Measurement of Reactive Oxygen Species, Reactive Nitrogen Species, and Redox-Dependent Signaling in the Cardiovascular System: A Scientific Statement From the American Heart Association
Kathy K Griendling, Rhian M Touyz, Jay L Zweier, Sergey Dikalov, William Chilian, Yeong-Renn Chen, David G Harrison, Aruni Bhatnagar, American Heart Association Council on Basic Cardiovascular Sciences, Kathy K Griendling, Rhian M Touyz, Jay L Zweier, Sergey Dikalov, William Chilian, Yeong-Renn Chen, David G Harrison, Aruni Bhatnagar, American Heart Association Council on Basic Cardiovascular Sciences
Abstract
Reactive oxygen species and reactive nitrogen species are biological molecules that play important roles in cardiovascular physiology and contribute to disease initiation, progression, and severity. Because of their ephemeral nature and rapid reactivity, these species are difficult to measure directly with high accuracy and precision. In this statement, we review current methods for measuring these species and the secondary products they generate and suggest approaches for measuring redox status, oxidative stress, and the production of individual reactive oxygen and nitrogen species. We discuss the strengths and limitations of different methods and the relative specificity and suitability of these methods for measuring the concentrations of reactive oxygen and reactive nitrogen species in cells, tissues, and biological fluids. We provide specific guidelines, through expert opinion, for choosing reliable and reproducible assays for different experimental and clinical situations. These guidelines are intended to help investigators and clinical researchers avoid experimental error and ensure high-quality measurements of these important biological species.
Keywords: AHA Scientific Statements; free radicals; oxidants; peroxides; reactive nitrogen species; reactive oxygen species.
Conflict of interest statement
The American Heart Association makes every effort to avoid any actual or potential conflicts of interest that may arise as a result of an outside relationship or a personal, professional, or business interest of a member of the writing panel. Specifically, all members of the writing group are required to complete and submit a Disclosure Questionnaire showing all such relationships that might be perceived as real or potential conflicts of interest.
© 2016 American Heart Association, Inc.
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Source: PubMed