocular sarcoidosis. Arch Ophthalmol. 2011;129:409–13. doi: 10.1001/archophthalmol.2011.52.
    1. Baarsma GS, La Hey E, Glasius E, de Vries J, Kijlstra A. The predictive value of serum angiotensin converting enzyme and lysozyme levels in the diagnosis of ocular sarcoidosis. Am J Ophthalmol. 1987;104:211–7. doi: 10.1016/0002-9394(87)90406-5.

Source: PubMed

Спонсоры и соавторы

Заболевания

Медикаментозные вмешательства

Подписаться