Decreased pregnancy and live birth rates after vitrification of in vitro matured oocytes

Yoni Cohen, Alexandra St-Onge-St-Hilaire, Samer Tannus, Grace Younes, Michael H Dahan, William Buckett, Weon-Young Son, Yoni Cohen, Alexandra St-Onge-St-Hilaire, Samer Tannus, Grace Younes, Michael H Dahan, William Buckett, Weon-Young Son

Abstract

Purpose: To assess effects on fertilization rate, embryo quality, pregnancy, and live birth rates of vitrification and warming of oocytes that matured in vitro (vIVM) compared to fresh in vitro maturation (fIVM) cycles.

Methods: A retrospective cohort study conducted at a university hospital-affiliated IVF unit. Fifty-six cycles of vIVM cycles and 263 fIVM in women diagnosed with polycystic ovarian syndrome (PCOS) ovaries were included in the analysis. The study group included PCOS patients who failed ovulation induction with intrauterine insemination and were offered IVM cycle followed by oocyte vitrification and warming. The embryological aspects and clinical outcomes were compared to those of controls undergoing fresh IVM cycles during the same period. The main outcome measure was live birth rate.

Results: One thousand seventy oocytes were collected from 56 patients and underwent vitrification and warming. In the control group, 4781 oocytes were collected from 219 patients who had undergone a fresh IVM cycle. Oocyte maturation rates were similar between the groups (mean ± SD: 0.7 ± 0.2 vs. 0.6 ± 0.2, for vIVM and fIVM, respectively). Survival rate after warming was 59.8%. Fertilization and embryo cleavage rates per oocyte were significantly lower in the vIVM group. Clinical pregnancy (10.7 vs. 36.1%) and live birth rates (8.9 vs. 25.9%) per cycle were significantly lower in the vIVM group than those in the fIVM group (P = 0.005 and P < 0.001, respectively). Five healthy babies were born in the vIVM group.

Conclusions: The reproductive potential of vitrified IVM oocytes is impaired. This injury likely occurs through vitrification and warming.

Keywords: Cryopreservation; In vitro maturation; Live birth rate; Oocyte; PCOS; Vitrification.

Conflict of interest statement

Conflict of interest

The authors declare that they have no conflict of interest.

References

    1. Liu J, Lu G, Qian Y, Mao Y, Ding W. Pregnancies and births achieved from in vitro matured oocytes retrieved from poor responders undergoing stimulation in in vitro fertilization cycles. Fertil Steril. 2003;80:447–449. doi: 10.1016/S0015-0282(03)00665-4.
    1. De Vos M, Smitz J, Woodruff TK. Fertility preservation in women with cancer. Lancet. 2014;384:1302–1310. doi: 10.1016/S0140-6736(14)60834-5.
    1. Creux H, Monnier P, Son WY, Tulandi T, Buckett W. Immature oocyte retrieval and in vitro oocyte maturation at different phases of the menstrual cycle in women with cancer who require urgent gonadotoxic treatment. Fertil Steril. 2017;107:198–204. doi: 10.1016/j.fertnstert.2016.09.041.
    1. Abir R, Ben-Aharon I, Garor R, Yaniv I, Ash S, Stemmer SM, Ben-Haroush A, Freud E, Kravarusic D, Sapir O, Fisch B. Cryopreservation of in vitro matured oocytes in addition to ovarian tissue freezing for fertility preservation in paediatric female cancer patients before and after cancer therapy. Hum Reprod. 2016;31:750–762. doi: 10.1093/humrep/dew007.
    1. Cobo A, García-Velasco JA, Coello A, Domingo J, Pellicer A, Remohí J. Oocyte vitrification as an efficient option for elective fertility preservation. Fertil Steril. 2016;105:755–764e8. doi: 10.1016/j.fertnstert.2015.11.027.
    1. Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efficient vitrification method for cryopreservation of human oocytes. Reprod BioMed Online. 2005;11:300–308. doi: 10.1016/S1472-6483(10)60837-1.
    1. Shalom-Paz E, Almog B, Shehata F, Huang J, Holzer H, Chian RC, Son WY, Tan SL. Fertility preservation for breast-cancer patients using IVM followed by oocyte or embryo vitrification. Reprod BioMed Online. 2010;21:566–571. doi: 10.1016/j.rbmo.2010.05.003.
    1. Ali A, Benkhalifa M, Miron P. In-vitro maturation of oocytes: biological aspects. Reprod BioMed Online. 2006;13:437–446. doi: 10.1016/S1472-6483(10)61450-2.
    1. Sanfins A, Plancha CE, Overstrom EW, Albertini DF. Meiotic spindle morphogenesis in in vivo and in vitro matured mouse oocytes: insights into the relationship between nuclear and cytoplasmic quality. Hum Reprod. 2004;19:2889–2899. doi: 10.1093/humrep/deh528.
    1. Walls ML, Ryan JP, Keelan JA, Hart R. In vitro maturation is associated with increased early embryo arrest without impairing morphokinetic development of useable embryos progressing to blastocysts. Hum Reprod. 2015;30:1842–1849. doi: 10.1093/humrep/dev125.
    1. Walls ML, Hart R, Keelan JA, Ryan JP. Structural and morphologic differences in human oocytes after in vitro maturation compared with standard in vitro fertilization. Fertil. Steril. 2016;106:1392–1398.e5. doi: 10.1016/j.fertnstert.2016.08.014.
    1. Brambillasca F, Guglielmo MC, Coticchio G, Mignini Renzini M, Dal Canto M, Fadini R. The current challenges to efficient immature oocyte cryopreservation. J Assist Reprod Genet. 2013;30:1531–1539. doi: 10.1007/s10815-013-0112-0.
    1. Lei T, Guo N, Liu J-Q, Tan M-H, Li Y-F. Vitrification of in vitro matured oocytes: effects on meiotic spindle configuration and mitochondrial function. Int J Clin Exp Pathol. 2014;7:1159–1165.
    1. Chian RC, Huang JYJ, Gilbert L, Son WY, Holzer H, Cui SJ, Buckett WM, Tulandi T, Tan SL. Obstetric outcomes following vitrification of in vitro and in vivo matured oocytes. Fertil Steril. 2009;91:2391–2398. doi: 10.1016/j.fertnstert.2008.04.014.
    1. Rotterdam ESHRE/ASRM-Sponsored PCOS consensus workshop group Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS) Hum Reprod. 2004;19:41–47. doi: 10.1093/humrep/deh098.
    1. Son WY, Chung JT, Demirtas E, Holzer H, Sylvestre C, Buckett W, Chian RC, Tan SL. Comparison of in-vitro maturation cycles with and without in-vivo matured oocytes retrieved. Reprod BioMed Online. 2008;17:59–67. doi: 10.1016/S1472-6483(10)60294-5.
    1. Son WY, Tan SL. Laboratory and embryological aspects of hCG-primed in vitro maturation cycles for patients with polycystic ovaries. Hum Reprod Update. 2010;16:675–689. doi: 10.1093/humupd/dmq014.
    1. Shalom-Paz E, Holzer H, Young Son W, Levin I, Tan SL, Almog B. PCOS patients can benefit from in vitro maturation (IVM) of oocytes. Eur J Obstet Gynecol Reprod Biol. 2012;165:53–56. doi: 10.1016/j.ejogrb.2012.07.001.
    1. Cao Y, Xing Q, Zhang Z-G, Wei Z-L, Zhou P, Cong L. Cryopreservation of immature and in-vitro matured human oocytes by vitrification. Reprod BioMed Online. 2009;19:369–373. doi: 10.1016/S1472-6483(10)60170-8.
    1. Fasano G, Demeestere I, Englert Y. In-vitro maturation of human oocytes: before or after vitrification? J Assist Reprod Genet. 2012;29:507–512. doi: 10.1007/s10815-012-9751-9.
    1. Wang H, Racowsky C, Combelles CMH. Is it best to cryopreserve human cumulus-free immature oocytes before or after in vitro maturation? Cryobiology. 2012;65:79–87. doi: 10.1016/j.cryobiol.2012.06.001.
    1. Benkhalifa M, Demirol A, Menezo Y, Balashova E, Abduljalil AK, Abbas S, et al. Natural cycle IVF and oocyte in-vitro maturation in polycystic ovary syndrome: a collaborative prospective study. ReprodBiomedOnline. 2009;18:29–36.
    1. Druckenmiller S, Goldman KN, Labella PA, Fino ME, Bazzocchi A, Noyes N. Successful oocyte cryopreservation in reproductive-aged cancer survivors. Obstet Gynecol. 2016;127:474–480. doi: 10.1097/AOG.0000000000001248.
    1. Cooper A, Paynter SJ, Fuller BJ, Shaw RW. Differential effects of cryopreservation on nuclear or cytoplasmic maturation in vitro in immature mouse oocytes from stimulated ovaries. Hum Reprod. 1998;13:971–978. doi: 10.1093/humrep/13.4.971.
    1. Hu W, Marchesi D, Qiao J, Feng HL. Effect of slow freeze versus vitrification on the oocyte: an animal model. Fertil Steril. 2012;98:752–760.e3. doi: 10.1016/j.fertnstert.2012.05.037.
    1. Matson PL, Graefling J, Junk SM, Yovich JL, Edirisinghe WR. Cryopreservation of oocytes and embryos: use of a mouse model to investigate effects upon zona hardness and formulate treatment strategies in an in-vitro fertilization programme. Hum Reprod. 1997;12:1550–1553. doi: 10.1093/humrep/12.7.1550.
    1. Gook DA, Osborn SM, Johnston WI. Cryopreservation of mouse and human oocytes using 1,2-propanediol and the configuration of the meiotic spindle. Hum Reprod. 1993;8:1101–1109. doi: 10.1093/oxfordjournals.humrep.a138201.
    1. Huang JYJ, Chen HY, Park JYS, Tan SL, Chian RC. Comparison of spindle and chromosome configuration in in vitro- and in vivo-matured mouse oocytes after vitrification. Fertil Steril. 2008;90:1424–1432. doi: 10.1016/j.fertnstert.2007.07.1335.
    1. Prasath EB, Chan MLH, Wong WHW, Lim CJW, Tharmalingam MD, Hendricks M, Loh SF, Chia YN. First pregnancy and live birth resulting from cryopreserved embryos obtained from in vitro matured oocytes after oophorectomy in an ovarian cancer patient. Hum Reprod. 2014;29:276–278. doi: 10.1093/humrep/det420.
    1. Uzelac PS, Delaney AA, Christensen GL, Bohler HCL, Nakajima ST. Live birth following in vitro maturation of oocytes retrieved from extracorporeal ovarian tissue aspiration and embryo cryopreservation for 5 years. Fertil Steril. 2015;104:1258–1260. doi: 10.1016/j.fertnstert.2015.07.1148.
    1. Dahan MH, Tan SL, Chung J, Son WY. Clinical definition paper on in vitro maturation of human oocytes. Hum Reprod. 2016;31:1383–1386. doi: 10.1093/humrep/dew109.

Source: PubMed

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